Journal: Journal of Cellular and Molecular Medicine

Article Title: Electroacupuncture Pretreatment Ameliorates Perioperative Neurocognitive Disorder in Aged Mice by Inhibiting Ferroptosis Through the SIRT1 / NRF2 / GPX4 Pathway

doi: 10.1111/jcmm.71021

Figure Lengend Snippet: SIRT1/NRF2/GPX4 pathway is involved in hippocampal ferroptosis in aged mice. (A) WB images and quantification analysis of SIRT1, NRF2 and GPX4 in the hippocampus of aged mice. (B) WB images and quantification analysis of SLC7A11, TFR1, IRP2 and ferritin in the hippocampus of aged mice ( n = 3 per group). (C) qRT‐PCR expression of SIRT1, NRF2, GPX4, SLC7A11, TFR1, IRP2 and ferritin mRNA in the hippocampus of aged mice ( n = 3 per group). Values are presented as mean ± SEM. ** p < 0.01 compared with the C group; # p < 0.05 and ## p < 0.01 compared with the M group; + p < 0.05 and ++ p < 0.01 and compared with the EX group.

Article Snippet: The membrane was then incubated overnight at 4°C with primary antibodies: SIRT1 (1:850; Lot‐19G10A10; BOSTER), NRF2 (1:1500; Cat#YT3189; Immunoway), iron regulatory protein 2 (IRP2) (1:3000; Cat#YN3307; Immunoway), transferrin receptor 1 (TFR1) (1:750; LotNo‐23BP65E1; BOSTER), GPX4 (1:1500; Cat#YN3047; Immunoway), ferritin (1:3000; Cat#YT1692; Immunoway) and SLC7A11 (1:2000; Cat#YT8130; Immunoway).

Techniques: Quantitative RT-PCR, Expressing

Journal: The Journal of Immunology

Article Title: In This Issue

doi: 10.4049/jimmunol.1590014

Figure Lengend Snippet: FIGURE 1. Impact of CD71+ erythroid cells on inflammatory-mediated mortality in neonates. (A) Survival after adoptive transfer with endotoxin ex- posure. Neonatal mice received 3 3 106 highly enriched neonatal CD71+ erythroid (Ter119+) cells via i.p. injection 30 min prior to endotoxin challenge (LD40). (B) Survival after adoptive transfer with polymicrobial sepsis. Neonatal mice received 5 3 106 highly enriched neonatal splenic CD71+ erythroid cells, B220+ cells, or PBS via i.p. injection 30 min prior to sepsis (LD60). (C) Gross spleen appearance and splenic CD71+ erythroid cell representation by FACS in previously healthy neonatal mice following anti-CD71 or isotype Ab treatment. Data are mean + SD. (D) Impact of anti-CD71 treatment or isotype-control Ab on sepsis survival following low (top; LD20) and high (bottom; LD60) mortality challenges. *p , 0.05, t test.

Article Snippet: Resident CD71+ erythroid splenocytes were diminished via i.p. administration of anti-CD71 mAb (150 mg/mouse; Bio X Cell, clone R17 217.1.3/TIB-219) daily for 2 d. This dose was chosen based on a previous report and our empiric results (8).

Techniques: Adoptive Transfer Assay, Injection, Control

Journal: The Journal of Immunology

Article Title: In This Issue

doi: 10.4049/jimmunol.1590014

Figure Lengend Snippet: FIGURE 3. Anti-CD71 treatment is associated with enhanced bacterial clearance due to immune priming. (A) Splenic bacterial load 24 h after nonlethal sepsis challenge (0.6 mg of cecal slurry/g body weight given 24 h after the last Ab dose) in CD71-depleted neonates that received either 5 3 106 highly enriched neonatal splenic CD71+ erythroid cells by adoptive transfer or no cells. (B) CD71 expression in healthy neonatal ileum (original magnification 3200). (C) Isotype Ab–treated neonatal ileum and anti-CD71–treated ileum 24 h after the last dose of Ab (original magnification 3200). (D) Reduction in CD71 MFI in ileum of anti-CD71–treated and isotype control–treated neonates 24 h after the last dose of Ab. (E) CFU recovered from peritoneal washes of healthy neonates 24 h after last dose of isotype Ab or anti-CD71 treatment alone. (F) Splenic bacterial load 24 h after nonlethal sepsis challenge (0.6 mg of cecal slurry/g body weight given 24 h after the last Ab dose) among neonates that received treatment with anti-CD71 Ab or isotype Ab or priming with a single dose of LPS (1 mg/g, 24 h prior to challenge). *p , 0.05, ANOVA.

Article Snippet: Resident CD71+ erythroid splenocytes were diminished via i.p. administration of anti-CD71 mAb (150 mg/mouse; Bio X Cell, clone R17 217.1.3/TIB-219) daily for 2 d. This dose was chosen based on a previous report and our empiric results (8).

Techniques: Adoptive Transfer Assay, Expressing, Control

Journal: The Journal of Immunology

Article Title: In This Issue

doi: 10.4049/jimmunol.1590014

Figure Lengend Snippet: FIGURE 4. CD71+CD235a+ cells are prominent in human neonatal cord blood and are predominantly enucleated reticulocytes. (A) Representative FACS plots for post-Ficoll mono- nuclear fraction of healthy adult peripheral blood, healthy term neonate cord blood, healthy term neonate cord blood after hypotonic lysis, enrich- ment of neonatal cord blood CD71+235a+ cells, H&E stain of enriched CD71+CD235a+ cells (top), methylene blue stain of enriched CD71+

Article Snippet: Resident CD71+ erythroid splenocytes were diminished via i.p. administration of anti-CD71 mAb (150 mg/mouse; Bio X Cell, clone R17 217.1.3/TIB-219) daily for 2 d. This dose was chosen based on a previous report and our empiric results (8).

Techniques: Lysis, Staining