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Addgene inc tdtomato
Compared to males, females have less colocalization between vGAT and PV expression associated with <t>BLA</t> <t>NAc</t> neurons. (A) Representative images of vGAT (purple), PV (green), <t>tdTomato</t> (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.
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Bio-Rad whole gel eluter
Compared to males, females have less colocalization between vGAT and PV expression associated with <t>BLA</t> <t>NAc</t> neurons. (A) Representative images of vGAT (purple), PV (green), <t>tdTomato</t> (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.
Whole Gel Eluter, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad cdna templates
Compared to males, females have less colocalization between vGAT and PV expression associated with <t>BLA</t> <t>NAc</t> neurons. (A) Representative images of vGAT (purple), PV (green), <t>tdTomato</t> (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.
Cdna Templates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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OriGene human cd38
Compared to males, females have less colocalization between vGAT and PV expression associated with <t>BLA</t> <t>NAc</t> neurons. (A) Representative images of vGAT (purple), PV (green), <t>tdTomato</t> (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.
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OriGene mir29a
Compared to males, females have less colocalization between vGAT and PV expression associated with <t>BLA</t> <t>NAc</t> neurons. (A) Representative images of vGAT (purple), PV (green), <t>tdTomato</t> (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.
Mir29a, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Compared to males, females have less colocalization between vGAT and PV expression associated with BLA NAc neurons. (A) Representative images of vGAT (purple), PV (green), tdTomato (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.

Journal: Addiction Biology

Article Title: Chronic Intermittent Ethanol and Withdrawal Suppress Evoked and Spontaneous GABA Release Onto Distinct Populations of Basolateral Amygdala Principal Neurons

doi: 10.1111/adb.70080

Figure Lengend Snippet: Compared to males, females have less colocalization between vGAT and PV expression associated with BLA NAc neurons. (A) Representative images of vGAT (purple), PV (green), tdTomato (red) and overlap (‘Merged’) at 40× magnification in males. ROIs drawn around tdTomato + soma (BLA NAC neurons) are indicated across each panel and enlarged for colocalization comparisons (far right). Yellow arrows indicate vGAT + /PV + puncta within tdTomato + ROIs. Blue arrows indicate apparent vGAT + /PV − puncta within these regions. (B) Manders overlap values for vGAT and PV staining in air‐ and CIE/WD‐exposed male and female BLA NAC neurons. For M1 (B 1 ), two‐way ANOVA across sex and treatment factors revealed no interaction but significantly less overlap of vGAT + puncta with PV + puncta in females relative to males regardless of treatment; sex: p < 0.0001, F (1, 28) = 35.92, n = 8 slices per condition from three different animals. For M2, there were no significant interaction or main effects of either sex or CIE/WD for the colocalization of PV + puncta with vGAT + puncta.

Article Snippet: The rats received a bilateral microinjection of an AAVrg encoding GFP (AAVrg‐CAG‐GFP; Addgene 37 825) or tdTomato (AAVrg‐CAG‐tdTomato; Addgene 59 642) into either the NAC or the BNST using the following coordinates (millimetres in relation to bregma): NAC (AP +1.80, ML ± 1.50, DV 6.72) and BNST (AP −0.36, ML ± 0.80, DV 6.45).

Techniques: Expressing, Staining