topfluor Search Results


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dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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Croda International Plc vesicles
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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Croda International Plc dipyrrometheneboron difluoride 24 norcholesterol bodipy labeled cholesterol avanti polar lipids inc alabaster al u s a
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
Dipyrrometheneboron Difluoride 24 Norcholesterol Bodipy Labeled Cholesterol Avanti Polar Lipids Inc Alabaster Al U S A, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Croda International Plc pi 4 5 p2
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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93
Croda International Plc ammonium salt pn
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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90
Croda International Plc topfluor ceramide
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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Croda International Plc fluorescent topfluor tmr
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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92
Croda International Plc oleoyl
dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor <t>C11</t> fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.
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Image Search Results


dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor C11 fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.

Journal: The Journal of Biological Chemistry

Article Title: Zebrafish are resilient to the loss of major diacylglycerol acyltransferase enzymes

doi: 10.1016/j.jbc.2024.107973

Figure Lengend Snippet: dga t2 mutant embryos are still capable of triacylglycerol synthesis in the YSL. A , TopFluor C11 fatty acid was mixed with canola oil and microinjected into the yolk mass of dgat2 sa13945 mutants and siblings at 3 dpf. B , representative image of the TopFluor C11 signal in a dgat2 sa13945 zebrafish yolk sac 4 h after injection; scale = 200 μm. C and D , lipids were extracted from TopFluor C11 injected embryos and separated by thin layer chromatography (20 pooled embryos per sample, wild-type and dgat2 sa13945/+ embryos were combined). C , lipid classes were identified using TopFluor-labeled lipid standards. Arrow indicates TAG synthesized from TopFlour C11 in vivo . (CE: cholesterol ester, TAG: triacylglycerol, DAG: diacylglycerol, MAG: monoacylglycerol; PL: phospholipid; ∗ denote autofluorescent lipids in the fish lysate). D , the quantity of lipids in each class was quantified and expressed as a percent of total fluorescent lipids; N = 6 experiments, mean ± SD, Two-way ANOVA, not significantly different, p = 0.9929. Note, it appears that the TopFluor C11 is unable to be incorporated into cholesterol esters. E , large lipid droplets were manually dissected out of dgat2 sa13945 larvae at 6 dpf (40–50 LDs from 30 – 40 larvae). Lipids in LDs or an equivalent volume of media surrounding dissected larvae were separated by multistep gradient HPLC and detected with a charged aerosol detector (CAD) in picoamperes (pA) as described in . The lipid droplet samples contain large quantities of triacylglycerols and cholesterol esters. Traces are representative of three independent experiments.

Article Snippet: TopFluor-labeled standards for TAG, CE, PC, DG, MG and C11 (TopFluor TAG: 810298; TopFluor CE: 810290; TopFluor PC: 810,281 TopFluor DG: 810300; MG: 810296; TopFluor C11 custom synthesis, Avanti Polar Lipids) were added to lipids extracted from uninjected embryos and loaded similarly onto the TLC plate.

Techniques: Mutagenesis, Injection, Thin Layer Chromatography, Labeling, Synthesized, In Vivo, Aerosol