Journal: The Journal of Clinical Hypertension

Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure

doi: 10.1111/jch.14784

Figure Lengend Snippet: Comparison of plasma HMGB1, RAGE, Th17, Treg, Th17/Treg, and NT‐proBNP levels.

Article Snippet: A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory.

Techniques: Comparison, Clinical Proteomics, Control

Journal: The Journal of Clinical Hypertension

Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure

doi: 10.1111/jch.14784

Figure Lengend Snippet: Flow cytometry results of Treg and Th17 in peripheral blood. Note : A and F are the results of PMA/Ionomycin unstimulated staining of IL‐17A (Th17 cells) and rat IgG2a isotype control (Treg cells), respectively. B and G are the Th17 and Treg detection results, respectively, of the healthy control group. C and H are the Th17 and Treg detection results, respectively, of the SPE group. D and I are the Th17 and Treg detection results, respectively, of the SPE+AHF group. E and J are the comparisons of the Th17 and Treg detection results, respectively, among the different groups, vs. healthy control group, a P < .05; vs. SPE group, b P < .05. SPE: severe preeclampsia; AHF: acute heart failure; Th17: T helper 17 cells; Treg: regulatory T cells.

Article Snippet: A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory.

Techniques: Flow Cytometry, Staining, Control

Journal: The Journal of Clinical Hypertension

Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure

doi: 10.1111/jch.14784

Figure Lengend Snippet: Table of independent variable collinearity diagnostic values.

Article Snippet: A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory.

Techniques: Diagnostic Assay

Journal: The Journal of Clinical Hypertension

Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure

doi: 10.1111/jch.14784

Figure Lengend Snippet: Critical results of random forest analysis characteristics of factors predicting AHF in patients with SPE. Note : HMGB1: high mobility group box 1 protein; RAGE: receptor for advanced glycation end products; Th17: T helper 17 cells; Treg: regulatory T cells; IL‐17: interleukin 17; IL‐6: interleukin 6; TGF‐β: transforming growth factor β; IL‐10: interleukin 10.

Article Snippet: A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory.

Techniques:

Journal: The Journal of Clinical Hypertension

Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure

doi: 10.1111/jch.14784

Figure Lengend Snippet: ROC curve parameters of various indicators for the diagnosis of AHF in SPE patients.

Article Snippet: A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory.

Techniques: Biomarker Discovery

Journal: The Journal of Clinical Hypertension

Article Title: Analysis of changes in high‐mobility group box 1, receptor for advanced glycation endproducts, and T helper 17/regulatory T balance in severe preeclampsia with acute heart failure

doi: 10.1111/jch.14784

Figure Lengend Snippet: ROC curve of each index in the diagnosis of AHF in SPE patients. Note : HMGB1: high mobility group box 1 protein; RAGE: receptor for advanced glycation end products; Th17: T helper 17 cells; Treg: regulatory T cells; IL‐17: interleukin 17; IL‐6: interleukin 6; TGF‐β: transforming growth factor β; IL‐10: interleukin 10.

Article Snippet: A Human Th17 staining kit (no: KTH117) and a human Treg staining kit (no: KTR101) were procured from Hangzhou Lianke Biotech Co., Ltd. RPMI‐1640 medium (catalog no.: 10491) was purchased from Beijing Solarbio Science & Technology Co., Ltd. HMGB1 (no.: MB‐3547A), RAGE (no.: MB‐1419A), IL‐17 (no.: MB‐0180A), IL‐6 (no.: MB‐0049A), TGF‐β (no.: MB‐3393A), IL‐10 (no.: MB‐3705A), and NT‐proBNP (no.: MB‐3940A) were detected using an enzyme‐linked immunosorbent assay (ELISA), and the respective ELISA kits were purchased from Jiangsu Meibiao Biotechnology Co., Ltd. A TDL‐40C low‐speed desktop large‐capacity centrifuge was purchased from Shanghai Anting Scientific Instrument Factory.

Techniques: Biomarker Discovery

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Comparison of Th17 cells, Treg cells, and Th17/Treg ratios between stroke patients and HCs.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques: Comparison

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Serum RBP4 was positively associated with a Th17/Treg imbalance. Association of serum RBP4 with Th17 cells (A) , Treg cells (B) , and the Th17/Treg ratio (C) in stroke patients. Association of serum RBP4 with Th17 cells (D) , Treg cells (E) , and the Th17/Treg ratio (F) in HCs.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques:

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Serum RBP4 and Th17/Treg imbalances were negatively associated with the MMSE score at enrollment. Association of serum RBP4 (A) , Th17 cells (B) , Treg cells (C) , and the Th17/Treg ratio (D) with MMSE score at enrollment in stroke patients.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques:

Journal: Frontiers in Neurology

Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline

doi: 10.3389/fneur.2023.1217979

Figure Lengend Snippet: Serum RBP4 and Th17/Treg imbalances were associated with the occurrence of cognitive impairment at enrollment. Association of serum RBP4 (A) , Th17 cells (B) , Treg cells (C) , and the Th17/Treg ratio (D) with cognitive impairment at enrollment in stroke patients.

Article Snippet: Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA).

Techniques: