tat Search Results


santa cruz biotechnology sc 376292  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology santa cruz biotechnology sc 376292
Santa Cruz Biotechnology Sc 376292, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/santa cruz biotechnology sc 376292/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
santa cruz biotechnology sc 376292 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
tat gap19  (Tocris)
94
Tocris tat gap19
Tat Gap19, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tat gap19/product/Tocris
Average 94 stars, based on 1 article reviews
tat gap19 - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
thrombin  (R&D Systems)
90
R&D Systems thrombin
Thrombin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thrombin/product/R&D Systems
Average 90 stars, based on 1 article reviews
thrombin - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
tat  (Novus Biologicals)
94
Novus Biologicals tat
Tat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tat/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
tat - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
tat beclin 1 d11 peptide  (Novus Biologicals)
94
Novus Biologicals tat beclin 1 d11 peptide
Tat Beclin 1 D11 Peptide, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tat beclin 1 d11 peptide/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
tat beclin 1 d11 peptide - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
18b recombinant human sox2 novus biologicals nbp2  (Novus Biologicals)
92
Novus Biologicals 18b recombinant human sox2 novus biologicals nbp2
Figure 3. The <t>SOX2</t> C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.
18b Recombinant Human Sox2 Novus Biologicals Nbp2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/18b recombinant human sox2 novus biologicals nbp2/product/Novus Biologicals
Average 92 stars, based on 1 article reviews
18b recombinant human sox2 novus biologicals nbp2 - by Bioz Stars, 2026-04
92/100 stars
  Buy from Supplier
tat cre delivery  (Addgene inc)
93
Addgene inc tat cre delivery
Figure 3. The <t>SOX2</t> C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.
Tat Cre Delivery, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tat cre delivery/product/Addgene inc
Average 93 stars, based on 1 article reviews
tat cre delivery - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
tat beclin1  (Selleck Chemicals)
93
Selleck Chemicals tat beclin1
Figure 3. The <t>SOX2</t> C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.
Tat Beclin1, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tat beclin1/product/Selleck Chemicals
Average 93 stars, based on 1 article reviews
tat beclin1 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
recombinant hiv 1 tat  (Biosynth Carbosynth)
86
Biosynth Carbosynth recombinant hiv 1 tat
Figure 3. The <t>SOX2</t> C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.
Recombinant Hiv 1 Tat, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant hiv 1 tat/product/Biosynth Carbosynth
Average 86 stars, based on 1 article reviews
recombinant hiv 1 tat - by Bioz Stars, 2026-04
86/100 stars
  Buy from Supplier
hiv 1 tat antibody  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology hiv 1 tat antibody
Figure 3. The <t>SOX2</t> C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.
Hiv 1 Tat Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiv 1 tat antibody/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
hiv 1 tat antibody - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
biotin switch assay  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology biotin switch assay
Figure 3. The <t>SOX2</t> C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.
Biotin Switch Assay, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotin switch assay/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
biotin switch assay - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
peptide  (Novus Biologicals)
93
Novus Biologicals peptide
Figure 3. The <t>SOX2</t> C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.
Peptide, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/peptide/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
peptide - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

Image Search Results


Figure 3. The SOX2 C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.

Journal: Cell reports

Article Title: Nuclear and cytosolic fractions of SOX2 synergize as transcriptional and translational co-regulators of cell fate.

doi: 10.1016/j.celrep.2024.114807

Figure Lengend Snippet: Figure 3. The SOX2 C-terminal domain modulates protein synthesis in the cytosol (A) Schematic of SOX2 domain separation with indication of DNA-binding high-mobility group (HMG), RNA binding motif (RBM), and transactivation domain (TAD). The indicated demarcation line (red) was chosen to subdivide the human SOX2 protein (residues 1–317, full length) into stably expressing N-terminal (residues 1–179) and C-terminal (residues 180–317) fragments. For extended comparison, a nuclear export sequence (NES) was selectively fused to the C-terminal fragment. (B) Subcellular localization of the indicated SOX2 expression constructs as investigated by life microscopy: mCherry-fusion protein (red), nuclei (Hoechst, blue), and transmitted light (VIS). Scale bar: 10 mm.

