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Image Search Results
Journal: Animals : an Open Access Journal from MDPI
Article Title: Modulation of Helper T Cytokines in Thymus during Early Pregnancy in Ewes
doi: 10.3390/ani9050245
Figure Lengend Snippet: Primers used for qRT-PCR of ovine helper T (Th)1 and Th2 cytokines.
Article Snippet: Blot analysis was performed with an anti-IFN-γ antibody (Abcam, San Francisco, CA, USA, ab27919), an
Techniques: Sequencing
Journal: Open Medicine
Article Title: The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A
doi: 10.1515/med-2023-0779
Figure Lengend Snippet: Spike protein induced inflammatory molecule secretion and EMT of lung cells. (a) Cells were co-cultured with different concentrations of S protein for 24, 48, and 72 h. MTT was used to detect cell viability ( n = 3). After cells were treated with 1,000 ng/mL S protein for 48 h, (b) ELISA was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
Article Snippet: Human IL-6 and
Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Transwell Assay, Migration, Western Blot
Journal: Open Medicine
Article Title: The spike protein of SARS-CoV-2 induces inflammation and EMT of lung epithelial cells and fibroblasts through the upregulation of GADD45A
doi: 10.1515/med-2023-0779
Figure Lengend Snippet: Overexpression of GADD45A promoted inflammatory molecule secretion and EMT of lung cells. (a) Cells were transfected with GADD45A overexpression vector, and qPCR was performed to detect the transfection effect ( n = 3). After cells overexpressed with GADD45A, (b) ELISA was used to analyze the supernatant levels of TNF-α, IL-6, CXCL1, and CXCL2 ( n = 3). (c) Transwell assay was performed to detect migration ability ( n = 3) and (d) western blotting was used to detect the protein levels of E-cadherin, vimentin, and N-cadherin ( n = 3).
Article Snippet: Human IL-6 and
Techniques: Over Expression, Transfection, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Transwell Assay, Migration, Western Blot