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  • 99
    Millipore thin layer chromatography tlc
    Thin Layer Chromatography Tlc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck & Co tlc
    Tlc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 1750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck KGaA tlc
    Tlc, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck & Co thin layer chromatography tlc plates
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
    Thin Layer Chromatography Tlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Camag tlc scanner 3
    Iterative <t>TLC</t> analyses of culture extracts for the identification of a flavonoid-modifying enzyme. Cells were grown in LB medium with appropriate antibiotics. Biotransformation was performed as described in Materials and Methods. Culture extracts in EtOAc from 24-h biotransformation reactions with 100 μM quercetin as a substrate were applied on Merck silica gel 60 F 254 TLC plates. UV chromatograms are displayed in relative absorbance units (AU) versus the R f value measured at 365 nm on a densitometric TLC <t>Scanner</t> 3 (Camag, Muttenz, Switzerland) for activity determination. Peaks of the remaining quercetin substrate are depicted in light gray near the solvent front (Q); product peaks are shown in dark gray (P1, P2, and P3). TLC analyses of culture extracts from the following biotransformations led to the final isolation of the GT-encoding ORF gtfC : pool MT144R of 48 fosmid clones within the positive clone pFOS144C11 (A), pool MT144C of six fosmid clones within the positive clone (B), the positive single fosmid clone pFOS144C11 (C), the positive subclone pSK144C11 derived from pFOS144C11 (D), and the active ORF gtfC derived from pFOS144C11 in clone pD gtfC (E).
    Tlc Scanner 3, supplied by Camag, used in various techniques. Bioz Stars score: 92/100, based on 906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck KGaA tlc plates
    Beta 2 -glycoprotein I (β 2 -GPI) dependence of immunoreactivity to cardiolipin (CL) derivatives. (a) Binding of β 2 -GPI to CL and its derivatives. Intriguingly, β 2 -GPI showed significant binding to CL, monolysocardiolipin (MCL), and dilysocardiolipin (DCL), which was comparable with that for CL at the highest concentration tested. Each data point represents the mean of triplicate determinations. (b) Box-and-whisker plot of antimonolysocardiolipin antibody (aMCL) binding in six antiphospholipid syndrome (APS) patients. Median, quartiles, range, and possibly extreme values are shown. The blocking and the washing steps were performed with foetal calf serum (FCS) (10%) in phosphate-buffered saline (PBS)-Tween-20 to provide the β 2 -GPI or with bovine serum albumin (BSA) (1%) or gelatine (0.5%) to avoid the presence of β 2 -GPI, which is commonly associated with FCS. No significant difference of aMCL reactivity was observed with different blocking solutions. (c) Thin-layer chromatography <t>(TLC)</t> <t>immunostaining</t> analysis of: lane 1: normal serum, diluted 1:100 in PBS/0.5% gelatine; lane 2: control positive aCL serum, diluted 1:100 in PBS/0.5% gelatine; lane 3: APS serum positive for both aCL and aMCL, diluted 1:100 in PBS/0.5% gelatine; lane 4: APS serum positive for both aCL and aMCL, diluted 1:1,000 in PBS/0.5% gelatine. No sera showed reactivity against DCL and hydrocardiolipin (HCL). The results in lanes 3 and 4 are representative of five different APS patients. OD, optical density; St, standard phospholipid visualisation of lipids (cardiolipin, hydrocardiolipin, monolysocardiolipin, and dilysocardiolipin) by iodide vapours.
    Tlc Plates, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 94/100, based on 735 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Merck & Co preparative tlc
    MALDI-TOF mass (a) and PSD fragment ion (b) spectra of band 3 of P. <t>furiosus</t> . (a): the lipid components present in band 3 (see <t>TLC</t> in Figure 3 ) were isolated and purified from the total lipid extract of the P. furiosus by preparative TLC. Peaks corresponding to the molecular ions of diphytanylglycerol analogue of phosphatidylinositol (PI) at m/z 893.4 and of the corresponding unsaturated species ( uns PI) at m/z 881.6. (b): peaks corresponding to the molecular ion of PIat m/z 893.45 plus the ion fragments corresponding to the diphytanylglycerol analogues of PA ( m/z 732.1) and to the sugar-phosphate residue ( m/z 241.3).
    Preparative Tlc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 94/100, based on 922 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore thin layer chromatography tlc plates
    A <t>TLC</t> plate with the extracts of the isolates grown in <t>YES</t> medium. The plate was photographed under UV light (312 nm) in a transilluminator. B 1 , B 2 , G 1 , and G 2 are the standards (1.5 μg) of aflatoxins (Sigma–Aldrich, Germany). The extract of A. parasiticus (UEM 443) reference isolate was also analyzed.
    Thin Layer Chromatography Tlc Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    MACHEREY NAGEL tlc
    A <t>TLC</t> plate with the extracts of the isolates grown in <t>YES</t> medium. The plate was photographed under UV light (312 nm) in a transilluminator. B 1 , B 2 , G 1 , and G 2 are the standards (1.5 μg) of aflatoxins (Sigma–Aldrich, Germany). The extract of A. parasiticus (UEM 443) reference isolate was also analyzed.
    Tlc, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Camag tlc scanner iii
    <t>Three</t> dimentional overlay of HPTLC densitograms of standard plot of tenoxicam. Analysis was done using 60F-254 <t>TLC</t> plates as stationery phase and toluene-ethyl acetate-formic acid (6:4:0.3, v/v/v) as mobile phase.
