Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Dose response of LPS-induced lethality and TNF-α activity in serum in rabbits primed with TSST-1

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Activity Assay

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Time course of LPS-induced TNF-α in serum in Dutch belted rabbits primed with TSST-1. Groups of three rabbits were injected with 10.0 ng (i.v.) of TSST-1 (primed)/kg or an equivalent volume of PBS (unprimed). All rabbits were injected with LPS (10 μg/kg [i.v.]) 12 h later. Control rabbits injected with 5 μg of TSST-1 and PBS/kg 12 h later did not develop detectable levels of TNF-α. (∗, P ≤ 0.05).

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Injection, Control

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Direct comparison of LPS-induced TNF-α in serum in mice and rabbits primed with TSST-1. Groups of three BALB/c-AnNCr mice and three Dutch belted rabbits were injected with TSST-1 at doses of 200 μg/kg (i.p.) or 10.0 ng/kg (i.v.), respectively. After 12 h, mice and rabbits were injected with LPS at doses of 400 μg/kg (i.p.) or 10 μg/kg (i.v.), respectively. TNF-α activity in serum was measured at 2 h postinjection of LPS in both species. The levels of TNF-α due to LPS alone were calculated from previous time course studies with the same mice and rabbits. Injection of TSST-1 alone did not induce detectable levels of TNF-α in serum in control animals. The mean peak TNF-α level induced by TSST-1 plus LPS in mice did not significantly differ from that measured in rabbits.

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Comparison, Injection, Activity Assay, Control