Journal: BioMed Research International

Article Title: SNP rs8099917 in Gene IL28B Might Be Associated with Risk of Chronic Infection by HCV but Not with Response to Treatment

doi: 10.1155/2014/748606

Figure Lengend Snippet: Genotypic and allelic frequencies of SNP rs12979860 and rs8099917 in gene IL28B in individuals with chronic hepatitis C virus infection and the control group, Belém, 2012.

Article Snippet: The rs12979860 target sequence was sent to the Applied Biosystems company, which designed the following primer and probe sequences: 5′-GCC TGT CGT GTA CTG AAC CA-3′ (forward), 5′-GCG CGG AGT TGC AAT TCA AC-3′ (reverse), 5′VIC-TGG TTC GCG CTT C-3′ (allele C probe), and 5′FAM-CTG GTT CAC GCC TTC-3′ (allele T probe).

Techniques: Virus, Infection, Control

Journal: BioMed Research International

Article Title: SNP rs8099917 in Gene IL28B Might Be Associated with Risk of Chronic Infection by HCV but Not with Response to Treatment

doi: 10.1155/2014/748606

Figure Lengend Snippet: Association of SNPs rs12979860 and rs8099917 with sustained virologic response in individuals with chronic hepatitis C treated with PEG-IFN and RBV.

Article Snippet: The rs12979860 target sequence was sent to the Applied Biosystems company, which designed the following primer and probe sequences: 5′-GCC TGT CGT GTA CTG AAC CA-3′ (forward), 5′-GCG CGG AGT TGC AAT TCA AC-3′ (reverse), 5′VIC-TGG TTC GCG CTT C-3′ (allele C probe), and 5′FAM-CTG GTT CAC GCC TTC-3′ (allele T probe).

Techniques:

Journal: Cancer Cell International

Article Title: Differential drug resistance acquisition to doxorubicin and paclitaxel in breast cancer cells

doi: 10.1186/s12935-014-0142-4

Figure Lengend Snippet: Impact of PXR activation in DOX and PTX treated cells. (A) PXR mRNA expression with the treatment of DOX and PTX at 0, 4, 8, 12, 24, 36, 48 h. (B) Western blot analysis of PXR with 24 h treatment of DOX and PTX. **, p < 0.01 (n = 3). (C) RT-PCR analysis of PXR mRNA in PTX only (PTX) or treated cells exposed to transfection conditions in the absence of siRNA (mock) or cells transfected with non-targeting (NC) or PXR-specific siRNA (siRNA). (D) Comparison of MDR1 mRNA expression in the cells exposed to transfection conditions with 8 h/36 h DOX and PTX treatment. **, p < 0.01 (n = 3). (E) Rho-123 fluorescence ratio in the cells exposed to transfection conditions with 8 h/36 h DOX and PTX treatment. *, p < 0.05 (n = 3).

Article Snippet: The siRNA sequence targeting human PXR gene and the negative control that consisted of a non-complementary sequence were developed by GenePharma (Shanghai, China).

Techniques: Activation Assay, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Transfection, Comparison, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: Apobec-1 Complementation Factor (A1CF) Inhibits Epithelial-Mesenchymal Transition and Migration of Normal Rat Kidney Proximal Tubular Epithelial Cells

doi: 10.3390/ijms17020197

Figure Lengend Snippet: Apobec-1 complementation factor (A1CF) is highly conserved among species. ( a ) A1CF amino acid sequences of human , mouse , rat , chicken , zebrafish , xenopus laevis were selected to analysis. The identical amino acids were shaded in black and the similar amino acids in grey; ( b ) Phylogenetic tree of A1CF amino acids is constructed using MEGA6 software, bootstrap percentages based on 1000 replicates, are shown on each branch. Identity values are listed on the right ranging from 78% to 92%, revealing that A1CF is highly conserved among species.

Article Snippet: SiRNA oligonucleotides were purchased from Shanghai GenePharma Co., Ltd. Two stealth siRNAs targeting different sequences of A1CF mRNA were selected to silence A1CF of Rattus norvegicus .

Techniques: Construct, Software

Journal: International Journal of Molecular Sciences

Article Title: Apobec-1 Complementation Factor (A1CF) Inhibits Epithelial-Mesenchymal Transition and Migration of Normal Rat Kidney Proximal Tubular Epithelial Cells

doi: 10.3390/ijms17020197

Figure Lengend Snippet: A1CF is expressed in mouse kidney starting at E15.5d and centers on the kidney tubules in C57BL/6 mice . ( a – c ) Whole mount in situ hybridization demonstrates that A1CF is expressed in the later stage of kidney development of C57BL/6 mice . White dot line indicates the area of metanephros rudiment (original magnification, ×40); ( c ′ ) To clearly prove the expression of A1CF, metanephros of embryonic day 15.5 (E15.5d) was detected with original magnification, ×100; ( d – g ) Section in situ hybridization demonstrates that in the adult mice kidney, A1CF is mainly expressed in the tubules of inner cortex but not in the other parts of the kidney section; ( d ) The black dot line marks the boundary between cortex and medulla. Scale bar represents 30 µm; ( e – g ) Images is the amplification of ( d ). ( e ) Scale bar represents 50 µm; ( f ) Image represents the field surrounding kidney tubules; Scale bar represents 100 µm; ( g ) Image represents the field of kidney tubules. Scale bar represents 100 µm; ( h , i ) In control groups no hybridization signal can be detected; ( h ) Scale bar represents 50 µm; ( i ) Scale bar represents 100 µm.

