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Image Search Results
Journal: Nature Protocols
Article Title: Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells
doi: 10.1038/s41596-021-00497-2
Figure Lengend Snippet: Fig. 6 | hiPSC-CM characterization. a, Brightfield images of hiPSC-CMs after 21 d of differentiation using the LI-BPEL protocol with lactate purification (left panel) or mBEL protocol (right panel). Scale bars, 200 µm. b, Expression of cardiac Troponin T (cTnT) by hiPSC-CMs. Representative flow cytometry analysis of hiPSC-CMs generated using either the LI-BPEL with lactate purification protocol (left panel) or mBEL (right panel). Histograms show hiPSC-CMs stained with isotype control (LI-BPEL with lactate purification) or unstained (mBEL) (gray), and stained with cTnT (red).
Article Snippet: ● Anti-Troponin I antibody (H-170) (TNNI) (1:500 dilution; Santa Cruz Biotechnology, cat. no. sc-15368; RRID: AB_793465) ● Anti-alpha-actinin (Sarcomeric) antibody (ACTN2) (1:800 dilution; Sigma-Aldrich, cat. no. A7811; RRID: AB_476766) ● Anti-CD31/PECAM-1 antibody (1:200 dilution; R&D Systems, cat. no. AF806; RRID: AB_355617) NATURE PROTOCOLS PROTOCOL NATURE PROTOCOLS | VOL 16 |APRIL 2021 | 2213–2256 |www.nature.com/nprot 2225 ● Anti-Collagen Type I, clone 5D8-G9 antibody (COL1A1) (1:200 dilution; Millipore, cat. no. MAB3391; RRID: AB_94839) ● Anti-Wilms’ tumor protein antibody (WT1) (1:200 dilution; Millipore, cat. no. CA1026; RRID: AB_437848) ● Anti-TBX18 antibody (TBX18) (1:200 dilution; Sigma-Aldrich, cat. no. HPA029014; RRID: AB_10601597) ● Anti-ZO-1 antibody (ZO-1) (1:200 dilution; Thermo Fisher Scientific, cat. no. 33-9100; RRID: AB_87181) ● Anti-CX43/GJA1 antibody (1:200 dilution; Abcam, cat. no. ab11370; RRID: AB_297976) ● Anti-Vimentin, clone V9 antibody (VIM) (1:100 dilution; Sigma-Aldrich, cat. no. V6630; RRID: AB_477627) ● Anti-GATA4 (1:100 dilution; Santa Cruz Biotechnology, cat. no. sc-25310; RRID: AB_627667) ●
Techniques: Expressing, Cytometry, Generated, Staining, Control