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Image Search Results
Journal: Journal of Cell Science
Article Title: COMMD1 and PtdIns(4,5)P 2 interaction maintain ATP7B copper transporter trafficking fidelity in HepG2 cells
doi: 10.1242/jcs.231753
Figure Lengend Snippet: Colocalization of ATP7B with TGN46 or LAMP1. Cells were left untreated (basal medium), treated with 10 μM TTM (low copper) or treated with 10, 100 or 200 μM CuCl2. (A,B) Merged images show ATP7B in green, TGN46 (A) or LAMP1 (B) in magenta and the nucleus in blue; pixel overlap is shown in white. (C-H) 3D colocalization analysis produced M1, M2 and Pearson correlation coefficients for ATP7B and TGN46 (C-E) or LAMP1 (F-H). Values were calculated for each cell and plotted as mean±s.d., with contour plots for visualization of distribution. All data points are shown. Dunnett's method was used to compare treated and untreated cells (MEM); *P<0.05.
Article Snippet: Primary antibodies and concentrations used for immunofluorescence microscopy were as follows: 1:750 rabbit anti-ATP7B (Abcam, Cambridge, MA), 1:1000 mouse anti-LAMP1 (Developmental Studies Hybridoma Bank, Iowa City, Iowa), 1:300
Techniques: Produced
Journal: Journal of Cell Science
Article Title: COMMD1 and PtdIns(4,5)P 2 interaction maintain ATP7B copper transporter trafficking fidelity in HepG2 cells
doi: 10.1242/jcs.231753
Figure Lengend Snippet: Colocalization of ATP7B with TGN46 or LAMP1 in cells with reduced COMMD1 expression. Cells were transfected with COMMD1 (siCD1) or nontarget (control) siRNA and treated with TTM (low copper) or 200 μM CuCl2 and cycloheximide for the last hour. (A-C) 3D colocalization analysis of ATP7B with TGN46. (D-F) 3D colocalization analysis of ATP7B with LAMP1. The M1, M2 and Pearson coefficients were calculated for each cell and plotted as mean±s.d., with contour plots for visualization of distribution. All data points are shown. Student's t-test was used to compare COMMD1 knockdown with the control for each copper treatment; *P<0.05, **P<0.005.
Article Snippet: Primary antibodies and concentrations used for immunofluorescence microscopy were as follows: 1:750 rabbit anti-ATP7B (Abcam, Cambridge, MA), 1:1000 mouse anti-LAMP1 (Developmental Studies Hybridoma Bank, Iowa City, Iowa), 1:300
Techniques: Expressing, Transfection
Journal: Journal of Cell Science
Article Title: COMMD1 and PtdIns(4,5)P 2 interaction maintain ATP7B copper transporter trafficking fidelity in HepG2 cells
doi: 10.1242/jcs.231753
Figure Lengend Snippet: Colocalization of ATP7B with TGN46 or LAMP1 in cells with reduced COMMD1 expression. Cells were transfected with COMMD1 (siCD1) or nontarget (control) siRNA and treated with TTM (low copper) or 200 μM CuCl2 and cycloheximide with the addition of MG132 and chloroquine (CLQ) for the last hour. (A-C) 3D colocalization analysis of ATP7B with TGN46. (D-F) 3D colocalization analysis of ATP7B with LAMP1. The M1, M2 and Pearson coefficients were calculated for each cell and plotted as mean±s.d., with contour plots for visualization of distribution. All data points are shown. Student's t-test was used to compare COMMD1 knockdown with the control for each copper treatment; *P<0.05.
Article Snippet: Primary antibodies and concentrations used for immunofluorescence microscopy were as follows: 1:750 rabbit anti-ATP7B (Abcam, Cambridge, MA), 1:1000 mouse anti-LAMP1 (Developmental Studies Hybridoma Bank, Iowa City, Iowa), 1:300
Techniques: Expressing, Transfection
Journal: Journal of Cell Science
Article Title: COMMD1 and PtdIns(4,5)P 2 interaction maintain ATP7B copper transporter trafficking fidelity in HepG2 cells
doi: 10.1242/jcs.231753
Figure Lengend Snippet: Colocalization of ATP7B with TGN46 or LAMP1 in cells with reduced COMMD1 misexpression. Cells were transfected with one of three COMMD1 variants: GFP-COMMD1, GFP-COMMD1 T174M and GFP-COMMD1 K167/173E. They were then treated with TTM (low copper) or 200 μM CuCl2 for 9 h with cycloheximide added for the last hour. (A-C) 3D colocalization analysis of ATP7B with TGN46. (D-F) 3D colocalization analysis of ATP7B with LAMP1. The M1, M2 and Pearson coefficients were calculated for each cell and plotted as mean±s.d., with contour plots for visualization of distribution. All data points are shown. Dunnett's method was used to compare COMMD1 variants with the wild type; *P<0.05, **P<0.005.
Article Snippet: Primary antibodies and concentrations used for immunofluorescence microscopy were as follows: 1:750 rabbit anti-ATP7B (Abcam, Cambridge, MA), 1:1000 mouse anti-LAMP1 (Developmental Studies Hybridoma Bank, Iowa City, Iowa), 1:300
Techniques: Transfection
Journal: Journal of Cell Science
Article Title: COMMD1 and PtdIns(4,5)P 2 interaction maintain ATP7B copper transporter trafficking fidelity in HepG2 cells
doi: 10.1242/jcs.231753
Figure Lengend Snippet: Colocalization of ATP7B with TGN46 or LAMP1 in response to PtdIns(4,5)P2 modulation. HepG2 cells were transfected with the PH domain or ArfQ67L then treated with TTM (low copper) or 200 μM CuCl2 for 9 h with cycloheximide added for the last hour. (A-C) 3D colocalization analysis of ATP7B with TGN46. (D-F) 3D colocalization analysis of ATP7B with LAMP1. The M1, M2 and Pearson coefficients were calculated for each cell and plotted as mean±s.d., with contour plots for visualization of distribution. All data points are shown. Dunnett's method was used to compare cells expressing indicated proteins with control; *P<0.05, **P<0.005.
Article Snippet: Primary antibodies and concentrations used for immunofluorescence microscopy were as follows: 1:750 rabbit anti-ATP7B (Abcam, Cambridge, MA), 1:1000 mouse anti-LAMP1 (Developmental Studies Hybridoma Bank, Iowa City, Iowa), 1:300
Techniques: Transfection, Expressing