Journal:

Article Title: Replication of Herpes Simplex Virus 1 Depends on the ? 1 34.5 Functions That Facilitate Virus Response to Interferon and Egress in the Different Stages of Productive Infection

doi: 10.1128/JVI.78.14.7653-7666.2004

Figure Lengend Snippet: Cellular distribution of virus particles in MEF 3T6 cells. Confluent monolayers of MEF 3T6 cells were infected with HSV-1(F), R3616, or JL0253R at 0.5 PFU per cell. At 24 h postinfection, cells were first fixed in 4% glutaraldehyde in 100 mM phosphate buffer (pH 6.8 to 7.2) and then fixed in 1% osmium tetroxide. Cells were dehydrated in ethanol, embedded in LX112 resin, and stained with uranyl acetate and lead citrate. Thin sections were prepared and viewed with a Joel 1220 transmission electron microscope at 80 kV. Images were captured with a Gatan digital CCD camera. (A) HSV-1(F)-infected MEF 3T6 cells. (B) R3616-infected MEF 3T6 cells. (C) JL0253R-infected MEF 3T6 cells. Scale bars are shown in each panel. Abbreviations: Nuc, nuclear region; Cyt, cytoplasm.

Article Snippet: Images were taken at various magnifications with a digital charge-coupled device (CCD) camera (Software digital micrograph; Gatan Inc.).

Techniques: Infection, Staining, Transmission Assay, Microscopy

Journal:

Article Title: Replication of Herpes Simplex Virus 1 Depends on the ? 1 34.5 Functions That Facilitate Virus Response to Interferon and Egress in the Different Stages of Productive Infection

doi: 10.1128/JVI.78.14.7653-7666.2004

Figure Lengend Snippet: Intracellular distribution of virions in cells infected with γ134.5 mutants with deletions in the amino-terminal domain. Confluent monolayers of MEF 3T6 cells in 35-mm dishes were infected with the indicated viruses at 0.5 PFU per cell. At 24 h postinfection, cells were harvested and processed for electron microscopic analysis as described in Materials and Methods. Digital images were taken at various magnifications with a Gatan digital CCD camera. R4002-infected MEF 3T6 cells (A), R931-infected MEF 3T6 cells (B), R908-infected MEF 3T6 cells (C), and R909-infected MEF 3T6 cells (D) are shown. Scale bars are shown in each picture. Nuc, nuclear region; Cyt, cytoplasm.

Article Snippet: Images were taken at various magnifications with a digital charge-coupled device (CCD) camera (Software digital micrograph; Gatan Inc.).

Techniques: Infection

Journal: ACS Omega

Article Title: Phage Display-Derived Peptide for the Specific Binding of SARS-CoV-2

doi: 10.1021/acsomega.1c04873

Figure Lengend Snippet: Peptide 1 can specifically bind to the SARS-CoV-2 virus. (a) Schematic of the experimental procedure for the characterization of peptide binding with the heat-inactivated SARS-CoV-2 virus using the magnetic bead assay and TEM. (b–e) Biotinylated peptide 1 was immobilized on SA-coated magnetic beads to capture the virus. The gold nanoparticle-labeled SARS-CoV-2 antibody was used to detect the virus. The viruses and gold nanoparticles were visualized using TEM. The influenza virus served as a negative control. (b,c) Four individual magnetic beads with the addition of 0.67 and 0.067 mg/mL of the SARS-CoV-2 target to the surface, showing that gold nanoparticles (dashed red circle) have conjugated onto the magnetic beads. (d,e) Four individual magnetic beads with the addition of 0.67 and 0.067 mg/mL of influenza virus. The scale bar is 100 nm.

Article Snippet: The SARS envelope protein antibody used in the TEM assay was purchased from Novus Biologicals, LLC., Centennial, CO. N-Hydroxysuccinimide (NHS)-activated gold nanoparticles with a diameter of 40 nm were purchased from Cytodiagnostics Inc., Canada.