Article Snippet: SiRNA oligonucleotides were purchased from Shanghai GenePharma Co., Ltd. Two stealth siRNAs targeting different sequences of A1CF mRNA were selected to silence A1CF of Rattus norvegicus .

Techniques: In Situ Hybridization, Expressing, Amplification, Hybridization

Journal: International Journal of Molecular Sciences

Article Title: Apobec-1 Complementation Factor (A1CF) Inhibits Epithelial-Mesenchymal Transition and Migration of Normal Rat Kidney Proximal Tubular Epithelial Cells

doi: 10.3390/ijms17020197

Figure Lengend Snippet: Ectopic expression of A1CF inhibits EMT. ( a ) Overexpression A1CF and A1CF (-8AA) both result in gain of epithelial markers (E-cadherin) and loss of mesenchymal markers (vimentin and α-smooth muscle actin (α-SMA)), and the two variants have the similar trend in this process, GAPDH served as loading control; ( b ) Statistical analysis of relative protein expression levels. Data (mean ± standard error of the mean (S.E.M.)) are representative of three independent experiments. * p ≤ 0.05 and ** p ≤ 0.01 indicate significant statistical differences compared with the control group; ( c ) Immunofluorescence analysis demonstrates that ectopic expression of A1CF and A1CF (-8AA) potentiate the expression of membrane E-cadherin in NRK52e cells, and the expression of α-SMA and vimentin is weaker compared with control groups (original magnification, ×40). Scale bar represents 50 µm.

Article Snippet: SiRNA oligonucleotides were purchased from Shanghai GenePharma Co., Ltd. Two stealth siRNAs targeting different sequences of A1CF mRNA were selected to silence A1CF of Rattus norvegicus .

Techniques: Expressing, Over Expression, Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Apobec-1 Complementation Factor (A1CF) Inhibits Epithelial-Mesenchymal Transition and Migration of Normal Rat Kidney Proximal Tubular Epithelial Cells

doi: 10.3390/ijms17020197

Figure Lengend Snippet: A1CF knockdown induces EMT. ( a ) Control-siRNA, A1CF-siRNA #1, and A1CF-siRNA #2 were transfected into NRK52e cells. Western blotting analysis reveals a decrease in E-cadherin (epithelial marker) and concomitant increase in vimentin and α-SMA (mesenchymal markers). GAPDH served as loading control; ( b ) Statistical analysis of relative protein expression levels , Data (mean ± S.E.M.) are representative of three independent experiments. ** p ≤ 0.01 indicates highly statistically significant differences compared with control-siRNA group; ( c ) A1CF-siRNA #2 was transfected into NRK52e cells. A1CF knockdown attenuates the expression of membrane E-cadherin and potentiates the expression of α-SMA and vimentin demonstrated by immunofluorescence analysis (original magnification, ×40). Scale bar represents 50 µm.

Article Snippet: SiRNA oligonucleotides were purchased from Shanghai GenePharma Co., Ltd. Two stealth siRNAs targeting different sequences of A1CF mRNA were selected to silence A1CF of Rattus norvegicus .

Techniques: Transfection, Western Blot, Marker, Expressing, Immunofluorescence

Journal: International Journal of Molecular Sciences

Article Title: Apobec-1 Complementation Factor (A1CF) Inhibits Epithelial-Mesenchymal Transition and Migration of Normal Rat Kidney Proximal Tubular Epithelial Cells

doi: 10.3390/ijms17020197

Figure Lengend Snippet: A1CF inhibits NRK52e cells migration. ( a ) Both A1CF and A1CF (-8AA) significantly inhibited NRK52e cells migration. Data were collected at 12 and 24 h (original magnification, ×100); ( c ) A1CF knockdown significantly increases NRK52e cells migration. Data were collected at 12 and 24 h (original magnification, ×100); ( b , d ) Data are presented as mean ± S.E.M. of three independent experiments. Statistical analysis was performed using Student’s t -test. ** p < 0.01 indicated significantly statistical differences compared with control groups.

Article Snippet: SiRNA oligonucleotides were purchased from Shanghai GenePharma Co., Ltd. Two stealth siRNAs targeting different sequences of A1CF mRNA were selected to silence A1CF of Rattus norvegicus .

Techniques: Migration