Journal: American Journal of Human Genetics

Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development

doi: 10.1016/j.ajhg.2017.07.010

Figure Lengend Snippet: Mutations in TBC1D23 Cause PCH

Article Snippet: Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 .

Techniques:

Journal: American Journal of Human Genetics

Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development

doi: 10.1016/j.ajhg.2017.07.010

Figure Lengend Snippet: Representation of the TBC1D23 Polypeptide

Article Snippet: Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 .

Techniques:

Journal: American Journal of Human Genetics

Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development

doi: 10.1016/j.ajhg.2017.07.010

Figure Lengend Snippet: Clinical and Radiological Features of Individuals with TBC1D23 Mutations

Article Snippet: Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 .

Techniques: Expressing

Journal: American Journal of Human Genetics

Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development

doi: 10.1016/j.ajhg.2017.07.010

Figure Lengend Snippet: Effects of TBC1D23 Downregulation on Neuronal Migration and Positioning

Article Snippet: Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 .

Techniques: Migration

Journal: American Journal of Human Genetics

Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development

doi: 10.1016/j.ajhg.2017.07.010

Figure Lengend Snippet: TBC1D23 Truncating Mutation Alters Intracellular Transport

Article Snippet: Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 .

Techniques: Mutagenesis

Journal: American Journal of Human Genetics

Article Title: Homozygous Truncating Variants in TBC1D23 Cause Pontocerebellar Hypoplasia and Alter Cortical Development

doi: 10.1016/j.ajhg.2017.07.010

Figure Lengend Snippet: TBC1D23 Downregulation Alters Neurite Outgrowth

Article Snippet: Three different 29-mer shRNA sequences targeting mouse Tbc1d23 (GenBank: {"type":"entrez-nucleotide","attrs":{"text":"NM_026254","term_id":"27754078","term_text":"NM_026254"}} NM_026254 ) commercially designed and provided by Origene were cloned in the psiStrike vector under the control of a U6 promoter: shRNA-1, targeting the sequence 5′-AGAATCCATCTGAGTTTGCACAGTCAGT-3′, specific for both mouse and human TBC1D23 ; shRNA-2, targeting the sequence 5′-TTACATCCAGTCTCGGCAAGCACTAAATT-3′ and specific for mouse Tbc1d23 ; and shRNA-3, targeting the sequence 5′-TGTTAGCATTGCCAGTGGAGGATTTATGG-3′, also specific for mouse Tbc1d23 .

Techniques: