Journal: Acta Crystallographica. Section F, Structural Biology Communications

Article Title: In situ crystal data-collection and ligand-screening system at SPring-8

doi: 10.1107/S2053230X22005283

Figure Lengend Snippet: Data-collection statistics of samples for equipment evaluation Values in parentheses are for the outer shell.

Article Snippet: To crystallize thermolysin, powdered thermolysin (catalog No. 201-08331, FUJIFILM Wako Pure Chemical Corporation, Japan) was dissolved to 25 mg ml −1 in 10 m M sodium hydroxide.

Techniques:

Journal: Biotechnology for Biofuels

Article Title: Chimeric cellobiohydrolase I expression, activity, and biochemical properties in three oleaginous yeast

doi: 10.1186/s13068-020-01856-z

Figure Lengend Snippet: a Thermolysin treatment. T0: initial sample before incubation. 0.5 h: Sample after 0.5-h incubation. b α-chymotrypsin treatment. T0: initial sample before incubation. 0.5 h: Sample after 0.5-h incubation

Article Snippet: Protein samples at a concentration of 0.25 mg/mL were incubated with Thermolysin (Promega, Madison, USA) or α-chymotrypsin (Promega, Madison, USA) at 25 μg/mL or 4 μg/mL, respectively, in a volume of 200 μL for 0.5 h, 1 h, 2 h, and 4 h. Thermolysin and α-chymotrypsin reactions were stopped by addition of 1% EDTA (Sigma-Aldrich, St. Louis, USA) and a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, USA), respectively.

Techniques: Incubation

Journal: Cell chemical biology

Article Title: Chemoselective restoration of para -azido-phenylalanine at multiple sites in proteins

doi: 10.1016/j.chembiol.2021.12.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Subsequently, Thermolysin (Promega, V4001) was added at a ratio of 1:10-1:20 and samples were incubated for 5 h at 80°C.

Techniques: Virus, Recombinant, Ubiquitin Proteomics, Software