thin-layer chromatography Search Results


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  • 99
    Millipore thin layer chromatography tlc
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    Merck & Co thin layer chromatography tlc
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    Merck KGaA thin layer chromatography tlc
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    Merck & Co thin layer chromatography
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    Merck KGaA thin layer chromatography
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    Millipore thin layer chromatography
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    MACHEREY NAGEL thin layer chromatography tlc
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    Miles Scientific thin layer chromatography tlc
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    MACHEREY NAGEL thin layer chromatography
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    Merck KGaA thin layer chromatography tlc plates
    <t>TLC</t> bioautography of the butanol extract of <t>DA12</t> against F. graminearum (A) and TLC analysis of the butanol extract of DA12 with iturin A (B). The TLC plates were developed in a solvent system of chloroform:methanol:water (14:6:1, v/v/v). The circle in panel A indicates a clear zone on the TLC plate.
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    SiliCycle thin layer chromatography tlc
    <t>TLC</t> bioautography of the butanol extract of <t>DA12</t> against F. graminearum (A) and TLC analysis of the butanol extract of DA12 with iturin A (B). The TLC plates were developed in a solvent system of chloroform:methanol:water (14:6:1, v/v/v). The circle in panel A indicates a clear zone on the TLC plate.
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    Merck & Co thin layer chromatography tlc plates
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
    Thin Layer Chromatography Tlc Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA thin layer chromatography plates
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
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    Merck & Co thin layer chromatography plates
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
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    Merck & Co preparative thin layer chromatography
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
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    Avantor thin layer chromatography tlc
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
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    Sorbent Technologies thin layer chromatography tlc
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
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    Miles Scientific preparative thin layer chromatography
    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by <t>HPTLC.</t> Radioactive image of an analyzed <t>TLC</t> plate is shown.
    Preparative Thin Layer Chromatography, supplied by Miles Scientific, used in various techniques. Bioz Stars score: 91/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    TLC bioautography of the butanol extract of DA12 against F. graminearum (A) and TLC analysis of the butanol extract of DA12 with iturin A (B). The TLC plates were developed in a solvent system of chloroform:methanol:water (14:6:1, v/v/v). The circle in panel A indicates a clear zone on the TLC plate.

    Journal: The Plant Pathology Journal

    Article Title: Characterization of Bacillus amyloliquefaciens DA12 Showing Potent Antifungal Activity against Mycotoxigenic Fusarium Species

    doi: 10.5423/PPJ.FT.06.2017.0126

    Figure Lengend Snippet: TLC bioautography of the butanol extract of DA12 against F. graminearum (A) and TLC analysis of the butanol extract of DA12 with iturin A (B). The TLC plates were developed in a solvent system of chloroform:methanol:water (14:6:1, v/v/v). The circle in panel A indicates a clear zone on the TLC plate.

    Article Snippet: Characterization of agar-diffusible antifungal metabolites In order to determine the agar diffusible antifungal metabolite using thin layer chromatography-direct bioautography, the butanol extract solution of DA12 (12 μl) was spotted onto a thin layer chromatography (TLC) plate (Kiegel 60, 0.25 mm thick, 1 cm × 8 cm; Merck, Germany) and developed in chloroform:methanol:water (14:6:1, v/v/v).

    Techniques: Thin Layer Chromatography

    Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown.

    Journal: PLoS ONE

    Article Title: Establishment of HeLa Cell Mutants Deficient in Sphingolipid-Related Genes Using TALENs

    doi: 10.1371/journal.pone.0088124

    Figure Lengend Snippet: Isolation of a B4GalT5 -deficient clone. A, Target sites of TALEN-B4G5 pair (TAL-ModA-B4GalT5) in human B4GalT5 gene. The sequences are located in exon 1, which codes part of the transmembrane domain. The target sites are shown in bold. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on B4GalT5 mRNA (accession number AB004550). B, Surface expression of StxRs on TALEN-B4GalT5–treated HeLa cells. HeLa cells were treated with TALEN-B4GalT5 (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on a TAL-B4GalT5 clone (TAL-B4G5#2). TAL-B4G5#2 cells were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of B4GalT5 gene in TAL-B4G5 clone (TAL-B4G5#2). The deletion is shown in red and its length specified on the right of the sequence. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-B4G5#2 clone and B4GalT5- or B4GalT6-restored TAL-B4G5#2 cells obtained by retroviral vector–mediated overexpression were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown.

    Article Snippet: Thin-layer chromatography (TLC) plates (Silica Gel 60) and High-performance TLC (HPTLC) plates (Silica Gel 60) were from Merck. l -[U-14 C]serine (5.957 GBq/mmol) was from Moravek (Brea, CA), and d -[1-14 C]galactose (2.072 GBq/mmol) was from GE Healthcare.

    Techniques: Isolation, Sequencing, Expressing, Staining, Labeling, Plasmid Preparation, Over Expression, High Performance Thin Layer Chromatography, Thin Layer Chromatography

    Isolation of UGCG -deficient and CERT/UGCG double-deficient clones. A, Target sites of TALEN-UGCG pair (TAL-ModA-UGCG) in human UGCG gene. The sequences are located in exon 6, which contains the codon of the 195th arginine (R195) essential for the activity. The target sites are shown in bold and the codon of R195 is shown in red. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on UGCG mRNA (accession number D50840). B, Surface expression of StxRs on TALEN-UGCG–treated HeLa cells. HeLa cells were treated with TALEN-UGCG (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on TAL-UGCG clones (TAL-UG#7 and #3) and a TAL-CERT/UGCG clone (TAL-CE#14UG#2). The clones were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of UGCG gene in TAL-UGCG (TAL-UG#7 and #3) and TAL-CE#14UG#2 clones. Deletions are shown in red and their lengths are specified on the right of the sequences. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-UG#7, -UG#3 and -CE#14UG#2 cells were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown. MGDG, monogalactosyl diacylglycerol; DGDG, digalactosyl diacylglycerol (Galα1-4GalDG); PC, phosphatidylcholine.

    Journal: PLoS ONE

    Article Title: Establishment of HeLa Cell Mutants Deficient in Sphingolipid-Related Genes Using TALENs

    doi: 10.1371/journal.pone.0088124

    Figure Lengend Snippet: Isolation of UGCG -deficient and CERT/UGCG double-deficient clones. A, Target sites of TALEN-UGCG pair (TAL-ModA-UGCG) in human UGCG gene. The sequences are located in exon 6, which contains the codon of the 195th arginine (R195) essential for the activity. The target sites are shown in bold and the codon of R195 is shown in red. The numbers on the right and left of the sequence indicate the sequence numbers from the A of the translation initiation codon, based on UGCG mRNA (accession number D50840). B, Surface expression of StxRs on TALEN-UGCG–treated HeLa cells. HeLa cells were treated with TALEN-UGCG (colored histogram with black line) or empty vectors (blue line), and the cells were stained with Alexa-555-Stx1B. C, Surface expression of StxRs on TAL-UGCG clones (TAL-UG#7 and #3) and a TAL-CERT/UGCG clone (TAL-CE#14UG#2). The clones were stained with Alexa-555-Stx1 B (colored histogram with black line) or not (magenta line) and HeLa-mCAT#8 cells were stained with Alexa-555-Stx1 B (blue line). D, Indel analysis of UGCG gene in TAL-UGCG (TAL-UG#7 and #3) and TAL-CE#14UG#2 clones. Deletions are shown in red and their lengths are specified on the right of the sequences. The predicted proteins are indicated based on the recommended description (see Materials and Methods ) [50] . E, Metabolic labeling of lipids with radioactive galactose. TAL-UG#7, -UG#3 and -CE#14UG#2 cells were labeled with [ 14 C]galactose for 16 h, and lipids extracted from the cells were separated by HPTLC. Radioactive image of an analyzed TLC plate is shown. MGDG, monogalactosyl diacylglycerol; DGDG, digalactosyl diacylglycerol (Galα1-4GalDG); PC, phosphatidylcholine.

    Article Snippet: Thin-layer chromatography (TLC) plates (Silica Gel 60) and High-performance TLC (HPTLC) plates (Silica Gel 60) were from Merck. l -[U-14 C]serine (5.957 GBq/mmol) was from Moravek (Brea, CA), and d -[1-14 C]galactose (2.072 GBq/mmol) was from GE Healthcare.

    Techniques: Isolation, Clone Assay, Activity Assay, Sequencing, Expressing, Staining, Labeling, High Performance Thin Layer Chromatography, Thin Layer Chromatography