Journal: Scientific Reports

Article Title: Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells

doi: 10.1038/s41598-026-36706-9

Figure Lengend Snippet: Effect of RBE-Exos on the adhesion of NK-92 cells. ( A ) Growth status of NK-92 cells. NK cells treated with RBE-Exos lost the normal cluster growing. ( B ) CD11a protein levels. ( C ) CD18 protein levels. ( D ) CD54 protein levels. Western blot showed that the protein levels of CD11a, CD18 and, CD54 were obviously decreased in NK-92 cells treated with RBE-Exos. ( E ) Relative transcriptional levels of CD11a , CD18 , CD54 and, CD2 in NK-92 cells. qRT-PCR displayed that RBE-Exos downregulated the transcriptional levels of CD11a , CD18 , CD54 , but not CD2 . Data were presented as mean ± SEM for three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: The primary antibodies were as follows: rabbit anti-human CD63, BCL-2, BCL-XL, CD11a, β-actin (Abcam, USA), mouse anti-human HSP70 (Santa-Cruz, USA), rabbit anti-human CD18 (Proteintech, China), mouse anti-human CD54 (Proteintech, China).

Techniques: Western Blot, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: Cholangiocarcinoma derived exosomes attenuate the anti-tumor functions of NK cells

doi: 10.1038/s41598-026-36706-9

Figure Lengend Snippet: Cytotoxicity and expression of adhesion molecules in primary NK cells. ( A ) Cytotoxicity of primary NK cells from CCA patients and healthy people against target cells. LDH detection displayed the cytotoxicity of NK cells from CCA patients was significantly lower than that of NK cells healthy people. Data are presented as the mean ± SEM from n = 3 independent experiments. ( B ) Protein levels of CD18 in primary NK cells from CCA patients and healthy people. Data are presented as the mean ± SEM from n = 3 independent experiments. ( C ) Protein levels of CD54 in primary NK cells from CCA patients and healthy people. Data are presented as the mean ± SEM from n = 3 independent experiments. ** P < 0.01.

Article Snippet: The primary antibodies were as follows: rabbit anti-human CD63, BCL-2, BCL-XL, CD11a, β-actin (Abcam, USA), mouse anti-human HSP70 (Santa-Cruz, USA), rabbit anti-human CD18 (Proteintech, China), mouse anti-human CD54 (Proteintech, China).

Techniques: Expressing

Journal: Respiratory Research

Article Title: MiR-1307-5p enhances fibroblast transdifferentiation to exacerbate chronic obstructive pulmonary disease through regulating FBXL16/HIF1α axis

doi: 10.1186/s12931-024-03007-6

Figure Lengend Snippet: The reagents and antibodies used in this study

Article Snippet: Elisa kit , TGF-β (mouse) , Cusabio , CSB-E09785m.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Brain

Article Title: Distinct transcriptional changes distinguish efficient and poor remyelination in multiple sclerosis

doi: 10.1093/brain/awae414

Figure Lengend Snippet: Cellular expression of genes of interest in human multiple sclerosis brain tissue. ( A – D ) Immunofluorescent double-stained images of TGFβ1 ( A ), TGFβ2 ( B ), EGF ( C ) and BTC ( D ) with GFAP (astrocytes), HLA (microglia/macrophages) and SOX10 or Nogo-A (oligodendrocytes) in an active non-foamy lesion. Scale bars: 15 μm. Arrows indicate target + /cell marker + double-staining, arrowhead indicates target + /cell marker − double-staining, and asterisks indicate target − /cell marker + double-staining. ( E ) Percentage of cells per cell type expressing selected ligand and receptor pairs and average expression of each gene in each cell type (adapted from Absinta et al. ). OPC = oligodendrocyte precursor cell.

Article Snippet: Antibodies included TGFβ1 ( Ab215715 , 1:100; Abcam), TGFβ2 (116020, 1:100; NovoPro, Shanghai, China), EGF (Ab9695, 1:100; Abcam), BTC (AF-261, 1:100; R&D Systems) and BCAS1/NaBC1 (Sc-136342, 1:15 000; Santa Cruz).

Techniques: Expressing, Staining, Marker, Double Staining

Journal: Brain

Article Title: Distinct transcriptional changes distinguish efficient and poor remyelination in multiple sclerosis

doi: 10.1093/brain/awae414

Figure Lengend Snippet: Association between factors of interest with remyelinating multiple sclerosis tissue. ( A and B ) Histological quantification of targets of interest, TGFβ1, TGFβ2, EGF and BTC, in remyelinated lesions (RLs), active non-foamy lesions (ALs non-foamy), active foamy lesions (ALs foamy) and normal-appearing white matter (NAWM). Scale bars: 100 μm. Arrows indicate TGFβ1 + , TGFβ2 + , EGF + and BTC + cells, respectively. ( C ) Immunohistochemical staining of BCAS1. ( D – I ) Heat map of quantified proteins of interest and BCAS1 in RLs, ALs non-foamy, ALs foamy and NAWM shows a positive correlation between TGFβ1 and BCAS1 and between EGF and BTC. Statistics were performed using negative binomial generalized linear model or restricted maximum likelihood with Tukey’s post hoc test to compare values between groups. * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Antibodies included TGFβ1 ( Ab215715 , 1:100; Abcam), TGFβ2 (116020, 1:100; NovoPro, Shanghai, China), EGF (Ab9695, 1:100; Abcam), BTC (AF-261, 1:100; R&D Systems) and BCAS1/NaBC1 (Sc-136342, 1:15 000; Santa Cruz).

Techniques: Immunohistochemical staining, Staining