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    SCIEX tof tof 5800
    Conjugation of TCO and azide to an anti-EGFR antibody using standard amine-reaction procedures. (A) Total attachment levels determined by <t>MALDI-TOF</t> versus the number of reaction equivalents, showing that modification efficiencies are similar. (B) Reaction
    Tof Tof 5800, supplied by SCIEX, used in various techniques. Bioz Stars score: 99/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Conjugation of TCO and azide to an anti-EGFR antibody using standard amine-reaction procedures. (A) Total attachment levels determined by MALDI-TOF versus the number of reaction equivalents, showing that modification efficiencies are similar. (B) Reaction

    Journal: Bioconjugate chemistry

    Article Title: Enhancing Reactivity for Bioorthogonal Pretargeting by Unmasking Antibody Conjugated trans-Cyclooctenes

    doi: 10.1021/bc500605g

    Figure Lengend Snippet: Conjugation of TCO and azide to an anti-EGFR antibody using standard amine-reaction procedures. (A) Total attachment levels determined by MALDI-TOF versus the number of reaction equivalents, showing that modification efficiencies are similar. (B) Reaction

    Article Snippet: The sample was loaded onto a AB SCIEX TOF/TOF 5800 MALDI-TOF spectrometer to obtain an average molecular weight for each antibody.

    Techniques: Conjugation Assay, Modification

    Translation of dual orthogonal coupling to different antibodies. (A) Anti-TfR and EpCAM antibodies had low TCO reactivity (12-15%) after direct amine-conjugation, but the PEG 24 -TCO increased reactive TCO density 3 to 4-fold. *MALDI-TOF analysis of the

    Journal: Bioconjugate chemistry

    Article Title: Enhancing Reactivity for Bioorthogonal Pretargeting by Unmasking Antibody Conjugated trans-Cyclooctenes

    doi: 10.1021/bc500605g

    Figure Lengend Snippet: Translation of dual orthogonal coupling to different antibodies. (A) Anti-TfR and EpCAM antibodies had low TCO reactivity (12-15%) after direct amine-conjugation, but the PEG 24 -TCO increased reactive TCO density 3 to 4-fold. *MALDI-TOF analysis of the

    Article Snippet: The sample was loaded onto a AB SCIEX TOF/TOF 5800 MALDI-TOF spectrometer to obtain an average molecular weight for each antibody.

    Techniques: Conjugation Assay

    Representative image of two-dimensional DIGE showing location of differential protein spots identified by MALDI-TOF/TOF. Porcine hepatocyte proteins in the control and 0.05 μg/ml T-2 treatment group were labeled with DIGE Fluor dye and separated

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Integrated Transcriptional and Proteomic Analysis with In Vitro Biochemical Assay Reveal the Important Role of CYP3A46 in T-2 Toxin Hydroxylation in Porcine Primary Hepatocytes *

    doi: 10.1074/mcp.M111.008748

    Figure Lengend Snippet: Representative image of two-dimensional DIGE showing location of differential protein spots identified by MALDI-TOF/TOF. Porcine hepatocyte proteins in the control and 0.05 μg/ml T-2 treatment group were labeled with DIGE Fluor dye and separated

    Article Snippet: Protein identification was performed on an AB SCIEX MALDI TOF-TOF™ 5800 Analyzer (AB SCIEX, Foster City, CA) equipped with a neodymium: yttrium-aluminum-garnet laser (laser wavelength was 349 nm).

    Techniques: Labeling

    Application of MIB/MS to analyze the kinomes of drug-sensitive and -resistant leukemia cells. Kinases from MYL and MYL-R cells were affinity enriched with multiplexed inhibitor beads and quantified by LC-MALDI TOF/TOF mass spectrometry (MIB/MS). Results from three independent experiments were pooled as described in Materials and Methods. ( A ) Over 150 kinases were quantified from MYL-R relative to MYL cells across a broad spectrum of kinase families, as depicted in the phylogenetic tree of the human protein kinase superfamily. Yellow and blue dots signify kinases that were increased or decreased in abundance, respectively, in MYL-R relative to MYL cells while gray dots signify kinases that were unchanged. Kinome illustration reproduced courtesy of Cell Signaling Technology, Inc. ( www.cellsignal.com ). ( B ) The trend of kinase abundance changes for all kinases quantified. Dashed line , ±1.5-fold change. ( C ) The kinome profile of MYL-R relative to MYL was derived from the kinases that were significantly changed after statistical analysis. The kinase abundance ratios and p-values from three independent experiments were combined and adjusted for multiple hypothesis testing and ratios with a Benjamini-Hochberg q-value

    Journal: PLoS ONE

    Article Title: Application of Multiplexed Kinase Inhibitor Beads to Study Kinome Adaptations in Drug-Resistant Leukemia

    doi: 10.1371/journal.pone.0066755

    Figure Lengend Snippet: Application of MIB/MS to analyze the kinomes of drug-sensitive and -resistant leukemia cells. Kinases from MYL and MYL-R cells were affinity enriched with multiplexed inhibitor beads and quantified by LC-MALDI TOF/TOF mass spectrometry (MIB/MS). Results from three independent experiments were pooled as described in Materials and Methods. ( A ) Over 150 kinases were quantified from MYL-R relative to MYL cells across a broad spectrum of kinase families, as depicted in the phylogenetic tree of the human protein kinase superfamily. Yellow and blue dots signify kinases that were increased or decreased in abundance, respectively, in MYL-R relative to MYL cells while gray dots signify kinases that were unchanged. Kinome illustration reproduced courtesy of Cell Signaling Technology, Inc. ( www.cellsignal.com ). ( B ) The trend of kinase abundance changes for all kinases quantified. Dashed line , ±1.5-fold change. ( C ) The kinome profile of MYL-R relative to MYL was derived from the kinases that were significantly changed after statistical analysis. The kinase abundance ratios and p-values from three independent experiments were combined and adjusted for multiple hypothesis testing and ratios with a Benjamini-Hochberg q-value

    Article Snippet: MS Analysis MS and MS/MS data were acquired on a MALDI TOF/TOF 5800 or 4800 (AB SCIEX).

