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Gerstel GmbH
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UGO Basile S.R.L
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Elveflow Inc
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Linseis Messgerate
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Bio-Rad
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Bio-Rad
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Bio-Rad
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Revvity
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Revvity
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UGO Basile S.R.L
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Linseis Messgerate
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Gerstel GmbH
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Image Search Results
Journal: Experimental neurology
Article Title: Spinal cord injury in mice amplifies anxiety: A novel light-heat conflict test exposes increased salience of anxiety over heat.
doi: 10.1016/j.expneurol.2023.114382
Figure Lengend Snippet: Fig. 1. The TIDAL conflict test is a new assay for exploring the relative salience of anxiety vs. heat sensitivity. A. The TIDAL conflict test apparatus consists of two temperature-controlled plates linked by a center walkway. The mouse can freely move between an illuminated plate, which remains at an isothermic 31 ◦C, and a dark plate, which starts at 31 ◦C but incrementally increases to aversive temperatures. B. The thermal place preference (TPP) test is a control condition for the TIDAL test. Both chambers in TPP are illuminated; one plate increases in temperature, while the other plate remains at an isothermic 31 ◦C. C. Timeline of an TIDAL/TPP test, which takes ~40 min per mouse. The first 5 min with both plates at 31 ◦C is an acclimation period and is a control dark-light test. The TIDAL/TPP test begins with a second 31 ◦C session, then the heated plate increases in temperature incrementally, 1 ◦C every 5 min, from 39 to 44 ◦C. The isothermic plate remains at 31 ◦C throughout the test. D. Interpretation of potential TIDAL outcomes after SCI. At baseline (e.g., uninjured), mice are expected to decrease dark plate preference as its temperature increases. If SCI increases salience of heat (relative to dark-light), mice will leave the dark plate earlier – i.e., shifting the curve to the left. If SCI increases salience of anxiety (relative to heat), mice will prefer the dark plate to higher temperatures - i.e., shifting the curve to the right.
Article Snippet: Thermal Increments Dark-Light (TIDAL) Conflict Test: The TIDAL conflict apparatus is a modified thermal
Techniques: Control