Journal: PLOS ONE

Article Title: Roles of adipose-derived stem cells and derived exosomes in therapeutic applications to testicular injury caused by cisplatin

doi: 10.1371/journal.pone.0297076

Figure Lengend Snippet: A: Showed a significant decrease in the layer of germ cells and a sparse arrangement of the seminiferous tubules in the testicular tissue of the model group compared to the control group. B: Showed a significant decrease in the expression level of c-Kit in the model group compared to the control group. C: Showed a significant increase in the levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), a significant decrease in the level of testosterone (T).

Article Snippet: ELISA kits were used to identify the levels of follicle-stimulating hormone (FSH, E-EL-RO391c, abscience) and luteinizing hormone (LH, E-EL-RO026c, Elabscience), testosterone (T, E-OSEL-R0003, ascience), malondialdehyde (MDA, E-EL-0060c,Elabscience).

Techniques: Control, Expressing

Journal: Cancer Prevention Research

Article Title: Endocrine-Immune-Paracrine Interactions in Prostate Cells as Targeted by Phytomedicines

doi: 10.1158/1940-6207.capr-08-0062

Figure Lengend Snippet: Fig. 3. Effects of DHEA, TGFβ1, and IL-6 on testosterone secretion in cocultured LAPC-4/6S cells. LAPC-4 cells were seeded in treatment media in triplicate onto 30-mm inserts coated with a film of Matrigel at a density of 5 × 105 per insert. Stromal cells (6S) were seeded in triplicate at 1 × 105 per well in 24-well plates. TGFβ1 was added to stromal cultures on the same day at 40 pmol/L to stimulate a reactive stromal phenotype. Cocultures were combined after 2 d while monocultures remained separated; hormones were added; and cells allowed to coculture for 3 d. Media containing hormones were replaced and allowed to condition for 48 h. Conditioned media were assayed for testosterone by ELISA. Each original triplicate experimental sample was assayed in duplicate. Testosterone values were normalized to cell numbers as determined by the modified MTT assay. Columns, mean from three separate experiments; bars, SE. *, P = 0.05; **, P = 0.01, within monoculture or coculture. +, P = 0.05; +++, P < 0.001, between monoculture and coculture.

Article Snippet: Total testosterone was also measured with an ELISA kit (ALPCO).

Techniques: Enzyme-linked Immunosorbent Assay, Modification, MTT Assay

Journal: Cancer Prevention Research

Article Title: Endocrine-Immune-Paracrine Interactions in Prostate Cells as Targeted by Phytomedicines

doi: 10.1158/1940-6207.capr-08-0062

Figure Lengend Snippet: Fig. 4. Red clover effects on TGFβ1 + DHEA–stimulated LAPC-4/6S cocultures. A, LAPC-4 PSA production. The same experimental procedure was used as in the PSA ELISA experiment depicted in Fig. 2A, but in addition to hormone treatments of 100 nmol/L DHEA +/−40 pmol/L TGFβ1, cells were also treated with DHEA/TGFβ1 + 100 nmol/L red clover (RC) isoflavones; DHEA/ TGFβ1 + red clover plus 100 nmol/L ICI 182,780 (ICI; estrogen receptor antagonist); DHEA/TGFβ1 + 100 nmol/L E2; TGFβ1 alone; or 10 nmol/L R1881. Columns, average from three separate experiments; bars, SE. B, LAPC-4 PSA gene expression. LAPC-4 cells were plated in triplicate in monoculture and in coculture with 6S stromal cells, as described in Fig. 2B. Stromal cells were pretreated with 40 pmol/L TGFβ1 for 3 d, then cultures were combined and treated with 100 nmol/L DHEA +/−40 pmol/L TGFβ1; DHEA/TGFβ1 + 100 nmol/L red clover isoflavones; DHEA/TGFβ1 + red clover + 1 μmol/L ICI 182,780 (estrogen receptor antagonist); DHEA/TGFβ1 + 100 nmol/L E2; or 10 nmol/L R1881 for 48 h. RNA was extracted and cDNA was reverse transcribed and probed by real-time PCR for PSA expression, standardized to ribosomal phosphoprotein PO expression. Columns, mean from three experiments; bars, SE. C, stromal testosterone secretion in cocultured LAPC-4/ 6S cells. Testosterone concentrations were determined in conditioned media from stromal cell monocultures, compared with cocultures from the same experiments illustrated in Fig. 3. Hormone treatments include 100 nmol/L DHEA +/−40 pmol/L TGFβ1; DHEA/TGFβ1 + 100 nmol/L red clover isoflavones; DHEA/TGFβ1 + red clover plus 100 nmol/L ICI 182,780; DHEA/TGFβ1 + 100 nmol/L E2; TGFβ1 alone; or 10 nmol/L R1881. Columns, mean from three experiments; bars, SE. *, P = 0.05; **, P = 0.01; +, P = 0.05; ++, P = 0.01; +++, P = 0.001; ⧫, P = 0.05, compared with DHEA + TGFβ1 alone.

Article Snippet: Total testosterone was also measured with an ELISA kit (ALPCO).

Techniques: Enzyme-linked Immunosorbent Assay, Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing

Journal: Cancer Prevention Research

Article Title: Endocrine-Immune-Paracrine Interactions in Prostate Cells as Targeted by Phytomedicines

doi: 10.1158/1940-6207.capr-08-0062

Figure Lengend Snippet: Fig. 5. Dose-responsive effects of red clover isoflavones to inhibit DHEA + TGFβ1–induced PSA expression in LAPC-4 cell monocultures and cocultures and testosterone metabolism in 6S stromal cell monocultures and cocultures. A, the same experimental procedure for PSA ELISA was used as in Figs. 2 and 4, but in addition to hormone treatments of 100 nmol/L DHEA +/−40 pmol/L TGFβ1 and 10 nmol/L R1881, cells were also treated with DHEA + TGFβ1 + red clover isoflavones at 10, 30, 100, or 300 nmol/L. B, testosterone concentrations were determined in conditioned media from stromal cell monocultures, compared with cocultures from the same experiments represented in A. Hormone treatments included 100 nmol/L DHEA +/−40 pmol/L TGFβ1; DHEA/TGFβ1 + red clover isoflavones at 10, 30, 100, or 300 nmol/L; and 10 nmol/L R1881. Columns, mean from three experiments; bars, SE. *, P = 0.05; **, P = 0.01; ***, P = 0.001; +, P = 0.05; ++, P = 0.01; +++, P = 0.001; ⧫, P = 0.05, compared with DHEA + TGFβ1 alone.

Article Snippet: Total testosterone was also measured with an ELISA kit (ALPCO).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay