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Image Search Results
Journal: Addiction Biology
Article Title: Chronic Intermittent Ethanol and Withdrawal Suppress Evoked and Spontaneous GABA Release Onto Distinct Populations of Basolateral Amygdala Principal Neurons
doi: 10.1111/adb.70080
Figure Lengend Snippet: Compared to males, females have less colocalization between vGAT and PV expression associated with BLA NAc neurons. (A) Representative images of vGAT (purple), PV (green), tdTomato (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.
Article Snippet: The rats received a bilateral microinjection of an AAVrg encoding GFP (AAVrg‐CAG‐GFP; Addgene 37 825) or
Techniques: Expressing, Staining