    Techniques: Mass Spectrometry, Derivative Assay

    Characterisation of spider venom polyamines. ( A ) RP-HPLC chromatogram of crude female Phlogius sp. venom with the peak corresponding to the polyamine PA 366 highlighted in red (Thermo Scientific Hypersil GOLD aQ 250 × 10 mm, 5 µm column; 1 mL/min flow rate; Solvent A H 2 O/0.05% TFA, Solvent B 90% ACN/H 2 O/0.045% TFA; 5–80% solvent B in 75 min, 80–90% solvent B in 5 min, 90% solvent B for 5 min, and 90–5% solvent B in 2 min; absorbance at 214 nm); ( B ) SCIEX TOF/TOF™ 5800 MALDI MS/MS spectrum of PA 366 using CHCA matrix, and the determined chemical structure with relevant fragment ions highlighted; ( C ) RP-HPLC chromatogram of crude female A. robustus venom with the peak corresponding to the polyamine PA 389 highlighted in red (Thermo Scientific Hypersil GOLD aQ 250 × 10 mm, 5 µm column; 1 mL/min flow rate; Solvent A H 2 O/0.05% TFA, Solvent B 90% ACN/H 2 O/0.045% TFA; 5–80% solvent B in 75 min, 80–90% solvent B in 5 min, 90% solvent B for 5 min, and 90–5% solvent B in 2 min; absorbance at 214 nm); ( D ) SCIEX TOF/TOF™ 5800 MALDI-MS/MS spectrum of PA 389 using CHCA matrix, and the determined chemical structure with relevant fragment ions highlighted.

    Journal: Toxins

    Article Title: The Aromatic Head Group of Spider Toxin Polyamines Influences Toxicity to Cancer Cells

    doi: 10.3390/toxins9110346

    Figure Lengend Snippet: Characterisation of spider venom polyamines. ( A ) RP-HPLC chromatogram of crude female Phlogius sp. venom with the peak corresponding to the polyamine PA 366 highlighted in red (Thermo Scientific Hypersil GOLD aQ 250 × 10 mm, 5 µm column; 1 mL/min flow rate; Solvent A H 2 O/0.05% TFA, Solvent B 90% ACN/H 2 O/0.045% TFA; 5–80% solvent B in 75 min, 80–90% solvent B in 5 min, 90% solvent B for 5 min, and 90–5% solvent B in 2 min; absorbance at 214 nm); ( B ) SCIEX TOF/TOF™ 5800 MALDI MS/MS spectrum of PA 366 using CHCA matrix, and the determined chemical structure with relevant fragment ions highlighted; ( C ) RP-HPLC chromatogram of crude female A. robustus venom with the peak corresponding to the polyamine PA 389 highlighted in red (Thermo Scientific Hypersil GOLD aQ 250 × 10 mm, 5 µm column; 1 mL/min flow rate; Solvent A H 2 O/0.05% TFA, Solvent B 90% ACN/H 2 O/0.045% TFA; 5–80% solvent B in 75 min, 80–90% solvent B in 5 min, 90% solvent B for 5 min, and 90–5% solvent B in 2 min; absorbance at 214 nm); ( D ) SCIEX TOF/TOF™ 5800 MALDI-MS/MS spectrum of PA 389 using CHCA matrix, and the determined chemical structure with relevant fragment ions highlighted.

    Article Snippet: Successful labelling of PA366 was confirmed by molecular weight analysis using a SCIEX TOF/TOF™ 5800 mass spectrometer (SCIEX, Framingham, MA, USA), followed by RP-HPLC purification of labelled PA366 to remove excess non-reacted and hydrolyzed biotin reagent from the solution.

    Techniques: High Performance Liquid Chromatography, Flow Cytometry, Mass Spectrometry

    MALDI-MS Glycans. N-glycans were released by PNGase F, and sodium adducts were detected by positive ion mode MALDI-TOF-MS.

    Journal: mAbs

    Article Title: Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles—Part 2: Mass spectrometric methods

    doi: 10.1080/19420862.2015.1045173

    Figure Lengend Snippet: MALDI-MS Glycans. N-glycans were released by PNGase F, and sodium adducts were detected by positive ion mode MALDI-TOF-MS.

    Article Snippet: The mass spectra were obtained on a TOF/TOF™ 5800 MALDI-TOF System (AB SCIEX, Framingham) operated in the positive ion reflector mode.

    Techniques: Mass Spectrometry

    MALDI-MS Stabilized Glycans. N-glycans were released by PNGase F, sialic acids were stabilized by lactonization, and sodium adducts were detected by positive ion mode MALDI-TOF-MS.

    Journal: mAbs

    Article Title: Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles—Part 2: Mass spectrometric methods

    doi: 10.1080/19420862.2015.1045173

    Figure Lengend Snippet: MALDI-MS Stabilized Glycans. N-glycans were released by PNGase F, sialic acids were stabilized by lactonization, and sodium adducts were detected by positive ion mode MALDI-TOF-MS.

    Article Snippet: The mass spectra were obtained on a TOF/TOF™ 5800 MALDI-TOF System (AB SCIEX, Framingham) operated in the positive ion reflector mode.

    Techniques: Mass Spectrometry