Journal: Frontiers in Physiology

Article Title: Impact of Bilateral Sympathetic Stellate Ganglionectomy on TGF-β1 Signaling Pathway in Rats With Chronic Volume Overload

doi: 10.3389/fphys.2020.00375

Figure Lengend Snippet: Expressions of norepinephrine (NE) and acetylcholine (ACh) in the plasma, right ventricle (RV), and left ventricle (LV) by ELISA. (A) The expression of NE was significantly increased in the abdominal aorta-cava fistula (AV) group and decreased in the bilateral sympathetic stellate ganglionectomy after abdominal aorta-cava fistula (AD) group in the plasma, RV, and LV. (B) The expression of ACh was significantly decreased in the AV group and increased in the AD group in the RV (* P < 0.05, ** P < 0.01) (three replicate experiments were performed).

Article Snippet: Acetylcholine (ACh) concentrations of the plasma, RV, and LV were determined with the ELISA kit (BioVision, E4454-100, Milpitas, CA, United States) following the manufacturer’s instructions.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Arthritis Research & Therapy

Article Title: Elevated serum immunoglobulin level predicts high risk of 1-year recurrence in patients with Takayasu arteritis

doi: 10.1186/s13075-023-03016-8

Figure Lengend Snippet: CD138 expressions in the aortic wall of TAK and control group. A CD138 staining in the aortic wall of TAK patients and control group (scale bars, 100 μm; n = 3 in control group, n = 4 in TAK group). B The quantification of CD138 staining in the two groups ( n = 3 in control group, n = 4 in TAK group)

Article Snippet: Then, they were stained with primary antibody for CD138 (Item No.ab46506, Abcam, Cambridge, CB2 0AX, UK) overnight at 4 °C and secondary antibody for 0.5–1 h at room temperature and detected with 3,3′-diaminobenzidine.

Techniques: Staining

Journal:

Article Title:

doi:

Figure Lengend Snippet: Endogenous DMT1 is released in EVs from mouse gut explants. ( A ) Endogenous DMT1 is released in EVs by mouse gut explants under normal and high iron conditions. CD26 is used as a loading control. ( B ) Densitometry quantification of DMT1 release in EVs from the gut normalized against CD26 shows a trend for increase in the amount of DMT1 released under high iron conditions. Data are mean±s.e.m.; n =4 animals per group. ( C ) Quantitative PCR (Q-PCR) shows a ~8.5-fold increase in the expression of Arrdc4 in the duodenum of mice fed a high iron diet compared with normal iron diet. The TATA box binding protein was used as the reference gene. Data are mean±s.e.m., n =3–4, ** P <0.01. ( D ) DMT1 is released in gut EVs from WT and Arrdc1 −/− mice. ( E ) Densitometry quantification of DMT1 release in EVs from the gut normalized against CD26 shows that the levels of DMT1 released in gut EVs is not changed in Arrdc1 −/− compared with WT mice. ( F ) The protein concentration of EVs released from Arrdc1 −/− gut EVs is the same as from WT gut EVs. Data are mean±s.e.m.; n =4 animals per group. ( G ) DMT1 is released in gut EVs from WT and Arrdc4 −/− mice. ( H ) The levels of DMT1 released in gut EVs is not changed in Arrdc4 −/− compared with WT mice. ( I ) The protein concentration of EVs released from Arrdc4 −/− gut EVs is significantly reduced compared with WT gut EVs. Data are mean±s.e.m.; n =4 animals per group. ** P <0.01.

Article Snippet: Sources of commercial antibodies were as follows: rat monoclonal anti-HA (clone 3F10) and mouse monoclonal anti-c-myc (clone 9E10) from Roche Diagnostics (Indianapolis, IN, USA); rabbit polyclonal anti-GFP (clone ab290), mouse monoclonal anti-CD63 (MEM 259), goat polyclonal Myc (ab9132), rabbit polyclonal anti-CD26 (ab28340) and rabbit anti-LAMP1 from Abcam (Cambridge, MA, USA); mouse anti-flotillin-2 antibody (clone 29), mouse anti-γ adaptin, mouse anti-GM130 from BD Biosciences (San Jose, CA, USA); mouse monoclonal anti-β-actin (clone AC-15), rat monoclonal anti-Flag (clone 6F7), mouse monoclonal anti-Flag (clone M2) and rabbit polyclonal anti-Tsg101 (Sigma-Aldrich, St Louis, MO, USA), goat polyclonal anti-GFP from Rockland Immunochemicals (Limerick, PA, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Binding Assay, Protein Concentration