tnfβ Search Results


92
R&D Systems anti tnf β
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Santa Cruz Biotechnology anti tnf β antibody
Primers used for qRT-PCR of ovine helper T (Th)1 and Th2 cytokines.
Anti Tnf β Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
Tnf α Elisa Kits, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human lymphotoxin α tnf β antibodies
Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
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Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
Csb E07379r, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
Human Tnf Elisa Kit, supplied by RayBiotech inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio tnf α
Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
Tnf α, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
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R&D Systems biotinylated polyclonal goat anti-murine tnf
Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
Biotinylated Polyclonal Goat Anti Murine Tnf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems human lymphotoxin alpha tnf beta duoset elisa
Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) <t>ELISA</t> was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
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Image Search Results


Primers used for qRT-PCR of ovine helper T (Th)1 and Th2 cytokines.

Journal: Animals : an Open Access Journal from MDPI

Article Title: Modulation of Helper T Cytokines in Thymus during Early Pregnancy in Ewes

doi: 10.3390/ani9050245

Figure Lengend Snippet: Primers used for qRT-PCR of ovine helper T (Th)1 and Th2 cytokines.

Article Snippet: Blot analysis was performed with an anti-IFN-γ antibody (Abcam, San Francisco, CA, USA, ab27919), an anti-TNF-β antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA, SC-28345), an anti-IL-2 antibody (Abcam, ab193807), an anti-IL-4 antibody (Bio-Techne, MAB2469), an anti-IL-5 antibody (Santa Cruz Biotechnology, SC-8433), an anti-IL-6 antibody (Abcam, ab193853), and an anti-IL-10 antibody (Santa Cruz Biotechnology, SC-32815) at a dilution of 1:1000 to analyze expression of Th1 and Th2 cytokines respectively.

Techniques: Sequencing

Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) ELISA was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).

Journal: Open Medicine

Article Title: The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A

doi: 10.1515/med-2023-0779

Figure Lengend Snippet: Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) ELISA was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).

Article Snippet: Human IL-6 and TNF-α ELISA kits were purchased from ABclonal Technology Co., Ltd (Wuhan, China), and human CXCL1 and CXCL3 ELISA kits were provided by MULTISCIENCES (LIANKE) Biotech, Co., Ltd (Hangzhou, China).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Transwell Assay, Migration, Western Blot

Overexpression of GADD45A promoted inflammatory molecule secretion and EMT of lung cells. (a) Cells were transfected with GADD45A overexpression vector, and qPCR was performed to detect the transfection effect ( n = 3). After cells overexpressed with GADD45A, (b) ELISA was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).

Journal: Open Medicine

Article Title: The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A

doi: 10.1515/med-2023-0779

Figure Lengend Snippet: Overexpression of GADD45A promoted inflammatory molecule secretion and EMT of lung cells. (a) Cells were transfected with GADD45A overexpression vector, and qPCR was performed to detect the transfection effect ( n = 3). After cells overexpressed with GADD45A, (b) ELISA was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).

Article Snippet: Human IL-6 and TNF-α ELISA kits were purchased from ABclonal Technology Co., Ltd (Wuhan, China), and human CXCL1 and CXCL3 ELISA kits were provided by MULTISCIENCES (LIANKE) Biotech, Co., Ltd (Hangzhou, China).

Techniques: Over Expression, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Transwell Assay, Migration, Western Blot