Journal: Cell Death & Disease

Article Title: PA-MSHA induces inflamed tumor microenvironment and sensitizes tumor to anti-PD-1 therapy

doi: 10.1038/s41419-022-05368-6

Figure Lengend Snippet: Primer sequences of TLR1-10, IL-1β, IL-6, IL-12α, IL-12β, CXCL9-11 and GAPDH.

Article Snippet: The NF-κB inhibitor BAY 11-7082, TLR4 inhibitor Resatorvid, TLR5 inhibitor TH1020 and TLR8 inhibitor CU-CPT-8m were purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques:

Journal: Journal of Clinical Investigation

Article Title: p53-responsive TLR8 SNP enhances human innate immune response to respiratory syncytial virus

doi: 10.1172/jci128626

Figure Lengend Snippet: Figure 2. Activation of p53 by chemothera- peutic drugs amplifies the TLR8-mediated immune response in human lymphocytes in a SNP rs3761624–dependent manner. (A) IL-6 secretion measured by ELISA in blinded samples from PHA-stimulated lymphocytes pretreated with DMSO, nutlin, DXR, or IR for 24 hours then washed twice with PBS and challenged with water, TLR8 PAMP ssRNA40 (5 μg/mL) or its decoy ssRNA41 (5 μg/mL) for 3 hours. Each dot represents a different donor (n = 27). Presented are IL-6 secretion profiles (n = 25) grouped by A/G rs3761624 genotypes after (B) nutlin, (C) DXR, and (D) IR treatments for 24 hours. #P < 0.001 for drug-treated samples when compared with NT or DMSO treatments after ssRNA40 challenge for each genotype (2-tailed unpaired Student’s t test). *P < 0.01; **P < 0.001; ***P < 0.0001 when compared with drug- and ssRNA40-treated group expressing the A (male) or AA (female) rs3761624 SNP allele (s) (Wilcoxon signed-rank test).

Article Snippet: Direct intracellular staining was performed using a conjugated TLR8 antibody (44C143) (Alexa Fluor 647; Novus) to evaluate changes in TLR8 protein expression.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Journal of Clinical Investigation

Article Title: p53-responsive TLR8 SNP enhances human innate immune response to respiratory syncytial virus

doi: 10.1172/jci128626

Figure Lengend Snippet: Figure 3. TLR8 rs3761624 polymorphism con- tributes to severity of RSV infection in infants. DNA was isolated from whole blood, and infants with mild or severe RSV infection were geno- typed for TLR8 rs3761624 SNP. χ2 analysis of RSV disease severity among (A) female and (B) male homozygous WT, heterozygous, and homozy- gous variants was used to determine association of genotype with increased disease severity. The percentage of patients for each genotype is shown below each bar.

Article Snippet: Direct intracellular staining was performed using a conjugated TLR8 antibody (44C143) (Alexa Fluor 647; Novus) to evaluate changes in TLR8 protein expression.

Techniques: Infection, Isolation