tbid Search Results


aavs1 isam  (Addgene inc)
92
Addgene inc aavs1 isam
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Aavs1 Isam, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/10__7554_slash_elife__94884-177-1-6?v=Addgene+inc
Average 92 stars, based on 1 article reviews
aavs1 isam - by Bioz Stars, 2026-08
92/100 stars
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human recombinant tbid  (Enzo Biochem)
90
Enzo Biochem human recombinant tbid
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Human Recombinant Tbid, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/10__1074_slash_jbc__m705671200-103-65-69?v=Enzo+Biochem
Average 90 stars, based on 1 article reviews
human recombinant tbid - by Bioz Stars, 2026-08
90/100 stars
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mgit tbid kit  (Becton Dickinson)
90
Becton Dickinson mgit tbid kit
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Mgit Tbid Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pm35813561-50-7-10?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
mgit tbid kit - by Bioz Stars, 2026-08
90/100 stars
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tbid  (AnaSpec)
90
AnaSpec tbid
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Tbid, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pmc03642864-96-18-22?v=AnaSpec
Average 90 stars, based on 1 article reviews
tbid - by Bioz Stars, 2026-08
90/100 stars
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ul8-v5-tbid (24) inserts  (GenScript corporation)
90
GenScript corporation ul8-v5-tbid (24) inserts
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Ul8 V5 Tbid (24) Inserts, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pm37772824-210-32-43?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
ul8-v5-tbid (24) inserts - by Bioz Stars, 2026-08
90/100 stars
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anti-bid/-tbid  (Becton Dickinson)
90
Becton Dickinson anti-bid/-tbid
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Anti Bid/ Tbid, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pmc07301057__41423_2020_485_MOESM1_ESM-19-5-6?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
anti-bid/-tbid - by Bioz Stars, 2026-08
90/100 stars
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recombinant tbid (rtbid)  (Mitochon Pharmaceuticals)
90
Mitochon Pharmaceuticals recombinant tbid (rtbid)
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Recombinant Tbid (Rtbid), supplied by Mitochon Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pm27320914-143-1-0?v=Mitochon+Pharmaceuticals
Average 90 stars, based on 1 article reviews
recombinant tbid (rtbid) - by Bioz Stars, 2026-08
90/100 stars
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bid cleaved (ab-1, tbid) antibody  (Merck KGaA)
90
Merck KGaA bid cleaved (ab-1, tbid) antibody
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Bid Cleaved (Ab 1, Tbid) Antibody, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pmc04258573-83-57-58?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
bid cleaved (ab-1, tbid) antibody - by Bioz Stars, 2026-08
90/100 stars
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tbid peptide  (AnaSpec)
90
AnaSpec tbid peptide
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Tbid Peptide, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pm25028717-179-37-41?v=AnaSpec
Average 90 stars, based on 1 article reviews
tbid peptide - by Bioz Stars, 2026-08
90/100 stars
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recombinant tbid  (AnaSpec)
90
AnaSpec recombinant tbid
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Recombinant Tbid, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/10__1042_slash_bj20130147-82-18-22?v=AnaSpec
Average 90 stars, based on 1 article reviews
recombinant tbid - by Bioz Stars, 2026-08
90/100 stars
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rabbit polyclonal anti-tbid antibodies  (Becton Dickinson)
90
Becton Dickinson rabbit polyclonal anti-tbid antibodies
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Rabbit Polyclonal Anti Tbid Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pm18485277-101-9-14?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
rabbit polyclonal anti-tbid antibodies - by Bioz Stars, 2026-08
90/100 stars
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immunofluorescent staining for tbid  (Verlag GmbH)
90
Verlag GmbH immunofluorescent staining for tbid
Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by <t>AAVS1</t> specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).
Immunofluorescent Staining For Tbid, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tbid/pm24142587-133-18-3?v=Verlag+GmbH
Average 90 stars, based on 1 article reviews
immunofluorescent staining for tbid - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by AAVS1 specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).

Journal: eLife

Article Title: A novel human pluripotent stem cell-based gene activation system identifies IGFBP2 as a mediator in the production of hematopoietic progenitors in vitro

doi: 10.7554/elife.94884

Figure Lengend Snippet: Figure 2. The inducible SAM (iSAM) cassette successfully mediates activation of endogenous gene expression upon doxycycline (DOX) induction. (A) Schematic of the iSAM cassette containing the TET-on system under the control of EF1α and dCAS9-P2A-MS2-p65-HSF1-T2A-mCherry under the rTTA responsive elements, separated by genetic silencer and flanked by AAVS1 specific homology arms. (B) RUNX1C gene expression activation after transient transfection of the iSAM plasmid and gRNAs in presence or absence of DOX in human induced pluripotent stem cell (iPSC) line (n=3 from independent transfections). (C) RUNX1 protein expression upon iSAM activation after transient transfection of the iSAM plasmid and gRNAs with DOX in human iPSC line detected by immunostaining. (D) Expression of the iSAM cassette reported by mCherry tag during the differentiation protocol, the representative images (bright field - BF, and fluorescence) show embryoid bodies at day 3 of differentiation. (E) Schematic of the gRNA 2.1 containing the capture sequence for detection during the single-cell RNA sequencing (scRNAseq) pipeline. (F) RUNX1C gene activation level obtained using either the gRNA 2.0 or 2.1 backbone (n=3 from independent transfections of the 4 different gRNAs). (G) Statistical analysis of the gRNAs activation level showing no significant variation following addition of the capture sequence (n=3 for each of the 4 different gRNAs).

Article Snippet: The AAVS1- iSAM are available on Addgene (RRID:Addgene_211495 ) and the gRNA 2.1 plasmids (RRID:Addgene_211496).

Techniques: Activation Assay, Gene Expression, Control, Transfection, Plasmid Preparation, Expressing, Immunostaining, Fluorescence, Sequencing, RNA Sequencing