Article Snippet: Chemicals, peptides, and recombinant proteins Cycloheximide Sigma C7698 Puromycin Applichem A2856 Geneticin Gibco #11-811-031 O-propargyl-puromycin (OPP) Thermo-Fisher Click-iT Plus OPP, C10456 Oligomycin Sigma #75351 2-Deoxy-D-glucose Sigma D3179 2-Nitrobenzyl-desoxyglucose (2-NBDG) Thermo-Fisher N13195 Carbonyl-cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) Sigma C2920 Rotenone Sigma R8875 Biotin Sigma B4639 Heparin Ratiopharm #06884371 Insulin Lonza CC-4021G Hydrocortisone Lonza CC-4031G Epidermal growth factor (EGF) Sigma E9644 Fibroblast growth factor (FGF) PeproTech #100-18B Recombinant human SOX2 Novus Biologicals NBP2-35268 Critical commercial assays FluoroTect GreenLys in vitro Translation Labeling System Promega #L5001 Click-iTTM Plus OPP Alexa FluorTM 488 Protein Synthesis Assay Kit Thermo-Fisher C10456 SMARTer smRNA-Seq Kit for Illumina Clontech, Takara Bio #635031 TruSeq Stranded Total RNA Kit V2.0 Illumina #20020594 Deposited data RNAseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13098 RIBOseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13099 RNAseq and RIBOseq analysis scripts This paper Zenodo (https://zenodo.org/) https://doi.org/10.5281/zenodo.13752731 MS hit lists This paper PRIDE (https://www.ebi.ac.uk./pride/, Dataset identifier: PXD015157 and https://doi.org/10.6019/PXD015157. (Continued on next page) 22 Cell Reports 43, 114807, October 22, 2024

Techniques: Binding Assay, RNA Binding Assay, Stable Transfection, Expressing, Comparison, Sequencing, Construct, Microscopy

Figure 5. Functional in vitro validation of SOX2 C terminus-imposed translational adaptations (A) Relative changes in glucose uptake in response to SOX2 (fragment) induction in T47D cells. Indicated are MFI shifts at l = 488 nm (D mean 488 nm) reflecting SOX2 (fragment)-induced changes to the incorporation rate of glucose uptake reporter 2-NBDG. The endogenous uptake rate into DOX-exposed but empty control cells is set to baseline. Dots indicate mean intensity shift in 4 independent experiments. (B) Representative example of a metabolic Seahorse analysis indicating aggravated sugar consumption (followed as extracellular acidification rate [ECAR]) in starved T47D cells expressing either full-length SOX2 (1–317, red) or an NES/C-terminal segment thereof (180–317, green) vs. equally treated empty control cells (black) or T47D cells expressing the SOX2 N terminus (1–179, blue). Dots reflect means of 4 or more technical replicates.

Journal: Cell reports

Article Title: Nuclear and cytosolic fractions of SOX2 synergize as transcriptional and translational co-regulators of cell fate.

doi: 10.1016/j.celrep.2024.114807

Figure Lengend Snippet: Figure 5. Functional in vitro validation of SOX2 C terminus-imposed translational adaptations (A) Relative changes in glucose uptake in response to SOX2 (fragment) induction in T47D cells. Indicated are MFI shifts at l = 488 nm (D mean 488 nm) reflecting SOX2 (fragment)-induced changes to the incorporation rate of glucose uptake reporter 2-NBDG. The endogenous uptake rate into DOX-exposed but empty control cells is set to baseline. Dots indicate mean intensity shift in 4 independent experiments. (B) Representative example of a metabolic Seahorse analysis indicating aggravated sugar consumption (followed as extracellular acidification rate [ECAR]) in starved T47D cells expressing either full-length SOX2 (1–317, red) or an NES/C-terminal segment thereof (180–317, green) vs. equally treated empty control cells (black) or T47D cells expressing the SOX2 N terminus (1–179, blue). Dots reflect means of 4 or more technical replicates.