    Tlc Scanner Iii, supplied by Camag, used in various techniques. Bioz Stars score: 91/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Miles Scientific thin layer chromatography tlc
    <t>Three</t> dimentional overlay of HPTLC densitograms of standard plot of tenoxicam. Analysis was done using 60F-254 <t>TLC</t> plates as stationery phase and toluene-ethyl acetate-formic acid (6:4:0.3, v/v/v) as mobile phase.
    Thin Layer Chromatography Tlc, supplied by Miles Scientific, used in various techniques. Bioz Stars score: 93/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co precoated thin layer chromatography tlc plates
    <t>Three</t> dimentional overlay of HPTLC densitograms of standard plot of tenoxicam. Analysis was done using 60F-254 <t>TLC</t> plates as stationery phase and toluene-ethyl acetate-formic acid (6:4:0.3, v/v/v) as mobile phase.
    Precoated Thin Layer Chromatography Tlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Miles Scientific preparative tlc
    <t>TLC</t> analysis of purified <t>TMM,</t> TDM and CL derived from R. equi ATCC 33701. Unfractionated R. equi lipid (lane 1) and purified TMM, CL and TDM (lanes 2–4, respectively) were developed on TLC plates with the solvent system of chloroform/methanol/acetone/acetic acid (80 : 20 : 6 : 1, v/v), and detected with 20 % H 2 SO 4 (a) or Dittmer’s reagent (b).
    Preparative Tlc, supplied by Miles Scientific, used in various techniques. Bioz Stars score: 93/100, based on 322 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Camag automatic tlc sampler 4
    <t>TLC</t> analysis of purified <t>TMM,</t> TDM and CL derived from R. equi ATCC 33701. Unfractionated R. equi lipid (lane 1) and purified TMM, CL and TDM (lanes 2–4, respectively) were developed on TLC plates with the solvent system of chloroform/methanol/acetone/acetic acid (80 : 20 : 6 : 1, v/v), and detected with 20 % H 2 SO 4 (a) or Dittmer’s reagent (b).
    Automatic Tlc Sampler 4, supplied by Camag, used in various techniques. Bioz Stars score: 89/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Merck & Co silica gel 60 thin layer chromatography tlc plates
    Analysis of intracellular material of W303R/ MAL61 . Strains were grown in YEP-2% glucose medium; cells were harvested at an OD 600 of 0.4, washed twice, resuspended in an equal volume of YEP-2% maltose or YEP-2% glucose medium, and harvested after 4 h. Maltose and maltotriose permease activity assays were performed. The intracellular material was extracted and run on a silica <t>gel</t> 60 <t>TLC</t> plate; the solvent used was n -butanol-acetic acid-water (6:3:1). Control samples were run by using solutions of radiolabeled sugars. The chromatogram was visualized by using a PhosphorImager. A repeat of labeled glucose is contained in lane 12.
    Silica Gel 60 Thin Layer Chromatography Tlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck & Co silica gel 60 f254 tlc plates
    Analysis of intracellular material of W303R/ MAL61 . Strains were grown in YEP-2% glucose medium; cells were harvested at an OD 600 of 0.4, washed twice, resuspended in an equal volume of YEP-2% maltose or YEP-2% glucose medium, and harvested after 4 h. Maltose and maltotriose permease activity assays were performed. The intracellular material was extracted and run on a silica <t>gel</t> 60 <t>TLC</t> plate; the solvent used was n -butanol-acetic acid-water (6:3:1). Control samples were run by using solutions of radiolabeled sugars. The chromatogram was visualized by using a PhosphorImager. A repeat of labeled glucose is contained in lane 12.
    Silica Gel 60 F254 Tlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore analytical thin layer chromatography tlc
    Analysis of intracellular material of W303R/ MAL61 . Strains were grown in YEP-2% glucose medium; cells were harvested at an OD 600 of 0.4, washed twice, resuspended in an equal volume of YEP-2% maltose or YEP-2% glucose medium, and harvested after 4 h. Maltose and maltotriose permease activity assays were performed. The intracellular material was extracted and run on a silica <t>gel</t> 60 <t>TLC</t> plate; the solvent used was n -butanol-acetic acid-water (6:3:1). Control samples were run by using solutions of radiolabeled sugars. The chromatogram was visualized by using a PhosphorImager. A repeat of labeled glucose is contained in lane 12.
    Analytical Thin Layer Chromatography Tlc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Camag tlc plates
    Expression of ST, PN, and TR cluster genes in media containing H 2 O 2 . (A) Northern blots showing expression of the PN cluster gene ipnA , as well as actin and the PN cluster flanking gene AN2647.3, by wild-type A. <t>nidulans</t> grown for 24 h in medium containing, 0, 1, 2, or 3 mM H 2 O 2 . (B) Northern blots showing expression of the ST cluster gene aflR , the ST cluster flanking gene AN7830.3, the TR cluster gene tdiB , and actin by wild-type A. nidulans grown for 36 h in media with the same concentrations of H 2 O 2 as in panel A. RNA was extracted from liquid shake cultures in duplicate. (C) Histogram depicting quantified <t>TLC</t> data for ST production by wild-type A. nidulans after 72 h of growth on solid media containing the aforementioned concentrations of H 2 O 2 . Treatments were performed in triplicate. Production levels at 0 mM H 2 O 2 were assigned a value of 1, and all other production levels are presented relative to this. Different letters above the bars represent statistical differences at P
    Tlc Plates, supplied by Camag, used in various techniques. Bioz Stars score: 93/100, based on 226 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown.

    Journal: PLoS ONE

    Article Title: Establishment of HeLa Cell Mutants Deficient in Sphingolipid-Related Genes Using TALENs

    doi: 10.1371/journal.pone.0088124

    Figure Lengend Snippet: Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown.

    Article Snippet: Thin-layer chromatography (TLC) plates (Silica Gel 60) and High-performance TLC (HPTLC) plates (Silica Gel 60) were from Merck. l -[U-14 C]serine (5.957 GBq/mmol) was from Moravek (Brea, CA), and d -[1-14 C]galactose (2.072 GBq/mmol) was from GE Healthcare.

    Techniques: Isolation, Sequencing, Expressing, Staining, Labeling, Plasmid Preparation, Over Expression, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Isolation of UGCG -deficient and CERT/UGCG double-deficient clones. A, Target sites of TALEN-UGCG pair (TAL-ModA-UGCG) in human UGCG gene. The sequences are located in exon 6, which contains the codon of the 195th arginine (R195) essential for the activity. The target sites are shown in bold and the codon of R195 is shown in red. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on UGCG mRNA (accession number D50840). B, Surface expression of StxRs on TALEN-UGCG–treated HeLa cells. HeLa cells were treated with TALEN-UGCG (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on TAL-UGCG clones (TAL-UG#7 and #3) and a TAL-CERT/UGCG clone (TAL-CE#14UG#2). The clones were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of UGCG gene in TAL-UGCG (TAL-UG#7 and #3) and TAL-CE#14UG#2 clones. Deletions are shown in red and their lengths are specified on the right of the sequences. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-UG#7, -UG#3 and -CE#14UG#2 cells were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown. MGDG, monogalactosyl diacylglycerol; DGDG, digalactosyl diacylglycerol (Galα1-4GalDG); PC, phosphatidylcholine.

    Journal: PLoS ONE

    Article Title: Establishment of HeLa Cell Mutants Deficient in Sphingolipid-Related Genes Using TALENs

    doi: 10.1371/journal.pone.0088124

    Figure Lengend Snippet: Isolation of UGCG -deficient and CERT/UGCG double-deficient clones. A, Target sites of TALEN-UGCG pair (TAL-ModA-UGCG) in human UGCG gene. The sequences are located in exon 6, which contains the codon of the 195th arginine (R195) essential for the activity. The target sites are shown in bold and the codon of R195 is shown in red. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on UGCG mRNA (accession number D50840). B, Surface expression of StxRs on TALEN-UGCG–treated HeLa cells. HeLa cells were treated with TALEN-UGCG (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on TAL-UGCG clones (TAL-UG#7 and #3) and a TAL-CERT/UGCG clone (TAL-CE#14UG#2). The clones were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of UGCG gene in TAL-UGCG (TAL-UG#7 and #3) and TAL-CE#14UG#2 clones. Deletions are shown in red and their lengths are specified on the right of the sequences. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-UG#7, -UG#3 and -CE#14UG#2 cells were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown. MGDG, monogalactosyl diacylglycerol; DGDG, digalactosyl diacylglycerol (Galα1-4GalDG); PC, phosphatidylcholine.

    Article Snippet: Thin-layer chromatography (TLC) plates (Silica Gel 60) and High-performance TLC (HPTLC) plates (Silica Gel 60) were from Merck. l -[U-14 C]serine (5.957 GBq/mmol) was from Moravek (Brea, CA), and d -[1-14 C]galactose (2.072 GBq/mmol) was from GE Healthcare.

    Techniques: Isolation, Clone Assay, Activity Assay, Sequencing, Expressing, Staining, Labeling, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Formation of 14 C-labeled products from external [ 14 C]mannose. (A) MS4601. (B) MS4601/pMS104. (C) MS4601/pMS104/pMS101. In each experiment, 6 ml of cells (OD 600 , 2) was incubated with 0.14 μCi of [ 14 C]mannose, equivalent to 0.078 nmol. The supernatants of TCA-treated cells were analyzed by TLC and autoradiographed. Lanes contain samples from the following time points: 1, 10 s; 2, 1 min; 3, 5 min; 4, 15 min; and 5, 30 min. The positions of the known products are indicated. When GDP-mannose (GDP-man) was present (panels B and C), an additional product was formed and ran ahead of MG; it was tentatively assigned as fucose.

    Journal: Applied and Environmental Microbiology

    Article Title: Synthesis of GDP-Mannose and Mannosylglycerate from Labeled Mannose by Genetically Engineered Escherichia coli without Loss of Specific Isotopic Enrichment

    doi: 10.1128/AEM.69.1.233-240.2003

    Figure Lengend Snippet: Formation of 14 C-labeled products from external [ 14 C]mannose. (A) MS4601. (B) MS4601/pMS104. (C) MS4601/pMS104/pMS101. In each experiment, 6 ml of cells (OD 600 , 2) was incubated with 0.14 μCi of [ 14 C]mannose, equivalent to 0.078 nmol. The supernatants of TCA-treated cells were analyzed by TLC and autoradiographed. Lanes contain samples from the following time points: 1, 10 s; 2, 1 min; 3, 5 min; 4, 15 min; and 5, 30 min. The positions of the known products are indicated. When GDP-mannose (GDP-man) was present (panels B and C), an additional product was formed and ran ahead of MG; it was tentatively assigned as fucose.

