Journal: Current Issues in Molecular Biology

Article Title: Effects of Aerobic Exercise on Irisin and Skeletal Muscle Autophagy in ApoE −/− Mice

doi: 10.3390/cimb47050371

Figure Lengend Snippet: Aerobic exercise did not have a particularly large effect on the AMPK/MTOR pathway in ApoE −/− mice. ( A – E ) Immunoblots for mTORC1, AMPK, p-AMPK, and LKB1 protein expression in the quadriceps femoris muscle of mice. Data presented as mean ± SD (n = 6). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Membranes were blocked with 5% non-fat milk in 1× TBST and incubated overnight at 4 °C with primary antibodies at the following dilutions: FNDC5(1:1000, 82671-1RR, Proteintech, Rosemont, IL, USA), LC3B (1:1000, #2775, Cell Signalling Technology, Danvers, MA, USA), p62 (1:5000, 18420-1-AP, Proteintech, Rosemont, IL, USA), AMPK (1:1000, #5832, Cell Signalling Technology, Danvers, MA, USA), p-AMPK (1:1000, #2535, Cell Signalling Technology, MA, USA), mTOR (7C10) (1:1000, #2983, Cell Signalling Technology, MA, USA), LKB1 (1:500, 10746-1-AP, Proteintech, Rosemont, IL, USA), and GAPDH (1:50000, 60004-1-Ig, Proteintech, Rosemont, IL, USA).

Techniques: Western Blot, Expressing