Journal: iScience

Article Title: Single-cell RNA sequencing identifies a migratory keratinocyte subpopulation expressing THBS1 in epidermal wound healing

doi: 10.1016/j.isci.2022.104130

Figure Lengend Snippet:

Article Snippet: TCLPA54 , Tocris , Cat# 4708/10.

Techniques: Recombinant, Transfection, TUNEL Assay, Imaging, Software

Journal: Structure (London, England : 1993)

Article Title: Bilayer lipids modulate ligand binding to atypical chemokine receptor 3.

doi: 10.1016/j.str.2024.04.018

Figure Lengend Snippet: Figure 2. Favorable effects of anionic lipid bilayers on ligand binding to ACKR3 are ligand specific pKi (log Ki) values of CXCL12 (A), small-molecule agonist VUF11207 (B), and cyclic peptide agonist TC14012 (C) determined in competition binding experiments using the SPA technique and ACKR3 POPC (PC), 3/2 POPC/POPG (PC/PG), 3/2 POPC/POPS (PC/PS), and 9/1 POPC/Cardiolipin (PC/CL) NDs. (A) The affinity for CXCL12 is increased in anionic NDs as shown in the kinetics experiments (Figure 1F). (B and C) The pKi values of VUF11207 and TC14012 to ACKR3 were either unchanged or decreased upon introduction of a negative bilayer charge. (A–C) Data points correspond to three independent experiments performed in duplicates. Mean ± SEM are shown. One-way ANOVA with Dunnett’s multiple comparisons test was applied. Exact values and representative curves are provided in Table S1 and Figure S6, respectively. Statistical comparisons between anionic NDs are given in Table S2. POPC data of CXCL12 and TC14012 are from Eberle and Gustavsson.39

Article Snippet: For competition binding experiments, samples were prepared as for the association experiments but supplemented with various concentrations of unlabeled CXCL11 [Peprotech;6], CXCL12, VUF11207 [Sigma-Aldrich;65] or TC14012 [Tocris;66] prior to the immobilization.

Techniques: Ligand Binding Assay, Binding Assay