tom20 rabbit Search Results


tom20  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc tom20
A , B MDA-MB-231 and MCF-7 cells were treated with or without flubendazole (0.5 μM) for 24 h. A fluorescence microscope evaluated the intensity of Calcein AM. Representative images and quantification of Calcein AM were shown. Scale bar, 5 µm. C , D Flow cytometric analysis and quantification of mitochondrial membrane potential changes in MDA-MB-231 and MCF-7 cells treated with or without flubendazole (0.5 μM) for 24 h. E Mitochondria were stained with MitoTracker TM Deep Red FM probes for 30 min and observed with a confocal microscope. Representative images of mitochondrial morphology were shown. Scale bar, 5 µm. F RT-qPCR analysis of mitochondrial DNA copies in MDA-MB-231 and MCF-7 cells. G , H Mitochondria were stained with MitoSOX TM Red FM for 30 min, and mitochondrial ROS accumulation was analyzed by flow cytometry. I ATP content measurement in MDA-MB-231 and MCF-7 cells treated with or without flubendazole (0.5 μM) for 24 h. J Immunoblotting analysis of VDAC1, SOD2, COX IV, <t>TOM20</t> expression in MDA-MB-231 and MCF-7 cells treated with the indicated concentration of flubendazole for 24 h. β-actin was used as the loading control. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).
Tom20, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tom20/product/Cell Signaling Technology Inc
Average 99 stars, based on 1 article reviews
tom20 - by Bioz Stars, 2026-03
99/100 stars
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rabbit anti-human tom20  (abberior instruments)
90
abberior instruments rabbit anti-human tom20
(A) Superresolution STED microscopy in cells transfected with ZnT9-50Met and ZnT9-50Val immunoassayed with anti-HA (green) and with the mitochondrial marker <t>anti-TOM20.</t> Scale bar = 10 µm. (B-C) Bar graph representing mean inter-mitochondrial distance (B) and relative mitochondria area (C) in cells transfected with ZnT9-50Met or ZnT9-50Val (n=10). *** p<0.001 using Bonferroni test between conditions. (D) Bar graph measuring FRET between the endoplasmic reticulum and mitochondria transfected with an empty vector (control), ZnT9-50Met or ZnT9-50Val together with the FEMP probe (n=46-65).
Rabbit Anti Human Tom20, supplied by abberior instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-human tom20/product/abberior instruments
Average 90 stars, based on 1 article reviews
rabbit anti-human tom20 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


A , B MDA-MB-231 and MCF-7 cells were treated with or without flubendazole (0.5 μM) for 24 h. A fluorescence microscope evaluated the intensity of Calcein AM. Representative images and quantification of Calcein AM were shown. Scale bar, 5 µm. C , D Flow cytometric analysis and quantification of mitochondrial membrane potential changes in MDA-MB-231 and MCF-7 cells treated with or without flubendazole (0.5 μM) for 24 h. E Mitochondria were stained with MitoTracker TM Deep Red FM probes for 30 min and observed with a confocal microscope. Representative images of mitochondrial morphology were shown. Scale bar, 5 µm. F RT-qPCR analysis of mitochondrial DNA copies in MDA-MB-231 and MCF-7 cells. G , H Mitochondria were stained with MitoSOX TM Red FM for 30 min, and mitochondrial ROS accumulation was analyzed by flow cytometry. I ATP content measurement in MDA-MB-231 and MCF-7 cells treated with or without flubendazole (0.5 μM) for 24 h. J Immunoblotting analysis of VDAC1, SOD2, COX IV, TOM20 expression in MDA-MB-231 and MCF-7 cells treated with the indicated concentration of flubendazole for 24 h. β-actin was used as the loading control. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Journal: Cell Death & Disease

