tnnt2 Search Results


96
Miltenyi Biotec rea400 cat
Rea400 Cat, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp tnnt2 cp04182357 g1
Gene Exp Tnnt2 Cp04182357 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio mouse troponin t
Mouse Troponin T, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio mouse troponin t elisa kit
Mouse Troponin T Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp tnnt2 mm00441922 m1
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Thermo Fisher gene exp tnnt2 hs00165960 m1
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Thermo Fisher gene exp tnnt2 mm01290256 m1
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Thermo Fisher gene exp tnnt2 hs00943911 m1
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96
Proteintech 15513 1 ap
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96
Miltenyi Biotec anti cardiac troponin t ctnt antibody
Fig. 6 | hiPSC-CM characterization. a, Brightfield images of hiPSC-CMs after 21 d of differentiation using the LI-BPEL protocol with lactate purification (left panel) or mBEL protocol (right panel). Scale bars, 200 µm. b, Expression of cardiac Troponin T <t>(cTnT)</t> by hiPSC-CMs. Representative flow cytometry analysis of hiPSC-CMs generated using either the LI-BPEL with lactate purification protocol (left panel) or mBEL (right panel). Histograms show hiPSC-CMs stained with isotype control (LI-BPEL with lactate purification) or unstained (mBEL) (gray), and stained with cTnT (red).
Anti Cardiac Troponin T Ctnt Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 6 | hiPSC-CM characterization. a, Brightfield images of hiPSC-CMs after 21 d of differentiation using the LI-BPEL protocol with lactate purification (left panel) or mBEL protocol (right panel). Scale bars, 200 µm. b, Expression of cardiac Troponin T (cTnT) by hiPSC-CMs. Representative flow cytometry analysis of hiPSC-CMs generated using either the LI-BPEL with lactate purification protocol (left panel) or mBEL (right panel). Histograms show hiPSC-CMs stained with isotype control (LI-BPEL with lactate purification) or unstained (mBEL) (gray), and stained with cTnT (red).

Journal: Nature Protocols

Article Title: Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells

doi: 10.1038/s41596-021-00497-2

Figure Lengend Snippet: Fig. 6 | hiPSC-CM characterization. a, Brightfield images of hiPSC-CMs after 21 d of differentiation using the LI-BPEL protocol with lactate purification (left panel) or mBEL protocol (right panel). Scale bars, 200 µm. b, Expression of cardiac Troponin T (cTnT) by hiPSC-CMs. Representative flow cytometry analysis of hiPSC-CMs generated using either the LI-BPEL with lactate purification protocol (left panel) or mBEL (right panel). Histograms show hiPSC-CMs stained with isotype control (LI-BPEL with lactate purification) or unstained (mBEL) (gray), and stained with cTnT (red).

Article Snippet: ● Anti-Troponin I antibody (H-170) (TNNI) (1:500 dilution; Santa Cruz Biotechnology, cat. no. sc-15368; RRID: AB_793465) ● Anti-alpha-actinin (Sarcomeric) antibody (ACTN2) (1:800 dilution; Sigma-Aldrich, cat. no. A7811; RRID: AB_476766) ● Anti-CD31/PECAM-1 antibody (1:200 dilution; R&D Systems, cat. no. AF806; RRID: AB_355617) NATURE PROTOCOLS PROTOCOL NATURE PROTOCOLS | VOL 16 |APRIL 2021 | 2213–2256 |www.nature.com/nprot 2225 ● Anti-Collagen Type I, clone 5D8-G9 antibody (COL1A1) (1:200 dilution; Millipore, cat. no. MAB3391; RRID: AB_94839) ● Anti-Wilms’ tumor protein antibody (WT1) (1:200 dilution; Millipore, cat. no. CA1026; RRID: AB_437848) ● Anti-TBX18 antibody (TBX18) (1:200 dilution; Sigma-Aldrich, cat. no. HPA029014; RRID: AB_10601597) ● Anti-ZO-1 antibody (ZO-1) (1:200 dilution; Thermo Fisher Scientific, cat. no. 33-9100; RRID: AB_87181) ● Anti-CX43/GJA1 antibody (1:200 dilution; Abcam, cat. no. ab11370; RRID: AB_297976) ● Anti-Vimentin, clone V9 antibody (VIM) (1:100 dilution; Sigma-Aldrich, cat. no. V6630; RRID: AB_477627) ● Anti-GATA4 (1:100 dilution; Santa Cruz Biotechnology, cat. no. sc-25310; RRID: AB_627667) ● Anti-cardiac Troponin T (cTnT) antibody (clone REA400)-VioBlue(1:50 dilution; Miltenyi Biotec, cat. no. 130-120-402; RRID: AB_2783891) ● REA Control Antibody (I), human IgG1 (clone REA293)-VioBlue (1:50 dilution; Miltenyi Biotec, cat. no. 130-108-346, RRID: AB_2661680) ● CD31 (PECAM-1) antibody (clone WM59)-APC (1:100 dilution; eBioscience, cat. no. 17-0319-42, RRID: AB_10852842) ● IgG1 kappa Isotype Control (clone P3.6.2.8.1)-APC (1:100 dilution; eBioscience, cat. no. 17-4714-42, RRID: AB_1603315)

Techniques: Expressing, Cytometry, Generated, Staining, Control