Journal: Cell metabolism

Article Title: CD38-NAD + Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response

doi: 10.1016/j.cmet.2017.10.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mycoplasma testing was done in Mehrotra lab. N/A Experimental Models: Organisms/Strains C57BL/6 Jackson Laboratory Stock# 000664 C57BL/6-Tg(HLA-A2.1)1Enge/J Jackson Laboratory Stock# 003475 B6.129S7- Rag1 tm1Mom /J Jackson Laboratory Stock# 002216 B6.129P2- Cd38 tm1Lnd /J Jackson Laboratory Stock# 003727 B6;129- Sirt1 tm1Ygu /J Jackson Laboratory Stock# 008041 Tg(Cd4-cre)1Cwi/BfluJ Jackson Laboratory Stock# 017336 B6.SJL- Ptprc a Pepc b /BoyJ Jackson Laboratory Stock# 002014 B6.129S7- Ifng tm1Ts /J Jackson Laboratory Stock# 002287 B6.Cg- Rag1 tm1Mom Tyrp1 B-w Tg(Tcra,Tcrb)9Rest/J Jackson Laboratory Stock# 008684 B6.Cg- Thy1 a /Cy Tg(TcraTcrb)8Rest/J Jackson Laboratory Stock# 005023 C57BL/6-Tg(TRAMP)8247Ng/J Jackson Laboratory Stock# 003135 IFNγ Thy1.1 knockin Casey T. Weaver, University of Alabama at Birmingham (UAB) ( Harrington et al., 2008 ) Foxo1 fl/fl Lck cre Melanie Gubbels Bupp, Randolph-Macon College, VA ( Gubbels Bupp et al., 2009) TCR-I Jennifer Wu, MUSC ( Staveley-O’Carroll et al., 2003) Oligonucleotides Primers for qPCR, see Table S4 Recombinant DNA MSGV1-TRP-1 vector ( Kerkar et al., 2011 ) N/A LV-TCR/sr39TK-GFP vector ( Koya et al., 2010 ) N/A Software and Algorithms FlowJo 10.2 TreeStar, OR https://www.flowjo.com/solutions/flowjo/downloads/ Prism 5 GraphPad https://www.graphpad.com/scientiflc-software/prism/ Agilent Seahorse Wave 2.4 Agilent http://www.agilent.com/en-us/products/cell-analysis-(seahorse )/seahorse-wave-software CFX Manager 3.1 Biorad http://www.bio-rad.com/en-us/sku/soft-cfx-31-patch-cfx-manager-software-v3-1-upgrade GenomeStudio software Illumina https://www.illumina.com/techniques/microarrays/array-data-analysis-experimental-design/genomestudio.html PIMENTo ( Sásik et al., 2004 ) https://github.com/MUSC-CGM/PIMENTo ToppGene Suite ( Chen et al., 2009 ) https://toppgene.cchmc.org/ Venny This paper http://bioinfogp.cnb.csic.es/tools/venny/index.html Open in a separate window KEY RESOURCES TABLE Th1/17 cells with effector and stemness features exhibit durable tumor control High glutaminolysis of Th1/17 cell regulates its viability and anti-tumor response NAD + -Sirt1-Foxo1 axis is central to the anti-tumor phenotype of Th1/17 cells Targeting NADase CD38 on T cells increases NAD + levels and controls tumor growth

Techniques: Recombinant, Staining, Cell Isolation, Synthesized, Lysis, Extraction, Cell Based Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Immunoprecipitation, cDNA Synthesis, SYBR Green Assay, Microarray, Knock-In, Plasmid Preparation, Software

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Dose response of LPS-induced lethality and TNF-α activity in serum in rabbits primed with TSST-1

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Activity Assay

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Time course of LPS-induced TNF-α in serum in Dutch belted rabbits primed with TSST-1. Groups of three rabbits were injected with 10.0 ng (i.v.) of TSST-1 (primed)/kg or an equivalent volume of PBS (unprimed). All rabbits were injected with LPS (10 μg/kg [i.v.]) 12 h later. Control rabbits injected with 5 μg of TSST-1 and PBS/kg 12 h later did not develop detectable levels of TNF-α. (∗, P ≤ 0.05).

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Injection, Control

Journal:

Article Title: Comparative Analysis of Lipopolysaccharide-Induced Tumor Necrosis Factor Alpha Activity in Serum and Lethality in Mice and Rabbits Pretreated with the Staphylococcal Superantigen Toxic Shock Syndrome Toxin 1

doi: 10.1128/IAI.69.11.7169-7172.2001

Figure Lengend Snippet: Direct comparison of LPS-induced TNF-α in serum in mice and rabbits primed with TSST-1. Groups of three BALB/c-AnNCr mice and three Dutch belted rabbits were injected with TSST-1 at doses of 200 μg/kg (i.p.) or 10.0 ng/kg (i.v.), respectively. After 12 h, mice and rabbits were injected with LPS at doses of 400 μg/kg (i.p.) or 10 μg/kg (i.v.), respectively. TNF-α activity in serum was measured at 2 h postinjection of LPS in both species. The levels of TNF-α due to LPS alone were calculated from previous time course studies with the same mice and rabbits. Injection of TSST-1 alone did not induce detectable levels of TNF-α in serum in control animals. The mean peak TNF-α level induced by TSST-1 plus LPS in mice did not significantly differ from that measured in rabbits.

Article Snippet: When measured in the bioassay for TNF-α, the specific activity of murine recombinant TNF-α (R & D systems) was 2.56 × 10 8 U/mg, while that of rabbit TNF-α in conditioned medium (Pharmingen) was 3.72 × 10 8 U/mg.

Techniques: Comparison, Injection, Activity Assay, Control