Article Title: Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancer

doi: 10.1038/s41419-022-04823-8

Figure Lengend Snippet: A , B MDA-MB-231 and MCF-7 cells were treated with or without flubendazole (0.5 μM) for 24 h. A fluorescence microscope evaluated the intensity of Calcein AM. Representative images and quantification of Calcein AM were shown. Scale bar, 5 µm. C , D Flow cytometric analysis and quantification of mitochondrial membrane potential changes in MDA-MB-231 and MCF-7 cells treated with or without flubendazole (0.5 μM) for 24 h. E Mitochondria were stained with MitoTracker TM Deep Red FM probes for 30 min and observed with a confocal microscope. Representative images of mitochondrial morphology were shown. Scale bar, 5 µm. F RT-qPCR analysis of mitochondrial DNA copies in MDA-MB-231 and MCF-7 cells. G , H Mitochondria were stained with MitoSOX TM Red FM for 30 min, and mitochondrial ROS accumulation was analyzed by flow cytometry. I ATP content measurement in MDA-MB-231 and MCF-7 cells treated with or without flubendazole (0.5 μM) for 24 h. J Immunoblotting analysis of VDAC1, SOD2, COX IV, TOM20 expression in MDA-MB-231 and MCF-7 cells treated with the indicated concentration of flubendazole for 24 h. β-actin was used as the loading control. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Article Snippet: Antibodies used in this study were as follow: DRP1 (1:1000, 184247, Abcam, USA), p-DRP1 Ser616 (1:1000, 3455, CST, USA), Mitofusin-2 (1:1000, 9482, CST, USA), VDAC1 (1:1000, 55259, Proteintech, USA), SOD2 (1:1000, 13194, CST, USA), COX IV (1:1000, 4844, CST, USA), TOM20 (1:1000, 42406, CST, USA), PINK1 (1:500, 23274-1-AP, Proteintech), Parkin (1:500, 14060-1-AP, Proteintech, USA), p-Parkin ser65 (1:1000, 36866, CST, USA), LC3 (1:1000, 51520, Abcam, USA), p62 (1:1000, 8025, CST, USA), MMP-2 (1:1000, 87809, CST, USA), E-cadherin (1:1000, 14472, CST, USA), EVA1A (1:1000, 216043, Abcam, USA), β-actin (1:1000, 66009-1-Ig, Proteintech, USA), MitoTracker TM Deep Red FM (M22426, Invitrogen, USA).

Techniques: Fluorescence, Microscopy, Membrane, Staining, Quantitative RT-PCR, Flow Cytometry, Western Blot, Expressing, Concentration Assay, Control

A MDA-MB-231 and MCF-7 cells were treated with or without flubendazole (0.5 μM) for 24 h. The images were captured with a transmission electron microscope. Scale bar, 500 nm. B , C The autophagosomes are labeled by LC3 (green fluorescence) protein and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 5 µm. D Immunoblotting of PINK1, Parkin, p-Parkin ser65 and LC3 in MDA-MB-231 and MCF-7 cells treated with the indicated concentrations of flubendazole for 24 h. β-actin was used as the loading control. E Immunoblotting of Parkin in the cytosolic and mitochondrial fractions of MDA-MB-231 and MCF-7 cells treated with or without Flubendazole (0.5 μM) for 24 h. β-actin (cytoplasmic fraction) and VDAC1 (mitochondrial fraction) were used as the loading controls. F , G Colocalization of PINK1 (green fluorescence) protein and Parkin (red fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized PINK1 and Parkin was quantified. Scale bar, 10 µm. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Journal: Cell Death & Disease

Article Title: Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancer

doi: 10.1038/s41419-022-04823-8

Figure Lengend Snippet: A MDA-MB-231 and MCF-7 cells were treated with or without flubendazole (0.5 μM) for 24 h. The images were captured with a transmission electron microscope. Scale bar, 500 nm. B , C The autophagosomes are labeled by LC3 (green fluorescence) protein and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 5 µm. D Immunoblotting of PINK1, Parkin, p-Parkin ser65 and LC3 in MDA-MB-231 and MCF-7 cells treated with the indicated concentrations of flubendazole for 24 h. β-actin was used as the loading control. E Immunoblotting of Parkin in the cytosolic and mitochondrial fractions of MDA-MB-231 and MCF-7 cells treated with or without Flubendazole (0.5 μM) for 24 h. β-actin (cytoplasmic fraction) and VDAC1 (mitochondrial fraction) were used as the loading controls. F , G Colocalization of PINK1 (green fluorescence) protein and Parkin (red fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized PINK1 and Parkin was quantified. Scale bar, 10 µm. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Article Snippet: Antibodies used in this study were as follow: DRP1 (1:1000, 184247, Abcam, USA), p-DRP1 Ser616 (1:1000, 3455, CST, USA), Mitofusin-2 (1:1000, 9482, CST, USA), VDAC1 (1:1000, 55259, Proteintech, USA), SOD2 (1:1000, 13194, CST, USA), COX IV (1:1000, 4844, CST, USA), TOM20 (1:1000, 42406, CST, USA), PINK1 (1:500, 23274-1-AP, Proteintech), Parkin (1:500, 14060-1-AP, Proteintech, USA), p-Parkin ser65 (1:1000, 36866, CST, USA), LC3 (1:1000, 51520, Abcam, USA), p62 (1:1000, 8025, CST, USA), MMP-2 (1:1000, 87809, CST, USA), E-cadherin (1:1000, 14472, CST, USA), EVA1A (1:1000, 216043, Abcam, USA), β-actin (1:1000, 66009-1-Ig, Proteintech, USA), MitoTracker TM Deep Red FM (M22426, Invitrogen, USA).

Techniques: Transmission Assay, Microscopy, Labeling, Fluorescence, Western Blot, Control

A Immunoblotting of DRP1, p-DRP1 ser616 and Mitofusin-2 in MDA-MB-231 and MCF-7 cells treated with the indicated concentrations of flubendazole for 24 h. β-actin was used as the loading control. B DRP1 mRNA expression in MDA-MB-231 and MCF-7 cells was analyzed by RT-qPCR. C , D MDA-MB-231 and MCF-7 cells were transfected with negative-control or DRP1 shRNA for 24 h, respectively. After treatment with or without flubendazole (0.5 μM) for 24 h. The autophagosomes are labeled by LC3 (green fluorescence) protein, and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 5 µm. E , F Colocalization of PINK1 (green fluorescence) protein and Parkin (red fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized PINK1 and Parkin was quantified. Scale bar, 10 µm. G Immunoblotting of Parkin, p-Parkin ser65 and PINK1 expression. β-actin was measured as the loading control. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Journal: Cell Death & Disease

Article Title: Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancer

doi: 10.1038/s41419-022-04823-8

Figure Lengend Snippet: A Immunoblotting of DRP1, p-DRP1 ser616 and Mitofusin-2 in MDA-MB-231 and MCF-7 cells treated with the indicated concentrations of flubendazole for 24 h. β-actin was used as the loading control. B DRP1 mRNA expression in MDA-MB-231 and MCF-7 cells was analyzed by RT-qPCR. C , D MDA-MB-231 and MCF-7 cells were transfected with negative-control or DRP1 shRNA for 24 h, respectively. After treatment with or without flubendazole (0.5 μM) for 24 h. The autophagosomes are labeled by LC3 (green fluorescence) protein, and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 5 µm. E , F Colocalization of PINK1 (green fluorescence) protein and Parkin (red fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized PINK1 and Parkin was quantified. Scale bar, 10 µm. G Immunoblotting of Parkin, p-Parkin ser65 and PINK1 expression. β-actin was measured as the loading control. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Article Snippet: Antibodies used in this study were as follow: DRP1 (1:1000, 184247, Abcam, USA), p-DRP1 Ser616 (1:1000, 3455, CST, USA), Mitofusin-2 (1:1000, 9482, CST, USA), VDAC1 (1:1000, 55259, Proteintech, USA), SOD2 (1:1000, 13194, CST, USA), COX IV (1:1000, 4844, CST, USA), TOM20 (1:1000, 42406, CST, USA), PINK1 (1:500, 23274-1-AP, Proteintech), Parkin (1:500, 14060-1-AP, Proteintech, USA), p-Parkin ser65 (1:1000, 36866, CST, USA), LC3 (1:1000, 51520, Abcam, USA), p62 (1:1000, 8025, CST, USA), MMP-2 (1:1000, 87809, CST, USA), E-cadherin (1:1000, 14472, CST, USA), EVA1A (1:1000, 216043, Abcam, USA), β-actin (1:1000, 66009-1-Ig, Proteintech, USA), MitoTracker TM Deep Red FM (M22426, Invitrogen, USA).

Techniques: Western Blot, Control, Expressing, Quantitative RT-PCR, Transfection, Negative Control, shRNA, Labeling, Fluorescence

MDA-MB-231 and MCF-7 cells were transfected with EVA1A siRNA or negative-control for 24 h, respectively, followed by treatment with or without flubendazole (0.5 μM). A Immunoblotting of EVA1A, DRP1, p-DRP1 ser616 , Parkin, p-Parkin ser65 and PINK1 expression. β-actin was measured as the loading control. B , C The autophagosomes are labeled by LC3 (green fluorescence) protein, and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 10 µm. D , E Colocalization of Parkin (red fluorescence) protein and TOM20 (green fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized Parkin and TOM20 was quantified. Scale bar, 10 µm. F ATP content measurement. G , H Flow cytometric analysis and quantification of mitochondrial membrane potential changes. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Journal: Cell Death & Disease

Article Title: Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancer

doi: 10.1038/s41419-022-04823-8

Figure Lengend Snippet: MDA-MB-231 and MCF-7 cells were transfected with EVA1A siRNA or negative-control for 24 h, respectively, followed by treatment with or without flubendazole (0.5 μM). A Immunoblotting of EVA1A, DRP1, p-DRP1 ser616 , Parkin, p-Parkin ser65 and PINK1 expression. β-actin was measured as the loading control. B , C The autophagosomes are labeled by LC3 (green fluorescence) protein, and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 10 µm. D , E Colocalization of Parkin (red fluorescence) protein and TOM20 (green fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized Parkin and TOM20 was quantified. Scale bar, 10 µm. F ATP content measurement. G , H Flow cytometric analysis and quantification of mitochondrial membrane potential changes. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Article Snippet: Antibodies used in this study were as follow: DRP1 (1:1000, 184247, Abcam, USA), p-DRP1 Ser616 (1:1000, 3455, CST, USA), Mitofusin-2 (1:1000, 9482, CST, USA), VDAC1 (1:1000, 55259, Proteintech, USA), SOD2 (1:1000, 13194, CST, USA), COX IV (1:1000, 4844, CST, USA), TOM20 (1:1000, 42406, CST, USA), PINK1 (1:500, 23274-1-AP, Proteintech), Parkin (1:500, 14060-1-AP, Proteintech, USA), p-Parkin ser65 (1:1000, 36866, CST, USA), LC3 (1:1000, 51520, Abcam, USA), p62 (1:1000, 8025, CST, USA), MMP-2 (1:1000, 87809, CST, USA), E-cadherin (1:1000, 14472, CST, USA), EVA1A (1:1000, 216043, Abcam, USA), β-actin (1:1000, 66009-1-Ig, Proteintech, USA), MitoTracker TM Deep Red FM (M22426, Invitrogen, USA).

Techniques: Transfection, Negative Control, Western Blot, Expressing, Control, Labeling, Fluorescence, Membrane

MDA-MB-231 and MCF-7 cells were co-transfected with DRP1 shRNA and Flag-EVA1A or vehicle control respectively for 48 h. A Immunoblotting of DRP1, EVA1A, p62, Parkin, p-Parkin ser65 , PINK1, and LC3 expression. β-actin was measured as the loading control. B , C The autophagosomes are labeled by LC3 (green fluorescence) protein, and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 10 µm. D , E Colocalization of Parkin (red fluorescence) protein and TOM20 (green fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized Parkin and TOM20 was quantified. Scale bar, 10 µm. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Journal: Cell Death & Disease

Article Title: Flubendazole induces mitochondrial dysfunction and DRP1-mediated mitophagy by targeting EVA1A in breast cancer

doi: 10.1038/s41419-022-04823-8

Figure Lengend Snippet: MDA-MB-231 and MCF-7 cells were co-transfected with DRP1 shRNA and Flag-EVA1A or vehicle control respectively for 48 h. A Immunoblotting of DRP1, EVA1A, p62, Parkin, p-Parkin ser65 , PINK1, and LC3 expression. β-actin was measured as the loading control. B , C The autophagosomes are labeled by LC3 (green fluorescence) protein, and the mitochondria are labeled by TOM20 (red fluorescence) protein. The number of co-localized LC3 and TOM20 was quantified. Scale bar, 10 µm. D , E Colocalization of Parkin (red fluorescence) protein and TOM20 (green fluorescence) protein in MDA-MB-231 and MCF-7 cells following flubendazole (0.5 μM, 24 h) treatment. The number of co-localized Parkin and TOM20 was quantified. Scale bar, 10 µm. Data represent mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance compared with respective control groups (all P -values were obtained by one-way ANOVA).

Article Snippet: Antibodies used in this study were as follow: DRP1 (1:1000, 184247, Abcam, USA), p-DRP1 Ser616 (1:1000, 3455, CST, USA), Mitofusin-2 (1:1000, 9482, CST, USA), VDAC1 (1:1000, 55259, Proteintech, USA), SOD2 (1:1000, 13194, CST, USA), COX IV (1:1000, 4844, CST, USA), TOM20 (1:1000, 42406, CST, USA), PINK1 (1:500, 23274-1-AP, Proteintech), Parkin (1:500, 14060-1-AP, Proteintech, USA), p-Parkin ser65 (1:1000, 36866, CST, USA), LC3 (1:1000, 51520, Abcam, USA), p62 (1:1000, 8025, CST, USA), MMP-2 (1:1000, 87809, CST, USA), E-cadherin (1:1000, 14472, CST, USA), EVA1A (1:1000, 216043, Abcam, USA), β-actin (1:1000, 66009-1-Ig, Proteintech, USA), MitoTracker TM Deep Red FM (M22426, Invitrogen, USA).

Techniques: Transfection, shRNA, Control, Western Blot, Expressing, Labeling, Fluorescence

(A) Superresolution STED microscopy in cells transfected with ZnT9-50Met and ZnT9-50Val immunoassayed with anti-HA (green) and with the mitochondrial marker anti-TOM20. Scale bar = 10 µm. (B-C) Bar graph representing mean inter-mitochondrial distance (B) and relative mitochondria area (C) in cells transfected with ZnT9-50Met or ZnT9-50Val (n=10). *** p<0.001 using Bonferroni test between conditions. (D) Bar graph measuring FRET between the endoplasmic reticulum and mitochondria transfected with an empty vector (control), ZnT9-50Met or ZnT9-50Val together with the FEMP probe (n=46-65).

Journal: bioRxiv

Article Title: Functional characterization of the Met50Val substitution in SLC30A9 as a novel case of adaptive introgression in humans

doi: 10.1101/2022.06.29.498106

Figure Lengend Snippet: (A) Superresolution STED microscopy in cells transfected with ZnT9-50Met and ZnT9-50Val immunoassayed with anti-HA (green) and with the mitochondrial marker anti-TOM20. Scale bar = 10 µm. (B-C) Bar graph representing mean inter-mitochondrial distance (B) and relative mitochondria area (C) in cells transfected with ZnT9-50Met or ZnT9-50Val (n=10). *** p<0.001 using Bonferroni test between conditions. (D) Bar graph measuring FRET between the endoplasmic reticulum and mitochondria transfected with an empty vector (control), ZnT9-50Met or ZnT9-50Val together with the FEMP probe (n=46-65).

Article Snippet: We used the rabbit anti-human TOM20 and the rabbit anti-human Calreticulin antibodies (1:1000) and secondary antibodies Abberior STAR RED or ORANGE (1:350).

Techniques: Microscopy, Transfection, Marker, Plasmid Preparation