Journal: Aging (Albany NY)

Article Title: LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis

doi: 10.18632/aging.104162

Figure Lengend Snippet: Correlation analysis of MAGI2-AS3, miR-31-5p and TNS1 mRNA expression in TGCA-BCa patient tissues. ( A ) Volcanic map of differentially expressed mRNAs in BCa tissues compared to normal urothelial tissues. ( B ) Volcanic map of differentially expressed lncRNAs in BCa tissues compared to normal urothelial tissues. ( C ) Volcanic map of differentially expressed miRNAs in BCa tissues compared to normal urothelial tissues. Note: p<0.05 and logFC>2 were used as threshold parameters. Green dots represent down-regulated RNAs, and red dots represent up-regulated RNAs. ( D ) Distribution map shows correlation between MAGI2-AS3 and TNS1 expression in BCa patients from the TGCA database. Orange dots represent primary BCa samples and blue triangles represent normal urothelial tissue samples. ( E – G ) Histogram plots show the expression of MAGI2-AS3, mir-31-5p and TNS1 in BCa and normal bladder tissues in the TCGA database. The analyses were performed using the R software. * denotes p<0.05 and **denotes p<0.01.

Article Snippet: Then, the specimens were incubated with the anti-TNS1 antibody (Proteintech, Wuhan, China) overnight at 4 o C. Then, we incubated the specimens with the secondary antibody (Proteintech, Wuhan, China).

Techniques: Expressing, Software

Journal: Aging (Albany NY)

Article Title: LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis

doi: 10.18632/aging.104162

Figure Lengend Snippet: Expression correlation between mRNA and lncRNA.

Article Snippet: Then, the specimens were incubated with the anti-TNS1 antibody (Proteintech, Wuhan, China) overnight at 4 o C. Then, we incubated the specimens with the secondary antibody (Proteintech, Wuhan, China).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis

doi: 10.18632/aging.104162

Figure Lengend Snippet: Expression levels of MAGI2-AS3, miR-31-5p and TNS1 in BCa tissues and cell lines. ( A – C ) QRT-PCR analysis results show the expression levels of MAGI2-AS3, miR-31-5p and TNS1 in BCa and adjacent normal tissues (n=45). ( D – F ) QRT-PCR analysis results show the expression levels of MAGI2-AS3, mir-31-5p and TNS1 in the normal urothelial cell line (SV-HUC-1) and BCa cell lines (T24 and J82). ( G ) Western blot analysis shows the expression of TNS1 in the normal urothelial cell line (SV-HUC-1) and BCa cell lines (T24 and J82). GAPDH was used as an internal control. *p<0.05. **p<0.01.

Article Snippet: Then, the specimens were incubated with the anti-TNS1 antibody (Proteintech, Wuhan, China) overnight at 4 o C. Then, we incubated the specimens with the secondary antibody (Proteintech, Wuhan, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Aging (Albany NY)

Article Title: LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis

doi: 10.18632/aging.104162

Figure Lengend Snippet: Characterization of the regulatory relationship between MAGI2-AS3, mir-31-5p and TNS1 in BCa cells. ( A – C ) QRT-PCR analysis shows the expression levels of MAGI2-AS3, miR-31-5p and TNS1 mRNA in control (si-NC-transfected), MAGI2-AS3 knockdown (siMAGI2-AS3-transfected) and MAGI2-AS3-overexpressing (oeMAGI2-AS3) T24 and J82 cell lines. ( D , E ) QRT-PCR analysis shows miR-31-5p and TNS1 mRNA levels in control, MAGI2-AS3-silenced, and MAGI2-AS3-silenced plus miR-31-5p-silenced T24 and J82 cell lines. ( F ) Luciferase reporter assay results show the relative luciferase activity in T24 cells transfected with vectors containing wild-type MAGI2-AS3 and control or miR-31-5p compared to those transfected with vectors containing mutant MAGI2-AS3 and control or miR-31-5p. ( G ) Luciferase reporter assay results show the relative luciferase activity in T24 cells transfected with vectors containing wild-type TNS1 and control or miR-31-5p compared to those transfected with mutated TNS1 and control or miR-31-5p. ( H , I ) RNA pull-down assay results show the relative levels of MAGI2-AS3 and TNS1 pulled down by NC-miR-31-5p, Bio-miR-31-5p-WT or Bio-miR-31-5p-Mut. ( J ) Representative western blot shows TNS1 protein levels in control, MAGI2-AS3 knockdown (siMAGI2-AS3-transfected), MAGI2-AS3-overexpressing (oeMAGI2-AS3), and MAGI2-AS3-overexpressing plus miR-31-5p-overexpressing (oeMAGI2-AS3 plus oemiR-31-5p) T24 and J82 cells. *p<0.05. **p<0.01. GAPDH was used as internal control.

Article Snippet: Then, the specimens were incubated with the anti-TNS1 antibody (Proteintech, Wuhan, China) overnight at 4 o C. Then, we incubated the specimens with the secondary antibody (Proteintech, Wuhan, China).

Techniques: Quantitative RT-PCR, Expressing, Control, Transfection, Knockdown, Luciferase, Reporter Assay, Activity Assay, Mutagenesis, Pull Down Assay, Western Blot

Journal: Aging (Albany NY)

Article Title: LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis

doi: 10.18632/aging.104162

Figure Lengend Snippet: Top GO terms associated with TNS1 and immunohistochemical analysis of TNS1 expression in BCa and normal urothelial tissues. ( A ) STRING database analysis shows the protein interaction network between TNS1 and TNS1-related proteins. The nodes represent proteins and the lines represent protein-protein interactions. ( B ) Top 10 GO terms related to TNS1 and TNS1-related proteins based on functional enrichment analysis using DAVID. The blue bars represent the number of TNS1-related genes in each GO term. ( C ) Immunohistochemical analysis results show TNS1 protein expression in BCa and adjacent normal urothelial tissues from T1, T2, T3 and T4 stage BCa patients.

Article Snippet: Then, the specimens were incubated with the anti-TNS1 antibody (Proteintech, Wuhan, China) overnight at 4 o C. Then, we incubated the specimens with the secondary antibody (Proteintech, Wuhan, China).

Techniques: Immunohistochemical staining, Expressing, Protein-Protein interactions, Functional Assay

Journal: Aging (Albany NY)

Article Title: LncRNA MAGI2-AS3 inhibits bladder cancer progression by targeting the miR-31-5p/TNS1 axis

doi: 10.18632/aging.104162

Figure Lengend Snippet: Correlation between TNS1 expression and clinicopathological factors.

Article Snippet: Then, the specimens were incubated with the anti-TNS1 antibody (Proteintech, Wuhan, China) overnight at 4 o C. Then, we incubated the specimens with the secondary antibody (Proteintech, Wuhan, China).

Techniques: Expressing