Journal: Journal of Cellular and Molecular Medicine

Article Title: Rosiglitazone via PPARγ-dependent suppression of oxidative stress attenuates endothelial dysfunction in rats fed homocysteine thiolactone

doi: 10.1111/jcmm.12510

Figure Lengend Snippet: HTL via PPARγ/NF-κB/NADPH oxidase induces oxidative stress in isolated rat aortas. The isolated rat aortic rings were exposed to HTL (1 mM) for 1 hr after preincubation with rosiglitazone (RSG, 0.1–1 mM), PDTC (0.1 mM) or apocynin (0.1 mM) for 30 min. Homogenates of aortic tissues were subjected to assay (A) nitric oxide productions by Griess method, (B) MDA content by TBA method and (C) SOD activity by the spectrophotometric method. All data were expressed as mean ± SEM. N is 5 in each group. * P < 0.05 versus Con, # P < 0.05 versus HTL alone.

Article Snippet: The contents of nitric oxide content (Cat: 10009419), MDA content (Cat: 700870), and SOD activity (Cat: 706002) in aortic tissues or serum were assayed by using commercial kits from Cayman Company (Ann Arbor, MI, USA).

Techniques: Isolation, Activity Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: Rosiglitazone via PPARγ-dependent suppression of oxidative stress attenuates endothelial dysfunction in rats fed homocysteine thiolactone

doi: 10.1111/jcmm.12510

Figure Lengend Snippet: Rosiglitazone suppresses oxidative stress and improves endothelial functions in rats fed HTL in vivo . The rats were intragastric gavaged HTL (50 mg/kg/d) and received administration of rosiglitazone (RSG, 20 mg/kg/d) for 8 weeks. At the end of experiments, rats were killed under anaesthesia. Artery from descending aorta was subjected to assay (A) endothelium-dependent relaxation induced by acetylcholine (Ach) and (B) endothelium-independent relaxation induced by sodium nitroprusside (SNP) in organ chamber. Blood was collected to assay (C) serum nitric oxide level by Griess method, (D) serum MDA content by TBA method and (E) SOD activity by the spectrophotometric method. All data were expressed as mean ± SEM. Five to ten rats in each group. * P < 0.05 versus Con, # P < 0.05 versus HTL alone. (F) Immunohistochemical analysis of active NF-κB in arteries by using phospho-p65 antibody. The picture (×40) is a representative picture from five independent experiments.

Article Snippet: The contents of nitric oxide content (Cat: 10009419), MDA content (Cat: 700870), and SOD activity (Cat: 706002) in aortic tissues or serum were assayed by using commercial kits from Cayman Company (Ann Arbor, MI, USA).

Techniques: In Vivo, Activity Assay, Immunohistochemical staining