tc20 Search Results


cell counter  (Bio-Rad)
97
Bio-Rad cell counter
Cell Counter, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/TC20+Automated+Cell+Counter/pmc11101354-328-17-20
Average 97 stars, based on 1 article reviews
cell counter - by Bioz Stars, 2026-09
97/100 stars
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slide  (Bio-Rad)
96
Bio-Rad slide
Slide, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/Cell+Counting+Slides+for+TC10+%2FTC20+Cell+Counter/pmc06474884-219-14-28
Average 96 stars, based on 1 article reviews
slide - by Bioz Stars, 2026-09
96/100 stars
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tc20 bio rad automated cell counter  (Bio-Rad)
99
Bio-Rad tc20 bio rad automated cell counter
Tc20 Bio Rad Automated Cell Counter, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/TC20+Automated+Cell+Counter+Firmware/pm41520091-422-11-12
Average 99 stars, based on 1 article reviews
tc20 bio rad automated cell counter - by Bioz Stars, 2026-09
99/100 stars
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tc20 data analyzer  (Bio-Rad)
99
Bio-Rad tc20 data analyzer
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Tc20 Data Analyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/TC20+Data+Analyzer+Software/pmc11402648-115-0-4
Average 99 stars, based on 1 article reviews
tc20 data analyzer - by Bioz Stars, 2026-09
99/100 stars
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tc20 automated cell counter  (Bio-Rad)
93
Bio-Rad tc20 automated cell counter
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Tc20 Automated Cell Counter, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/TC20+Automated+Cell+Counter+with+Thermal+Label+Printer/bio_rxiv__555755-78-25-29
Average 93 stars, based on 1 article reviews
tc20 automated cell counter - by Bioz Stars, 2026-09
93/100 stars
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temperature controller tc 20/tek 350  (Anton Paar)
90
Anton Paar temperature controller tc 20/tek 350
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Temperature Controller Tc 20/Tek 350, supplied by Anton Paar, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/temperature+controller+tc+20+tek+350/10__1002_slash_macp__202000132-327-10-15
Average 90 stars, based on 1 article reviews
temperature controller tc 20/tek 350 - by Bioz Stars, 2026-09
90/100 stars
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automated cell counter tc 20  (Becton Dickinson)
90
Becton Dickinson automated cell counter tc 20
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Automated Cell Counter Tc 20, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/automated+cell+counter+tc+20/pm33559879-83-10-14
Average 90 stars, based on 1 article reviews
automated cell counter tc 20 - by Bioz Stars, 2026-09
90/100 stars
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tc20 automated cell counter  (Biora Inc)
90
Biora Inc tc20 automated cell counter
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Tc20 Automated Cell Counter, supplied by Biora Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/tc20+automated+cell+counter/pmc05565430-442-21-25
Average 90 stars, based on 1 article reviews
tc20 automated cell counter - by Bioz Stars, 2026-09
90/100 stars
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condenser microphone behringer tc20  (BEHRINGER International GmbH)
90
BEHRINGER International GmbH condenser microphone behringer tc20
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Condenser Microphone Behringer Tc20, supplied by BEHRINGER International GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/condenser+microphone+behringer+tc20/pmc08960899-264-4-3
Average 90 stars, based on 1 article reviews
condenser microphone behringer tc20 - by Bioz Stars, 2026-09
90/100 stars
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automated cell counter (e.g., bio-rad tc20) or neubauer hemocytometer  (Merck KGaA)
90
Merck KGaA automated cell counter (e.g., bio-rad tc20) or neubauer hemocytometer
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Automated Cell Counter (E.G., Bio Rad Tc20) Or Neubauer Hemocytometer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/automated+cell+counter++e+g+++bio+rad+tc20++or+neubauer+hemocytometer/pm28777439-104-220-222
Average 90 stars, based on 1 article reviews
automated cell counter (e.g., bio-rad tc20) or neubauer hemocytometer - by Bioz Stars, 2026-09
90/100 stars
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three-way tubing connector tc-20/3  (Small Parts Inc)
90
Small Parts Inc three-way tubing connector tc-20/3
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Three Way Tubing Connector Tc 20/3, supplied by Small Parts Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/three+way+tubing+connector+tc+20+3/pm12209897-52-19-23
Average 90 stars, based on 1 article reviews
three-way tubing connector tc-20/3 - by Bioz Stars, 2026-09
90/100 stars
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tetracycline tc 20  (Applichem inc)
90
Applichem inc tetracycline tc 20
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Tetracycline Tc 20, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tc20/tetracycline+tc+20/pmc06216589-49-37-5
Average 90 stars, based on 1 article reviews
tetracycline tc 20 - by Bioz Stars, 2026-09
90/100 stars
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PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad TC20 Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).

Journal: iScience

Article Title: A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation

doi: 10.1016/j.isci.2024.110762

Figure Lengend Snippet: PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad TC20 Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).

Article Snippet: TC20 data analyzer , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Western Blot, Control, Labeling, Flow Cytometry, BrdU Incorporation Assay, Expressing, Activity Assay, Marker, Sublimation, Phospho-proteomics, Two Tailed Test

Journal: iScience

Article Title: A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation

doi: 10.1016/j.isci.2024.110762

Figure Lengend Snippet:

Article Snippet: TC20 data analyzer , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Transduction, Virus, Recombinant, Formulation, Random Hexamer, Reverse Transcription, Western Blot, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Expressing, Mutagenesis, Plasmid Preparation, Software