Article Snippet: Chemicals, peptides, and recombinant proteins Cycloheximide Sigma C7698 Puromycin Applichem A2856 Geneticin Gibco #11-811-031 O-propargyl-puromycin (OPP) Thermo-Fisher Click-iT Plus OPP, C10456 Oligomycin Sigma #75351 2-Deoxy-D-glucose Sigma D3179 2-Nitrobenzyl-desoxyglucose (2-NBDG) Thermo-Fisher N13195 Carbonyl-cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) Sigma C2920 Rotenone Sigma R8875 Biotin Sigma B4639 Heparin Ratiopharm #06884371 Insulin Lonza CC-4021G Hydrocortisone Lonza CC-4031G Epidermal growth factor (EGF) Sigma E9644 Fibroblast growth factor (FGF) PeproTech #100-18B Recombinant human SOX2 Novus Biologicals NBP2-35268 Critical commercial assays FluoroTect GreenLys in vitro Translation Labeling System Promega #L5001 Click-iTTM Plus OPP Alexa FluorTM 488 Protein Synthesis Assay Kit Thermo-Fisher C10456 SMARTer smRNA-Seq Kit for Illumina Clontech, Takara Bio #635031 TruSeq Stranded Total RNA Kit V2.0 Illumina #20020594 Deposited data RNAseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13098 RIBOseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13099 RNAseq and RIBOseq analysis scripts This paper Zenodo (https://zenodo.org/) https://doi.org/10.5281/zenodo.13752731 MS hit lists This paper PRIDE (https://www.ebi.ac.uk./pride/, Dataset identifier: PXD015157 and https://doi.org/10.6019/PXD015157. (Continued on next page) 22 Cell Reports 43, 114807, October 22, 2024

Techniques: Functional Assay, In Vitro, Biomarker Discovery, Control, Expressing

Figure 6. A disease-linked human mutant abolishes the translational role of SOX2 (A) Western blot analyses verify inducibility of the indicated SOX2 variants expressed as mCherry-fusions in T47D. The nucleocytoplasmic transport mutants T116A and T118A, DNA non-binding M49G, and disease-associated SOX2 variants L97P, G130A, and A191T were investigated. Anti-mCherry staining (top) confirms robust induction relative to wild-type transduced cells (blue). Anti-pRPS6(235/236) staining (center) indicates SOX2-imposed changes for all constructs but SOX2(L97P) (red). Anti-RPS6 staining (control, bottom).

Journal: Cell reports

Article Title: Nuclear and cytosolic fractions of SOX2 synergize as transcriptional and translational co-regulators of cell fate.

doi: 10.1016/j.celrep.2024.114807

Figure Lengend Snippet: Figure 6. A disease-linked human mutant abolishes the translational role of SOX2 (A) Western blot analyses verify inducibility of the indicated SOX2 variants expressed as mCherry-fusions in T47D. The nucleocytoplasmic transport mutants T116A and T118A, DNA non-binding M49G, and disease-associated SOX2 variants L97P, G130A, and A191T were investigated. Anti-mCherry staining (top) confirms robust induction relative to wild-type transduced cells (blue). Anti-pRPS6(235/236) staining (center) indicates SOX2-imposed changes for all constructs but SOX2(L97P) (red). Anti-RPS6 staining (control, bottom).

