Journal: Immunity, inflammation and disease

Article Title: Immune cell C/EBPβ deficiency is associated with hepatic mononuclear defects and spontaneous hepatitis but not steatohepatitis induced liver fibrosis.

doi: 10.1002/iid3.728

Figure Lengend Snippet: FIGURE 1 Altered hepatic myeloid cell populations in immune cell restricted C/EBPβ deficient mice. Single cell suspensions of hepatic cells were obtained 8 weeks after bone marrow transplantation of C/EBPβ−/−or C/EBPβ+/+ bone marrow into irradiated wild type recipients mice. Representative flow cytometry dot plots of singlets, CD45+ live (DAPI negative) cells including gating strategy for neutrophils, Ly6C+ monocytes, MHC II positive myeloid cells and Kupffer cells are depicted (A). Gating and representative dot plots of hepatic Ly6C−monocytes in indicated mice (B). Quantification of myeloid cell counts in the livers of indicated mice (C). Precent of TIM4+

Article Snippet: Anti‐mouse F4/80 (clone REA126) and anti‐mouse Tim4 (clone REA999) was purchased from Miltenyi Biotec.

Techniques: Transplantation Assay, Irradiation, Flow Cytometry

Journal: Disease Models & Mechanisms

Article Title: C-C motif receptor 2 is a core profibrotic factor in uremic cardiomyopathy

doi: 10.1242/dmm.052395

Figure Lengend Snippet: INCB3344 reduces LV inflammation and may increase LV residual macrophages. (A) Inhibition of CCR-2 downregulated the expression of markers of inflammation. (B) Inhibition of CCR-2 upregulated CCR-2 − residual macrophage markers in LV tissues. (C) Immunofluorescence showed increased infiltration of F4/80 + , TIMD-4 + and CD163 + cells in LV tissues after INCB3344 injection, and some F4/80 + cells were co-stained with CD163 and TIMD-4. White arrows indicate F4/80 + TIMD-4 + CD163 + cells (scale bar: 100 μm). (D) Flow cytometry showed that INCB3344 increased the proportion of CD163 + TIMD-4 + cells in F4/80 + cells derived from LV tissues. Sham, n =6; MNx, n =6; MNx+5w INCB3344, n =6; MNx+2w INCB3344, n =6. One-way ANOVA followed by Tukey's multiple comparisons test or Kruskal–Wallis test followed by Dunn's multiple comparisons test was used. * P <0.05, ** P <0.01.

Article Snippet: The slides were incubated overnight at 4°C with antibodies against TIMD-4 (1:200; Proteintech Group, Inc.; 12008-1-AP), followed by incubation with horseradish peroxidase (HRP)-linked goat anti-rabbit/mouse secondary antibodies at room temperature for 50 min after PBS washing.

Techniques: Inhibition, Expressing, Immunofluorescence, Injection, Staining, Flow Cytometry, Derivative Assay