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Journal: bioRxiv
Article Title: Aeromonas adhesins facilitate kin and non-kin attachment to enable T6SS-mediated antagonism in liquid
doi: 10.64898/2026.01.27.701733
Figure Lengend Snippet: LiQuoR-based viability measurements (RLU) of E. coli prey before (t = 0 h) and after (t = 4 h) co-incubation with wild-type (WT) or T6SS − (Δ hcp1 /Δ tssM2 ) Vibrio coralliilyticus ATCC BAA-450 attacker strains (A) , or with WT or T6SS − (Δ hcp1 /Δ hcp2 ) Vibrio parahaemolyticus RIMD 2210633 attacker strains (B) at a 4:1 (attacker:prey) ratio in LB or MLB media (1% or 3% [w/v] NaCl, respectively) with or without 1 mM CaCl 2 at 30 ° C. Data are shown as the mean ± SD; n = 3 samples. The statistical significance between samples at the 4 h time point was calculated using an unpaired, two-tailed Student’s t test. ns, P > 0.05. The experiments were repeated three times with similar results. Results from a representative experiment are shown.
Article Snippet: To investigate the possibility of other marine bacteria intoxicating competitors in liquid media using T6SS, we employed two Vibrio species whose T6SSs we have previously characterized: (1) the
Techniques: Incubation, Two Tailed Test