Techniques: Virus, Binding Assay, Magnetic Beads, Labeling, Negative Control

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Binary and ternary ABO 3 -type SLs. (a) TEM image along with (b) HAADF-STEM image, (c) the corresponding wide-angle ED pattern, and (d) SEM images of the [001] SL -oriented b-ABO 3 -type domains assembled from 8.6 nm CsPbBr 3 cubes and 16.5 nm NaGdF 4 spheres. (e, h) AFM height images of spheres- and cubes-terminated b-ABO 3 -type domains, respectively, along with (f, i) the height analysis of the profiles indicated in (e, h), (g, j) AFM three-dimensional images with the respective models. (k) TEM image along with (l) HAADF-STEM image, (m) the corresponding wide-angle ED pattern, and (n) SEM image of the [001] SL -oriented b-ABO 3 -type domains assembled from 8.6 nm CsPbBr 3 cubes and 19.8 nm Fe 3 O 4 spheres. (o) TEM image along with (p) HAADF-STEM image and (q) the corresponding wide-angle ED pattern of the [001]-oriented t-ABO 3 -type SL domains assembled from 8.6 nm CsPbBr 3 cubes, 11.7 nm PbS truncated cuboids, and 21.5 nm Fe 3 O 4 spheres. (r) HAADF-STEM image of a t-ABO 3 -type SL domain in [111] SL orientation assembled from 8.6 nm CsPbBr 3 , 11.7 nm PbS, and 25.1 nm Fe 3 O 4 NCs; upper inset shows the model of [111] SL -oriented t-ABO 3 unit cell, and lower inset shows small-angle ED pattern. Insets in (a, k, o) represent binary and ternary ABO 3 -type lattices according to the preferential NCs orientations, with Fe 3 O 4 shown as gray spheres, NaGdF 4 as yellowish spheres, CsPbBr 3 as blue cubes, and PbS as red truncated cubes. The origin of wide-angle ED reflections in (c, m, q) is color-coded to match the NCs in insets.

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Binary NaCl-type SL. (a) TEM image, (upper right inset) HAADF-STEM image, along with the corresponding (bottom inset) small-angle and (b) wide-angle ED patterns of a SL domain in [001] SL orientation assembled from 8.6 nm CsPbBr 3 cubes and 18.6 nm NaGdF 4 NCs. The upper left inset in (a) represents the NaCl-type unit cell according to the preferential cube’s orientation.

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Binary AlB 2 -type SLs obtained combining 8.6 nm CsPbBr 3 with (a–e) 19.8 nm Fe 3 O 4 and (f–j) 16.5 nm NaGdF 4 NCs. (a, b) TEM and (c) HAADF-STEM images of a single domain in [120] SL orientation, along with the corresponding (d) small-angle and (e) wide-angle ED patterns. (f, g) TEM and (h) HAADF-STEM images of a single domain in [001] SL orientation, along with the corresponding (i) small-angle and (j) wide-angle ED patterns. Insets in (e, j) show the orientations of CsPbBr 3 NCs in the SL domains with respect to the electron beam (normal to the image plane).

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Structural characterization of a binary AlB 2 -type SL comprising 5.3 nm CsPbBr 3 and 12.5 nm Fe 3 O 4 NCs. (a) TEM image of [120] SL -oriented domain; inset is the image at higher magnification. (b) Wide-angle ED pattern of a single SL domain in (a). (c) Two-dimensional GISAX scattering pattern, showing long-range order in AlB 2 -type binary domains. (d) The unit cell of AlB 2 -type SL. (e) Small-angle ED pattern of a domain shown in (a). (f) HAADF-STEM image of the [120] SL -oriented domain. (g) EDX-STEM maps for Fe (gray, K-line) and Pb (blue, L-line) of the [120] SL -oriented domain. (h, k, n) Crystallographic models of [120] SL , [001] SL , and [010] SL -oriented AlB 2 lattice, respectively. (i, j) Low- and high-magnification TEM images of an [001] SL -oriented domain. (l, m) Low- and high-magnification TEM images of a [010] SL -oriented domain; insets in (i, l) are images obtained by template-matching analysis of corresponding TEM images.

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: An AB 2 -type binary SL assembled from CsPbBr 3 nanocubes and Fe 3 O 4 nanospheres. (a) TEM image of a SL assembled by 8.6 nm CsPbBr 3 and 19.8 nm Fe 3 O 4 NCs (γ = 0.414), along with the corresponding (inset) small-angle ED pattern, (b) HAADF-STEM image, and (c) wide-angle ED pattern. (d) Comparison of AlB 2 (taken as orientation “O2”, see Figure ) and AB 2 structures. Red and green lines show the normals to (111) and (110) CsPbBr 3 lattice planes, respectively, and indicate the directions of reflections in wide-angle ED patterns. (e) HAADF-STEM image showing grain boundary between AlB 2 and AB 2 binary SL domains. (f) Modeled crystallographic projections of cubic and spherical NCs in AB 2 structure. (g) EDX-STEM elemental maps of an AB 2 -type binary SL assembled from 5.3 nm CsPbBr 3 and 14.5 nm Fe 3 O 4 NCs for Pb (blue, L-line) and Fe (red, K-line).

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Binary SLs self-assembled from the mixtures of 5.3 nm CsPbBr 3 and 15.2 nm NaGdF 4 NCs. Increasing the relative concentration of CsPbBr 3 NCs changes the experiment outcome from (a–d) NaCl-type to (e–h) AlB 2 -type with (i–l) AB 2 -type and then to (m, n) ABO 3 -type SLs, as illustrated by (o) the scheme. (a, e, i, m) TEM images of [001] SL projections, along with the corresponding (bottom insets) small-angle ED and (b, f, j, n) wide-angle ED patterns; the respective high-magnification HAADF-STEM images are shown as upper insets. (c, d) HAADF images of [001] SL - and [111] SL -oriented domains. (g) TEM image of [120] SL -oriented domain, along with the corresponding (upper inset) HAADF-STEM image, (bottom inset) small-angle ED, and (h) wide-angle ED patterns. (k) Bright-field and (l) HAADF-STEM images of [001] SL -oriented domain.

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques: Concentration Assay

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Characterization of b-ABO 3 -type SL assembled from 8.6 nm CsPbBr 3 and 10.7–11.7 nm PbS. (a) HAADF-STEM image of a single [001] SL -oriented binary ABO 3 domain comprising of 8.6 nm CsPbBr 3 NCs and 11.7 nm PbS NCs. (b) TEM image of a single b-ABO 3 domain in [001] SL orientation assembled from 8.6 nm CsPbBr 3 NCs and 10.7 nm PbS NCs, together with the respective (c) small-angle and (d) wide-angle ED patterns. Diffraction arcs are colored to show their origin from CsPbBr 3 and PbS NCs presented as insets. Inset in (a) shows the binary ABO 3 lattice and illustrates the relative position and orientation of NCs. (e) Crystallographic model of a [001] SL -oriented ABO 3 lattice, along with HAADF-STEM image and respective EDX-STEM maps for S (red, K-line), Pb (blue, L-line), Cs (green, L-line), and Br (yellow, K-line).

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: NaCl-type binary SLs from 8.6 nm CsPbBr 3 NCs combined with truncated cuboid PbS NCs. (a) TEM image of a monolayer domain. (b, c) HAADF-STEM images of SL domains with an increasing number of layers. (e, f) TEM images of [001] SL -oriented SL domains at different magnification, along with the (g) wide-angle and (h) small-angle ED patterns measured from the domain shown in (f); the reflections from CsPbBr 3 and PbS NCs are colored to match the NCs in the structural model (d). Images from (a, c, f–h) were obtained with 10.7 nm PbS NCs (γ = 0.778) and from (b, e) with 11.7 nm PbS NCs (γ = 0.720).