    Article Snippet: After being dried, the TLC plate was autoradiographed for 3 days.

    Techniques: Labeling, Incubation, Thin Layer Chromatography

    Time-dependent formation of [ 14 C]MG from external [ 14 C]mannose in MS4601/pMS104/pMS101. In each experiment, 6 ml of cells (OD 600 , 1) was incubated with 0.14 μCi of [ 14 C]mannose. Unlabeled mannose was added at various concentrations: A, 0.078 μM (no addition of unlabeled mannose); B, 0.1 μM; C, 0.5 μM; D, 1 μM; E, 5 μM; and F, 10 μM. The supernatants of TCA-treated cells (5 μl) were analyzed by TLC and autoradiographed. Lanes contain samples from the following time points: 1, 10 s; 2, 5 min; 3, 20 min; 4, 40 min; and 5, 90 min.

    Journal: Applied and Environmental Microbiology

    Article Title: Synthesis of GDP-Mannose and Mannosylglycerate from Labeled Mannose by Genetically Engineered Escherichia coli without Loss of Specific Isotopic Enrichment

    doi: 10.1128/AEM.69.1.233-240.2003

    Figure Lengend Snippet: Time-dependent formation of [ 14 C]MG from external [ 14 C]mannose in MS4601/pMS104/pMS101. In each experiment, 6 ml of cells (OD 600 , 1) was incubated with 0.14 μCi of [ 14 C]mannose. Unlabeled mannose was added at various concentrations: A, 0.078 μM (no addition of unlabeled mannose); B, 0.1 μM; C, 0.5 μM; D, 1 μM; E, 5 μM; and F, 10 μM. The supernatants of TCA-treated cells (5 μl) were analyzed by TLC and autoradiographed. Lanes contain samples from the following time points: 1, 10 s; 2, 5 min; 3, 20 min; 4, 40 min; and 5, 90 min.

    Article Snippet: After being dried, the TLC plate was autoradiographed for 3 days.

    Techniques: Incubation, Thin Layer Chromatography

    Release of labeled compounds after treatment with ice-cold water. MS4601/pMS101/pMS104 was grown in NZA medium with 0.2% glucose. Washed cultures were incubated with 1, 5, and 20 μM [ 14 C]mannose containing the same total radioactivity (0.14 μCi). At the indicated times, 1-ml samples were withdrawn, harvested, and treated with ice-cold water. The supernatants were analyzed by TLC and autoradiographed. Time of harvest indicates the time of centrifugation; the radioactivity percentage indicates the amount of radioactivity with respect to the initial radioactivity present within the cells at the time indicated. Extraction with cold water released almost 100% of the radioactivity accumulated at the time. The radioactivity remaining in the medium was not analyzed. P, phosphate.

    Journal: Applied and Environmental Microbiology

    Article Title: Synthesis of GDP-Mannose and Mannosylglycerate from Labeled Mannose by Genetically Engineered Escherichia coli without Loss of Specific Isotopic Enrichment

    doi: 10.1128/AEM.69.1.233-240.2003

    Figure Lengend Snippet: Release of labeled compounds after treatment with ice-cold water. MS4601/pMS101/pMS104 was grown in NZA medium with 0.2% glucose. Washed cultures were incubated with 1, 5, and 20 μM [ 14 C]mannose containing the same total radioactivity (0.14 μCi). At the indicated times, 1-ml samples were withdrawn, harvested, and treated with ice-cold water. The supernatants were analyzed by TLC and autoradiographed. Time of harvest indicates the time of centrifugation; the radioactivity percentage indicates the amount of radioactivity with respect to the initial radioactivity present within the cells at the time indicated. Extraction with cold water released almost 100% of the radioactivity accumulated at the time. The radioactivity remaining in the medium was not analyzed. P, phosphate.

    Article Snippet: After being dried, the TLC plate was autoradiographed for 3 days.

    Techniques: Labeling, Incubation, Radioactivity, Thin Layer Chromatography, Centrifugation

    Iterative TLC analyses of culture extracts for the identification of a flavonoid-modifying enzyme. Cells were grown in LB medium with appropriate antibiotics. Biotransformation was performed as described in Materials and Methods. Culture extracts in EtOAc from 24-h biotransformation reactions with 100 μM quercetin as a substrate were applied on Merck silica gel 60 F 254 TLC plates. UV chromatograms are displayed in relative absorbance units (AU) versus the R f value measured at 365 nm on a densitometric TLC Scanner 3 (Camag, Muttenz, Switzerland) for activity determination. Peaks of the remaining quercetin substrate are depicted in light gray near the solvent front (Q); product peaks are shown in dark gray (P1, P2, and P3). TLC analyses of culture extracts from the following biotransformations led to the final isolation of the GT-encoding ORF gtfC : pool MT144R of 48 fosmid clones within the positive clone pFOS144C11 (A), pool MT144C of six fosmid clones within the positive clone (B), the positive single fosmid clone pFOS144C11 (C), the positive subclone pSK144C11 derived from pFOS144C11 (D), and the active ORF gtfC derived from pFOS144C11 in clone pD gtfC (E).

