Journal: Life sciences

Article Title: Effects of chitinase-1 inhibitor in obesity-induced and -aggravated asthma in a murine model

doi: 10.1016/j.lfs.2023.122163

Figure Lengend Snippet: TGF- β (A), IL-1 β (B), IL-6 (C), TNF- α (D), IL-13 (E), and IL-4 (F) levels in lung homogenates Bar and line graphs present mean ± standard error. Cht, chitin; CPX, chitinase-1 inhibitor; OVA, ovalbumin * P < 0.05 between them analyzed by one-way ANOVA followed by post-hoc Bonferroni test.

Article Snippet: The concentrations of chitinase-1 (Fine biotech, EM0939, Wuhan, China), hydroxyproline (Biovision, K555–100, CA, USA), collagen-1 (Fine biotech, EM6446), interleukin (IL)-1 β (DY401), TNF- α (DY410), IL-4 (DY404), IL-6 (DY406), IL-13 (DY413), and TGF- β (DY1679) in lung homogenates were assessed using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, San Diego, USA) according to the manufacturer’s instructions.

Techniques:

Journal: Life sciences

Article Title: Effects of chitinase-1 inhibitor in obesity-induced and -aggravated asthma in a murine model

doi: 10.1016/j.lfs.2023.122163

Figure Lengend Snippet: Pathologic findings (H&E, PAS, and MT; all × 200 magnification) (A), quantitative fibrosis area (B), level of hydroxyproline in lung homogenates (C), and level of collagen-1 in lung homogenates (D). TGF- β (E), TNF- α (F), IL-1 β (G), IL-6 (H), IL-4 (I), and IL-13 (J) levels in lung homogenates Bar and line graphs present mean ± standard error. Cht, chitin; CPX, chitinase-1 inhibitor; HFD, high-fat diet; OVA, ovalbumin; H&E, hematoxylin and eosin; PAS, periodic acid-Schiff; MT, Masson’s trichrome * P < 0.05 between them analyzed by one-way ANOVA followed by post-hoc Bonferroni test.

Article Snippet: The concentrations of chitinase-1 (Fine biotech, EM0939, Wuhan, China), hydroxyproline (Biovision, K555–100, CA, USA), collagen-1 (Fine biotech, EM6446), interleukin (IL)-1 β (DY401), TNF- α (DY410), IL-4 (DY404), IL-6 (DY406), IL-13 (DY413), and TGF- β (DY1679) in lung homogenates were assessed using an enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, San Diego, USA) according to the manufacturer’s instructions.

Techniques:

Journal: Fibrogenesis & Tissue Repair

Article Title: L 59 TGF-β LAP degradation products serve as a promising blood biomarker for liver fibrogenesis in mice

doi: 10.1186/s13069-015-0034-9

Figure Lengend Snippet: Establishment of a sandwich ELISA for detecting L 59 LAP-DPs. a Schematic diagram of the ELISA for L 59 LAP-DPs. In the ELISA, L 59 LAP-DPs are first captured by coated L59 antibodies (Ab) and then sandwiched by biotin-conjugated anti-LAP antibodies (αLAP-Ab), which form a complex with strep-AP. For detection, an enzyme substrate is added and absorbance at 405 nm was measured. b , c Incubation time- and PLK concentration-dependent increases in the absorbance of the samples containing LAP incubated with PLK. The LAP (final 25 nM) was digested with 12.5 nM (open triangle), 25 nM (open reverse triangle), and 50 nM (open square) PLK, or without PLK (open circle) for 0–90 min, and then the samples were diluted by 1/50 and subjected to the L 59 LAP-DP ELISA ( b ). Also, 25 nM rhLAP was digested with 50 nM PLK (open square) or PLN (cross mark), or PLK in the presence of 5 μM camostat mesilate (open diamond) ( c ). All data were presented as mean ± SD from two different experiments ​*p-value <0.05, **p-value <0.01, ***p-value<0.001 obtained comparing to corresponding control values

Article Snippet: rhLAP β1 and anti-human LAP β1 mouse monoclonal antibodies were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Sandwich ELISA, Enzyme-linked Immunosorbent Assay, Incubation, Concentration Assay, Control

Journal: Fibrogenesis & Tissue Repair

Article Title: L 59 TGF-β LAP degradation products serve as a promising blood biomarker for liver fibrogenesis in mice

doi: 10.1186/s13069-015-0034-9

Figure Lengend Snippet: Correlation among L 59 LAP-DPs and active TGF-β in the culture medium, and intracellular signal transduction. a , c The ×9CAGA-Luc-transformed CCL64 cells were cultured in PLK-added CM derived from HEK293T cells overexpressing hLTGF-β1. After 6 h, the levels of active TGF-β1 and L 59 LAP-DPs were determined by respective ELISAs ( a ), and the extent of TGF-β signaling was measured by luciferase activity in CCL64 cells ( c ). b , d The scatterplots between the levels of L 59 LAP-DPs and active TGF-β shown in a ( b ) and between increases in L 59 LAP-DP levels and increases in luminescence from the values obtained compared to basal levels in the absence of PLK shown in c ( d ). A significant positive correlation was seen ( b ) * p-value <0.05, ***p-value <0.001 obtained comparing to 0

Article Snippet: rhLAP β1 and anti-human LAP β1 mouse monoclonal antibodies were purchased from R&D Systems (Minneapolis, MN, USA).

Techniques: Transduction, Transformation Assay, Cell Culture, Derivative Assay, Luciferase, Activity Assay

Journal: Journal of Ovarian Research

Article Title: Trophoblast cell surface antigen 2 (TROP2) from ascitic extracellular vesicles drives peritoneal metastasis of ovarian cancer by mesothelial-to-mesenchymal transition

doi: 10.1186/s13048-025-01845-6

Figure Lengend Snippet: Mesothelial-mesenchymal transition of peritoneal mesothelial cells induced by ascites-derived EVs. A TEM analysis of EVs. B NTA analysis of EVs. C Western blot analysis of EVs markers. D Dil-labeled EVs were incubated with HMrSV5 cells for 24 h and then observed under a fluorescence microscope (Dil in red and DAPI in blue). HMrSV5 cells were incubated with EVs derived from Cancer EVs and Normal EVs, respectively. The PBS group and the TGF-β1 (0.5 ng/mL) + IL-1β (2.5 ng/mL) group served as negative and positive controls, respectively. E - H . The protein levels of E-Cadherin, N-Cadherin and Vimentin in HMrSV5 cells incubated with Cancer EVs and Normal EVs. I - J . The effect of HMrSV5 cells incubated with Cancer EVs and Normal EVs migration analyzed by Transwell assay. K - M . Representative images depicting the adhesion of CMTPX-labelled SKOV3 (red) and OVCAR3 (red) cells to HMrSV5 cells (blue) are shown and adhered cells were quantified. Data are representative of at least three independent experiments and are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: D-Luciferin potassium salt was obtained from Yeasen (China), recombinant human TGF-β1 and human IL-1β were from R&D Systems (USA), and quinazoline (QNZ) was purchased from MCE (USA).

Techniques: Derivative Assay, Western Blot, Labeling, Incubation, Fluorescence, Microscopy, Migration, Transwell Assay