tdtomato Search Results


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Tamoxifen was injected into Thy1-Cre/ER T2 ; <t>tdTomato</t> starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, <t>Olig2,</t> <t>GFAP</t> and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
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Tamoxifen was injected into Thy1-Cre/ER T2 ; <t>tdTomato</t> starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, <t>Olig2,</t> <t>GFAP</t> and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
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Tamoxifen was injected into Thy1-Cre/ER T2 ; <t>tdTomato</t> starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, <t>Olig2,</t> <t>GFAP</t> and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
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Tamoxifen was injected into Thy1-Cre/ER T2 ; <t>tdTomato</t> starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, <t>Olig2,</t> <t>GFAP</t> and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
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Tamoxifen was injected into Thy1-Cre/ER T2 ; <t>tdTomato</t> starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, <t>Olig2,</t> <t>GFAP</t> and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
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Tamoxifen was injected into Thy1-Cre/ER T2 ; <t>tdTomato</t> starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, <t>Olig2,</t> <t>GFAP</t> and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
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Tamoxifen was injected into Thy1-Cre/ER T2 ; <t>tdTomato</t> starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, <t>Olig2,</t> <t>GFAP</t> and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.
Paav Cag Tdtomato, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Tamoxifen was injected into Thy1-Cre/ER T2 ; tdTomato starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, Olig2, GFAP and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.

Journal: bioRxiv

Article Title: Brainstem development requires galactosylceramidase and is critical for the pathogenesis of Krabbe Disease

doi: 10.1101/2020.03.25.007542

Figure Lengend Snippet: Tamoxifen was injected into Thy1-Cre/ER T2 ; tdTomato starting at P2, and the mice were dissected at P10 or P30 ( a ). Analysis of major sub-brain regions such as cerebellum, brainstem and cerebral cortex ( b ) showed that more than 65% of Thy1-positive cells overlap with the tdTomato in the hindbrain. The co-localization rate was about 50% in the cerebral cortex ( c ). The tdTomato signals were not overlapped with other cell markers, Olig2, GFAP and Iba1, indicating neuron-specific recombination ( D ). Scale bar=50 µ m.

Article Snippet: Primary antibodies used were GAPDH (Chemicon), β-tubulin (Novus Biologicals), tdTomato (Origene), TLR2 (R&D systems), GFAP and HA (all from Sigma-Aldrich).

Techniques: Injection