    Journal: Applied and Environmental Microbiology

    Article Title: Functional Screening of Metagenome and Genome Libraries for Detection of Novel Flavonoid-Modifying Enzymes

    doi: 10.1128/AEM.01077-13

    Figure Lengend Snippet: Iterative TLC analyses of culture extracts for the identification of a flavonoid-modifying enzyme. Cells were grown in LB medium with appropriate antibiotics. Biotransformation was performed as described in Materials and Methods. Culture extracts in EtOAc from 24-h biotransformation reactions with 100 μM quercetin as a substrate were applied on Merck silica gel 60 F 254 TLC plates. UV chromatograms are displayed in relative absorbance units (AU) versus the R f value measured at 365 nm on a densitometric TLC Scanner 3 (Camag, Muttenz, Switzerland) for activity determination. Peaks of the remaining quercetin substrate are depicted in light gray near the solvent front (Q); product peaks are shown in dark gray (P1, P2, and P3). TLC analyses of culture extracts from the following biotransformations led to the final isolation of the GT-encoding ORF gtfC : pool MT144R of 48 fosmid clones within the positive clone pFOS144C11 (A), pool MT144C of six fosmid clones within the positive clone (B), the positive single fosmid clone pFOS144C11 (C), the positive subclone pSK144C11 derived from pFOS144C11 (D), and the active ORF gtfC derived from pFOS144C11 in clone pD gtfC (E).

    Article Snippet: The absorbance of the separated bands was determined densitometrically depending on the absorbance maximum of the applied educts at 285 to 370 nm using a deuterium lamp in a TLC Scanner 3 (Camag, Muttenz, Switzerland).

    Techniques: Thin Layer Chromatography, Activity Assay, Isolation, Clone Assay, Derivative Assay

    Beta 2 -glycoprotein I (β 2 -GPI) dependence of immunoreactivity to cardiolipin (CL) derivatives. (a) Binding of β 2 -GPI to CL and its derivatives. Intriguingly, β 2 -GPI showed significant binding to CL, monolysocardiolipin (MCL), and dilysocardiolipin (DCL), which was comparable with that for CL at the highest concentration tested. Each data point represents the mean of triplicate determinations. (b) Box-and-whisker plot of antimonolysocardiolipin antibody (aMCL) binding in six antiphospholipid syndrome (APS) patients. Median, quartiles, range, and possibly extreme values are shown. The blocking and the washing steps were performed with foetal calf serum (FCS) (10%) in phosphate-buffered saline (PBS)-Tween-20 to provide the β 2 -GPI or with bovine serum albumin (BSA) (1%) or gelatine (0.5%) to avoid the presence of β 2 -GPI, which is commonly associated with FCS. No significant difference of aMCL reactivity was observed with different blocking solutions. (c) Thin-layer chromatography (TLC) immunostaining analysis of: lane 1: normal serum, diluted 1:100 in PBS/0.5% gelatine; lane 2: control positive aCL serum, diluted 1:100 in PBS/0.5% gelatine; lane 3: APS serum positive for both aCL and aMCL, diluted 1:100 in PBS/0.5% gelatine; lane 4: APS serum positive for both aCL and aMCL, diluted 1:1,000 in PBS/0.5% gelatine. No sera showed reactivity against DCL and hydrocardiolipin (HCL). The results in lanes 3 and 4 are representative of five different APS patients. OD, optical density; St, standard phospholipid visualisation of lipids (cardiolipin, hydrocardiolipin, monolysocardiolipin, and dilysocardiolipin) by iodide vapours.

    Journal: Arthritis Research & Therapy

    Article Title: Antiphospholipid reactivity against cardiolipin metabolites occurring during endothelial cell apoptosis

    doi: 10.1186/ar2091

    Figure Lengend Snippet: Beta 2 -glycoprotein I (β 2 -GPI) dependence of immunoreactivity to cardiolipin (CL) derivatives. (a) Binding of β 2 -GPI to CL and its derivatives. Intriguingly, β 2 -GPI showed significant binding to CL, monolysocardiolipin (MCL), and dilysocardiolipin (DCL), which was comparable with that for CL at the highest concentration tested. Each data point represents the mean of triplicate determinations. (b) Box-and-whisker plot of antimonolysocardiolipin antibody (aMCL) binding in six antiphospholipid syndrome (APS) patients. Median, quartiles, range, and possibly extreme values are shown. The blocking and the washing steps were performed with foetal calf serum (FCS) (10%) in phosphate-buffered saline (PBS)-Tween-20 to provide the β 2 -GPI or with bovine serum albumin (BSA) (1%) or gelatine (0.5%) to avoid the presence of β 2 -GPI, which is commonly associated with FCS. No significant difference of aMCL reactivity was observed with different blocking solutions. (c) Thin-layer chromatography (TLC) immunostaining analysis of: lane 1: normal serum, diluted 1:100 in PBS/0.5% gelatine; lane 2: control positive aCL serum, diluted 1:100 in PBS/0.5% gelatine; lane 3: APS serum positive for both aCL and aMCL, diluted 1:100 in PBS/0.5% gelatine; lane 4: APS serum positive for both aCL and aMCL, diluted 1:1,000 in PBS/0.5% gelatine. No sera showed reactivity against DCL and hydrocardiolipin (HCL). The results in lanes 3 and 4 are representative of five different APS patients. OD, optical density; St, standard phospholipid visualisation of lipids (cardiolipin, hydrocardiolipin, monolysocardiolipin, and dilysocardiolipin) by iodide vapours.

    Article Snippet: Immunostaining on TLC plates for aPLs The immunostaining of TLC plates (Merck, Darmstadt, Germany) was performed as described previously [ ], using 2.5 μg of CL, MCL, DCL, and HCL.

    Techniques: Binding Assay, Concentration Assay, Whisker Assay, Blocking Assay, Thin Layer Chromatography, Immunostaining

    MALDI-TOF mass (a) and PSD fragment ion (b) spectra of band 3 of P. furiosus . (a): the lipid components present in band 3 (see TLC in Figure 3 ) were isolated and purified from the total lipid extract of the P. furiosus by preparative TLC. Peaks corresponding to the molecular ions of diphytanylglycerol analogue of phosphatidylinositol (PI) at m/z 893.4 and of the corresponding unsaturated species ( uns PI) at m/z 881.6. (b): peaks corresponding to the molecular ion of PIat m/z 893.45 plus the ion fragments corresponding to the diphytanylglycerol analogues of PA ( m/z 732.1) and to the sugar-phosphate residue ( m/z 241.3).

    Journal: Archaea

    Article Title: Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

    doi: 10.1155/2012/957852

    Figure Lengend Snippet: MALDI-TOF mass (a) and PSD fragment ion (b) spectra of band 3 of P. furiosus . (a): the lipid components present in band 3 (see TLC in Figure 3 ) were isolated and purified from the total lipid extract of the P. furiosus by preparative TLC. Peaks corresponding to the molecular ions of diphytanylglycerol analogue of phosphatidylinositol (PI) at m/z 893.4 and of the corresponding unsaturated species ( uns PI) at m/z 881.6. (b): peaks corresponding to the molecular ion of PIat m/z 893.45 plus the ion fragments corresponding to the diphytanylglycerol analogues of PA ( m/z 732.1) and to the sugar-phosphate residue ( m/z 241.3).

    Article Snippet: Isolation and Purification of Individual Lipids from the Total Extract The lipid components of the total lipid extract of P. furiosus were separated by preparative TLC (Merck 20 × 20 cm × 0.2 mm thick layer, glass plates) in Solvent A. Lipids were visualized by staining with iodine vapour and were eluted and recovered from the scraped silica, as previously described [ ].

    Techniques: Thin Layer Chromatography, Isolation, Purification

    (a) Comparison of the MALDI-TOF mass spectra of the lipid component in band 7 of P. furiosus (see TLC in Figure 3 ) and the authentic standard BPG (i.e., diphytanylglycerol analogue of bisphosphatidylglycerol) isolated from Halobacterium salinarum . (b) PSD fragment ion spectrum of the diphytanylglycerol analogue of BPG of P. furiosus. Abbreviations: PA: diphytanylglycerol analogue of phosphatidic acid; PG: diphytanylglycerol analogue of phosphatidylglycerol; PGP: diphytanylglycerol analogue of phosphatidylglycerolphosphate.

    Journal: Archaea

    Article Title: Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

    doi: 10.1155/2012/957852

    Figure Lengend Snippet: (a) Comparison of the MALDI-TOF mass spectra of the lipid component in band 7 of P. furiosus (see TLC in Figure 3 ) and the authentic standard BPG (i.e., diphytanylglycerol analogue of bisphosphatidylglycerol) isolated from Halobacterium salinarum . (b) PSD fragment ion spectrum of the diphytanylglycerol analogue of BPG of P. furiosus. Abbreviations: PA: diphytanylglycerol analogue of phosphatidic acid; PG: diphytanylglycerol analogue of phosphatidylglycerol; PGP: diphytanylglycerol analogue of phosphatidylglycerolphosphate.

    Article Snippet: Isolation and Purification of Individual Lipids from the Total Extract The lipid components of the total lipid extract of P. furiosus were separated by preparative TLC (Merck 20 × 20 cm × 0.2 mm thick layer, glass plates) in Solvent A. Lipids were visualized by staining with iodine vapour and were eluted and recovered from the scraped silica, as previously described [ ].

    Techniques: Thin Layer Chromatography, Isolation

    Coupled MALDI-TOF/MS and HPTLC analyses of the total lipid extract of P. furiosus . The total lipid extract was applied on the TLC plate (200 μ g) in two lanes; the lipid bands in one lane were charred after spraying with 5% sulphuric acid, while 9-aminoacridine was applied manually along the other lane obtaining a continuous deposition. The main bands on the TLC plate after charring are shown in the centre of the picture, while, on the right and the left of the TLC, are shown the negative spectra obtained by MALDI scanning of the lane covered with the matrix. Dashed lines on the TLC plate were used to mark pale lipid bands.

    Journal: Archaea

    Article Title: Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus

    doi: 10.1155/2012/957852

    Figure Lengend Snippet: Coupled MALDI-TOF/MS and HPTLC analyses of the total lipid extract of P. furiosus . The total lipid extract was applied on the TLC plate (200 μ g) in two lanes; the lipid bands in one lane were charred after spraying with 5% sulphuric acid, while 9-aminoacridine was applied manually along the other lane obtaining a continuous deposition. The main bands on the TLC plate after charring are shown in the centre of the picture, while, on the right and the left of the TLC, are shown the negative spectra obtained by MALDI scanning of the lane covered with the matrix. Dashed lines on the TLC plate were used to mark pale lipid bands.