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: CuAu- and AlB 2 -type binary SLs assembled from truncated cuboid 10.7 nm PbS NCs and, respectively, 8.6 and 5.3 nm CsPbBr 3 cubes. (a) TEM image of a single CuAu-type SL domain in [101] SL orientation, along with the corresponding (inset) small-angle ED and (b) wide-angle ED patterns (the origin of the reflections is color-coded to match the NCs in the model shown as inset). (c) CuAu unit cell and crystallographic model of [101] SL -oriented lattice assuming preferable orientations of NCs in agreement with ED. (d) HAADF-STEM images of a SL domain taken at 0° and 45° tilting angles around [010] SL that correspond to [101] SL and [001] SL orientations, respectively; crystallographic model of [001] SL -oriented CuAu-type lattice is depicted in the inset of (e). (f) EDX-STEM elemental maps recorded from a [001] SL -oriented domain shown in (e). (g) TEM image of AlB 2 -type SL with twist grain boundaries between [001] SL - (magnified in upper inset) and [010] SL -oriented (magnified in bottom inset) domains. (h) HAADF-STEM, high-magnification TEM image (upper inset), and crystallographic model (bottom inset) along with (i) wide-angle ED pattern of [120] SL -oriented AlB 2 -type SL. Bottom and upper ([120] SL orientation) insets in (i) represent the unit cell of AlB 2 -type SL with orientations of NCs that result in the most intense wide-angle ED spots marked in red (PbS) and blue (CsPbBr 3 ).

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Binary SLs obtained from FAPbBr 3 nanocubes. (a) TEM and HAADF-STEM (top right panel) images of a b-ABO 3 -type SL assembled from 9 nm FAPbBr 3 and 19.5 nm NaGdF 4 NCs; SL model is shown in the bottom right panel. (b, c) Bright-field STEM images of, respectively, an [120]-oriented AlB 2 -type and [001] SL -oriented AB 2 -type SL domains comprising 9 nm FAPbBr 3 and 15.1 nm NaGdF 4 NCs. (d) HAADF-STEM image of an [111]-oriented NaCl-type SL domain comprising 5.7 nm FAPbBr 3 and 15.1 nm NaGdF 4 NCs. (e) Bright-field STEM image of a columnar AB-type SL domain obtained from 5.7 nm FAPbBr 3 NCs and 12.5 nm LaF 3 nanodisks. (f) TEM and (g) HAADF-STEM images of lamellar SL obtained from 5.7 nm FAPbBr 3 NCs and 12.5 nm LaF 3 nanodisks; EDX-STEM elemental maps for La (magenta, L-line) and Pb (blue, L-line) are shown in the inset in (g). Insets in (b–d) are SL models.

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques:

Journal: ACS Nano

Article Title: Structural Diversity in Multicomponent Nanocrystal Superlattices Comprising Lead Halide Perovskite Nanocubes

doi: 10.1021/acsnano.1c10702

Figure Lengend Snippet: Self-assembly of perovskite NCs at the liquid–air interface. (a) Illustration of the assembly process: NCs dispersed in nonpolar solvents are cast onto the surface of glyceryl triacetate in a Teflon well or Petri dish, which is then covered with glass or larger Petri dish, respectively; ordered SL film floating on the subphase is formed upon evaporation the solvent. (b–d) TEM images of 9 nm CsPbBr 3 NC monolayer obtained from octane on glyceryl triacetate. (e–g) TEM images of AB-type monolayer (obtained from dodecane) and NaCl- and AlB 2 -type films (obtained from decane), respectively, comprising 8.6 nm CsPbBr 3 and 19.8 nm Fe 3 O 4 NCs.

Article Snippet: Self-assembly of NCs was carried out using a drying-mediated method on carbon-coated TEM grids (carbon type B, Ted Pella, Formvar protective layer was removed by immersing the grid in toluene for 10 s), HF-treated silicon, and silicon nitride TEM windows (Agar Scientific, Norcada).

Techniques: Evaporation