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Image Search Results
Journal: bioRxiv
Article Title: Brainstem development requires galactosylceramidase and is critical for the pathogenesis of Krabbe Disease
doi: 10.1101/2020.03.25.007542
Figure Lengend Snippet: Tamoxifen was injected into Thy1-Cre/ER T2 ; tdTomato starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, Olig2, GFAP and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
Article Snippet: Primary antibodies used were GAPDH (Chemicon), β-tubulin (Novus Biologicals),
Techniques: Injection