    Article Snippet: Isolation and Purification of Individual Lipids from the Total Extract The lipid components of the total lipid extract of P. furiosus were separated by preparative TLC (Merck 20 × 20 cm × 0.2 mm thick layer, glass plates) in Solvent A. Lipids were visualized by staining with iodine vapour and were eluted and recovered from the scraped silica, as previously described [ ].

    Techniques: Mass Spectrometry, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    A TLC plate with the extracts of the isolates grown in YES medium. The plate was photographed under UV light (312 nm) in a transilluminator. B 1 , B 2 , G 1 , and G 2 are the standards (1.5 μg) of aflatoxins (Sigma–Aldrich, Germany). The extract of A. parasiticus (UEM 443) reference isolate was also analyzed.

    Journal: Brazilian Journal of Microbiology

    Article Title: The occurrence of aflatoxigenic Aspergillus spp. in dairy cattle feed in Southern Brazil

    doi: 10.1016/j.bjm.2018.05.005

    Figure Lengend Snippet: A TLC plate with the extracts of the isolates grown in YES medium. The plate was photographed under UV light (312 nm) in a transilluminator. B 1 , B 2 , G 1 , and G 2 are the standards (1.5 μg) of aflatoxins (Sigma–Aldrich, Germany). The extract of A. parasiticus (UEM 443) reference isolate was also analyzed.

    Article Snippet: An aliquot of approximately 10 μL of the extracts obtained from the MGA, CMA, and YES cultures was added to Thin-Layer Chromatography (TLC) plates (Silica gel, Sigma–Aldrich, Germany).

    Techniques: Thin Layer Chromatography

    Three dimentional overlay of HPTLC densitograms of standard plot of tenoxicam. Analysis was done using 60F-254 TLC plates as stationery phase and toluene-ethyl acetate-formic acid (6:4:0.3, v/v/v) as mobile phase.

    Journal: Indian Journal of Pharmaceutical Sciences

    Article Title: Development and Validation of HPTLC Method for Estimation of Tenoxicam and its Formulations

    doi: 10.4103/0250-474X.102541

    Figure Lengend Snippet: Three dimentional overlay of HPTLC densitograms of standard plot of tenoxicam. Analysis was done using 60F-254 TLC plates as stationery phase and toluene-ethyl acetate-formic acid (6:4:0.3, v/v/v) as mobile phase.

    Article Snippet: After development, plates were air dried and densitometric scanning was performed at a wavelength of 379 nm with Camag TLC scanner III operated in the reflectance-absorbance mode and controlled by WinCATS software (V1.2.1).

    Techniques: High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Overlay of HPTLC densitograms of (a) Tenoxicam standard, (b) Formulation I, (c) Formulation II, (d) Formulation III. Analysis was done using 60F-254 TLC plates as stationery phase and toluene-ethyl acetate-formic acid (6:4:0.3, v/v/v) as mobile phase

    Journal: Indian Journal of Pharmaceutical Sciences

    Article Title: Development and Validation of HPTLC Method for Estimation of Tenoxicam and its Formulations

    doi: 10.4103/0250-474X.102541

    Figure Lengend Snippet: Overlay of HPTLC densitograms of (a) Tenoxicam standard, (b) Formulation I, (c) Formulation II, (d) Formulation III. Analysis was done using 60F-254 TLC plates as stationery phase and toluene-ethyl acetate-formic acid (6:4:0.3, v/v/v) as mobile phase

    Article Snippet: After development, plates were air dried and densitometric scanning was performed at a wavelength of 379 nm with Camag TLC scanner III operated in the reflectance-absorbance mode and controlled by WinCATS software (V1.2.1).

    Techniques: High Performance Thin Layer Chromatography, Thin Layer Chromatography

    TLC analysis of purified TMM, TDM and CL derived from R. equi ATCC 33701. Unfractionated R. equi lipid (lane 1) and purified TMM, CL and TDM (lanes 2–4, respectively) were developed on TLC plates with the solvent system of chloroform/methanol/acetone/acetic acid (80 : 20 : 6 : 1, v/v), and detected with 20 % H 2 SO 4 (a) or Dittmer’s reagent (b).

    Journal: Microbiology

    Article Title: Identification of Rhodococcus equi lipids recognized by host cytotoxic T lymphocytes

    doi: 10.1099/mic.0.035915-0

    Figure Lengend Snippet: TLC analysis of purified TMM, TDM and CL derived from R. equi ATCC 33701. Unfractionated R. equi lipid (lane 1) and purified TMM, CL and TDM (lanes 2–4, respectively) were developed on TLC plates with the solvent system of chloroform/methanol/acetone/acetic acid (80 : 20 : 6 : 1, v/v), and detected with 20 % H 2 SO 4 (a) or Dittmer’s reagent (b).

    Article Snippet: Briefly, TMM, TDM and CL were purified completely by preparative TLC on silica gel G (Uniplate; 20×20 cm, 250 µm; Analtech) until a single spot was obtained.