Article Snippet: Chemicals, peptides, and recombinant proteins Cycloheximide Sigma C7698 Puromycin Applichem A2856 Geneticin Gibco #11-811-031 O-propargyl-puromycin (OPP) Thermo-Fisher Click-iT Plus OPP, C10456 Oligomycin Sigma #75351 2-Deoxy-D-glucose Sigma D3179 2-Nitrobenzyl-desoxyglucose (2-NBDG) Thermo-Fisher N13195 Carbonyl-cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) Sigma C2920 Rotenone Sigma R8875 Biotin Sigma B4639 Heparin Ratiopharm #06884371 Insulin Lonza CC-4021G Hydrocortisone Lonza CC-4031G Epidermal growth factor (EGF) Sigma E9644 Fibroblast growth factor (FGF) PeproTech #100-18B Recombinant human SOX2 Novus Biologicals NBP2-35268 Critical commercial assays FluoroTect GreenLys in vitro Translation Labeling System Promega #L5001 Click-iTTM Plus OPP Alexa FluorTM 488 Protein Synthesis Assay Kit Thermo-Fisher C10456 SMARTer smRNA-Seq Kit for Illumina Clontech, Takara Bio #635031 TruSeq Stranded Total RNA Kit V2.0 Illumina #20020594 Deposited data RNAseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13098 RIBOseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13099 RNAseq and RIBOseq analysis scripts This paper Zenodo (https://zenodo.org/) https://doi.org/10.5281/zenodo.13752731 MS hit lists This paper PRIDE (https://www.ebi.ac.uk./pride/, Dataset identifier: PXD015157 and https://doi.org/10.6019/PXD015157. (Continued on next page) 22 Cell Reports 43, 114807, October 22, 2024

Techniques: Mutagenesis, Western Blot, Binding Assay, Staining, Construct, Control

Figure 7. An advanced model of SOX2 in cell fate control (A) SOX2 establishes contact with DNA recognition motifs via the HMG (blue sphere),14 activates these elements by its pioneering TF activity,72 and thus enforces the transcription of cognate mRNAs. (B) To facilitate nuclear export, physical contact with nucleoporin Nup153 may be established and distinct target genes positioned to the nuclear pore.24

Journal: Cell reports

Article Title: Nuclear and cytosolic fractions of SOX2 synergize as transcriptional and translational co-regulators of cell fate.

doi: 10.1016/j.celrep.2024.114807

Figure Lengend Snippet: Figure 7. An advanced model of SOX2 in cell fate control (A) SOX2 establishes contact with DNA recognition motifs via the HMG (blue sphere),14 activates these elements by its pioneering TF activity,72 and thus enforces the transcription of cognate mRNAs. (B) To facilitate nuclear export, physical contact with nucleoporin Nup153 may be established and distinct target genes positioned to the nuclear pore.24

Article Snippet: Chemicals, peptides, and recombinant proteins Cycloheximide Sigma C7698 Puromycin Applichem A2856 Geneticin Gibco #11-811-031 O-propargyl-puromycin (OPP) Thermo-Fisher Click-iT Plus OPP, C10456 Oligomycin Sigma #75351 2-Deoxy-D-glucose Sigma D3179 2-Nitrobenzyl-desoxyglucose (2-NBDG) Thermo-Fisher N13195 Carbonyl-cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) Sigma C2920 Rotenone Sigma R8875 Biotin Sigma B4639 Heparin Ratiopharm #06884371 Insulin Lonza CC-4021G Hydrocortisone Lonza CC-4031G Epidermal growth factor (EGF) Sigma E9644 Fibroblast growth factor (FGF) PeproTech #100-18B Recombinant human SOX2 Novus Biologicals NBP2-35268 Critical commercial assays FluoroTect GreenLys in vitro Translation Labeling System Promega #L5001 Click-iTTM Plus OPP Alexa FluorTM 488 Protein Synthesis Assay Kit Thermo-Fisher C10456 SMARTer smRNA-Seq Kit for Illumina Clontech, Takara Bio #635031 TruSeq Stranded Total RNA Kit V2.0 Illumina #20020594 Deposited data RNAseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13098 RIBOseq raw sequencing data This paper ArrayExpress (https://www.ebi.ac.uk/ biostudies/arrayexpress) Record number: E-MTAB-13099 RNAseq and RIBOseq analysis scripts This paper Zenodo (https://zenodo.org/) https://doi.org/10.5281/zenodo.13752731 MS hit lists This paper PRIDE (https://www.ebi.ac.uk./pride/, Dataset identifier: PXD015157 and https://doi.org/10.6019/PXD015157. (Continued on next page) 22 Cell Reports 43, 114807, October 22, 2024

Techniques: Control, Activity Assay