Journal: Biomedicines

Article Title: The Imbalance of Astrocytic Mitochondrial Dynamics Following Blast-Induced Traumatic Brain Injury.

doi: 10.3390/biomedicines11020329

Figure Lengend Snippet: Figure 3. Mitochondrial Network Analysis (MiNA) software descriptors from astrocyte mitochondrial morphology post single mechanical exposure—in vitro model of bTBI. (a) Representative images (63x) of TOMM20 and skeletonized for data acquisition at 4 hours post single mechanical exposure. Astrocytes presented a significant increase in the number of individuals fragmented mitochondria (puncta and rod) and a small number of networks. (b) Representative images of TOMM20 and skeletonized for data acquisition at 24 h post single mechanical exposure. Astrocytes still presented a significant increase in the number of individuals fragmented mitochondria (puncta and rod), displayed

Article Snippet: Fixed cells were incubated, for three hours at 4°C, in blocking buffer with primary antibody TOMM20 at 1:100 dilution (Cat#: NBP181556; Novus Biologicals, Englewood, CO, USA) and GFAP at 1:100 dilution (Cat#: 13-0300; ThermoFisher, Waltham, MA, USA), this step was carefully conducted by placing the cov- Biomedicines 2023, 11, 329 7 of 21 erslip with upturned cells into a humidified chamber.

Techniques: Software, In Vitro

Journal: Molecular Vision

Article Title: A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model

doi:

Figure Lengend Snippet: Antibodies used for Flow-cytometry.

Article Snippet: CD45RA , IgG1 , Ox-33 , mouse , FITC , MCA430F , Serotec.

Techniques: Blocking Assay, Control

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A Chemically Defined, Xeno- and Blood-Free Culture Medium Sustains Increased Production of Small Extracellular Vesicles From Mesenchymal Stem Cells

doi: 10.3389/fbioe.2021.619930

Figure Lengend Snippet: Characterization of isolated UC-MSC-derived sEV produced in DMEM, Oxium TM EXO, and commercial medium. The sEV isolated from the different conditioned media were evaluated in terms of particle concentration, size, classical surface/interior markers, and morphology. (A) Histogram showing the particles’ concentrations according to their size. Violet line = DMEM-derived sEV; orange line = Oxium TM EXO-derived sEV; light blue line = commercial medium-derived sEV. The mean concentration obtained through NTA of five videos for each type of sEV is shown. (B) Size’s mean and mode obtained for each type of sEV. (C) Size’s mode (left panel) and standard deviation data (right panel) dispersion. (D) Percentage distribution of isolated particles’ concentrations according to their size: 0–50 nm, 51–200 nm, 201–300 nm, and >301 nm. (E) Representative histograms of median fluorescence intensity (MFI) obtained by flow cytometry of classical sEV surface markers. Gray = isotype control; violet = DMEM; orange = Oxium TM EXO, light blue = commercial medium. (F) Western blot, illustrating the presence of the sEV’s membrane-associated protein Flotillin-1 and the sEV’s luminal-scaffold protein Syntenin-1 (involved in sEV’s biogenesis). Note that in the isolated sEV there is minimal or no detectable contamination by Calnexin (endoplasmic reticulum) or TOMM20 (mitochondria), respectively. (G) Transmission electron microscopy (TEM) by uranyl acetate negative staining of isolated sEV from ultracentrifuge. The graphs show mean ± SEM. n = 2.

Article Snippet: Primary antibodies used were Syntenin-1 (1:1000; Novus Biologicals, Centennial, CO, United States, Cat. #NBP2-76873), Flotillin-1 (1:2000; Abcam Inc., Cambridge, MA, United States, Cat. #ab133497), Calnexin (1:2,000; Abcam Inc., Cambridge, MA, United States Cat. #ab22595), and TOMM20 (1:1,000; Novus Biologicals, Centennial, CO, United States, Cat. #NBP2-67501).

Techniques: Isolation, Derivative Assay, Produced, Concentration Assay, Standard Deviation, Dispersion, Fluorescence, Flow Cytometry, Control, Western Blot, Membrane, Transmission Assay, Electron Microscopy, Negative Staining