    Techniques: Thin Layer Chromatography, Purification, Derivative Assay

    Analysis of intracellular material of W303R/ MAL61 . Strains were grown in YEP-2% glucose medium; cells were harvested at an OD 600 of 0.4, washed twice, resuspended in an equal volume of YEP-2% maltose or YEP-2% glucose medium, and harvested after 4 h. Maltose and maltotriose permease activity assays were performed. The intracellular material was extracted and run on a silica gel 60 TLC plate; the solvent used was n -butanol-acetic acid-water (6:3:1). Control samples were run by using solutions of radiolabeled sugars. The chromatogram was visualized by using a PhosphorImager. A repeat of labeled glucose is contained in lane 12.

    Journal: Applied and Environmental Microbiology

    Article Title: Molecular Analysis of Maltotriose Transport and Utilization by Saccharomycescerevisiae

    doi: 10.1128/AEM.68.11.5326-5335.2002

    Figure Lengend Snippet: Analysis of intracellular material of W303R/ MAL61 . Strains were grown in YEP-2% glucose medium; cells were harvested at an OD 600 of 0.4, washed twice, resuspended in an equal volume of YEP-2% maltose or YEP-2% glucose medium, and harvested after 4 h. Maltose and maltotriose permease activity assays were performed. The intracellular material was extracted and run on a silica gel 60 TLC plate; the solvent used was n -butanol-acetic acid-water (6:3:1). Control samples were run by using solutions of radiolabeled sugars. The chromatogram was visualized by using a PhosphorImager. A repeat of labeled glucose is contained in lane 12.

    Article Snippet: Each extract was chromatographed on silica gel 60 thin-layer chromatography (TLC) plates (Merck) with a mobile phase of n -butanol- acetic acid-water (6:2:1 [vol/vol]) ( ).

    Techniques: Activity Assay, Thin Layer Chromatography, Labeling

    Expression of ST, PN, and TR cluster genes in media containing H 2 O 2 . (A) Northern blots showing expression of the PN cluster gene ipnA , as well as actin and the PN cluster flanking gene AN2647.3, by wild-type A. nidulans grown for 24 h in medium containing, 0, 1, 2, or 3 mM H 2 O 2 . (B) Northern blots showing expression of the ST cluster gene aflR , the ST cluster flanking gene AN7830.3, the TR cluster gene tdiB , and actin by wild-type A. nidulans grown for 36 h in media with the same concentrations of H 2 O 2 as in panel A. RNA was extracted from liquid shake cultures in duplicate. (C) Histogram depicting quantified TLC data for ST production by wild-type A. nidulans after 72 h of growth on solid media containing the aforementioned concentrations of H 2 O 2 . Treatments were performed in triplicate. Production levels at 0 mM H 2 O 2 were assigned a value of 1, and all other production levels are presented relative to this. Different letters above the bars represent statistical differences at P

    Journal: Eukaryotic Cell

    Article Title: Histone Deacetylase Activity Regulates Chemical Diversity in Aspergillus ▿

    doi: 10.1128/EC.00186-07

    Figure Lengend Snippet: Expression of ST, PN, and TR cluster genes in media containing H 2 O 2 . (A) Northern blots showing expression of the PN cluster gene ipnA , as well as actin and the PN cluster flanking gene AN2647.3, by wild-type A. nidulans grown for 24 h in medium containing, 0, 1, 2, or 3 mM H 2 O 2 . (B) Northern blots showing expression of the ST cluster gene aflR , the ST cluster flanking gene AN7830.3, the TR cluster gene tdiB , and actin by wild-type A. nidulans grown for 36 h in media with the same concentrations of H 2 O 2 as in panel A. RNA was extracted from liquid shake cultures in duplicate. (C) Histogram depicting quantified TLC data for ST production by wild-type A. nidulans after 72 h of growth on solid media containing the aforementioned concentrations of H 2 O 2 . Treatments were performed in triplicate. Production levels at 0 mM H 2 O 2 were assigned a value of 1, and all other production levels are presented relative to this. Different letters above the bars represent statistical differences at P

    Article Snippet: Quantification of ST and TR from A. nidulans extracts and unknown compounds from A. alternata and P. expansum on TLC plates was accomplished using a CAMAG II densitometer, according to the manufacturer's instructions.

    Techniques: Expressing, Northern Blot, Thin Layer Chromatography

    Effects of TSA on secondary metabolism of A. alternata and P. expansum . (A) Histogram of relative SM production levels in TSA-treated and untreated cultures. SM production levels in the absence of TSA were assigned a value of 1, and TSA-treated production levels are presented relative to untreated levels. The numbers on the x axis of the graph correspond to metabolites indicated on the TLC plates shown in panels B and C. The differences presented for individual compounds represent statistical differences at P

    Journal: Eukaryotic Cell

    Article Title: Histone Deacetylase Activity Regulates Chemical Diversity in Aspergillus ▿

    doi: 10.1128/EC.00186-07

    Figure Lengend Snippet: Effects of TSA on secondary metabolism of A. alternata and P. expansum . (A) Histogram of relative SM production levels in TSA-treated and untreated cultures. SM production levels in the absence of TSA were assigned a value of 1, and TSA-treated production levels are presented relative to untreated levels. The numbers on the x axis of the graph correspond to metabolites indicated on the TLC plates shown in panels B and C. The differences presented for individual compounds represent statistical differences at P

    Article Snippet: Quantification of ST and TR from A. nidulans extracts and unknown compounds from A. alternata and P. expansum on TLC plates was accomplished using a CAMAG II densitometer, according to the manufacturer's instructions.

    Techniques: Thin Layer Chromatography