Journal: Bone Reports

Article Title: Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells

doi: 10.1016/j.bonr.2024.101816

Figure Lengend Snippet: Three-dimensional osteogenic differentiation culture using human induced pluripotent stem cells (hiPSCs). ( a ) Schema showing three-dimensional osteogenic differentiation culture using hiPSCs. ( b ) Sections of the sponge were incubated for 28 days, and then stained with Alizarin Red S. Positive areas can be observed at the edges of the sponge. COL1A1 expression was analyzed using confocal laser microscopy. ( c ) Atelocollagen sponge without seeded hiPSCs. ( d)–(g ) Section of the sponge cultured for 28 days after seeding hiPSCs. Blue fluorescence indicates the nuclei and green fluorescence indicates COL1A1 expression. ( d ), ( e ) Cells stained only with DAPI without staining for COL1A1. ( e ) An enlarged image of a part of ( d ). Both ( d ) and ( e ) show the presence of nuclei at the edges and inside the sponge. ( f ), ( g ) Cells with additional staining for COL1A1. ( g ) An enlarged image of a region from ( f ). COL1A1-positive areas can be observed at the edges and inside the sponge. COL1A1, type I collagen alpha 1; hiPSC, human induced pluripotent stem cell.

Article Snippet: These sections were incubated with a primary antibody against type I collagen alpha 1 (COL1A1; diluted 1:100; 322-COLTt, PhosphoSolutions, Denver, CO, USA) at 37 °C in a humid chamber for 1 h. Subsequently, the sections were incubated at 25 °C for 1 h with the secondary antibody Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488; diluted 1:1000; Thermo Fisher Scientific).

Techniques: Incubation, Staining, Expressing, Microscopy, Cell Culture, Fluorescence

Journal: Bone Reports

Article Title: Identifying the best reference gene for RT-qPCR analyses of the three-dimensional osteogenic differentiation of human induced pluripotent stem cells

doi: 10.1016/j.bonr.2024.101816

Figure Lengend Snippet: Primers used for amplifying the target genes in the present study.

Article Snippet: These sections were incubated with a primary antibody against type I collagen alpha 1 (COL1A1; diluted 1:100; 322-COLTt, PhosphoSolutions, Denver, CO, USA) at 37 °C in a humid chamber for 1 h. Subsequently, the sections were incubated at 25 °C for 1 h with the secondary antibody Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488; diluted 1:1000; Thermo Fisher Scientific).

Techniques: Functional Assay, SYBR Green Assay, Marker, Gene Expression

Journal: BMC Oral Health

Article Title: Effect of polyhydroxy-terminated PAMAM dendrimer on dentin matrix metalloproteinases within the hybrid layers

doi: 10.1186/s12903-023-02841-2

Figure Lengend Snippet: The concentration of ICTP released from demineralized dentin in control group and PAMAM-OH group after 2 weeks of incubation in deionized water (DW) and artificial saliva (AS). Data are expressed as mean ± standard deviation. For the comparison of the four subgroups, columns identified with the different lowercase letters are significantly different ( p < 0.05)

Article Snippet: The measurement was performed with a spectrometer (Beckman Coulter, Inc., Indianapolis, IN, USA) at the absorbance of 450 nm and the amount of ICTP was calculated according to the standard curve constructed using the standards of known concentrations provided in the ICTP ELISA kit (Catalogue no. CSB-E11224 h, Cusabio, Wuhan, China).

Techniques: Concentration Assay, Control, Incubation, Standard Deviation, Comparison