Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on the survival of HT-29 cells. (A) Structure of taraxasterol. (B) HT-29 cells were treated with LPS, LPS+2.5 µg/ml taraxasterol, LPS+5 µg/ml taraxasterol or LPS+10 µg/ml taraxasterol. MTT assay was performed to assess cell viability. There was no differences between control and Drugs+LPS groups. *P<0.05 vs. control; # P<0.05 vs. Drugs+LPS groups. LPS, lipopolysaccharide.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: MTT Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on secretion of IL-6 and TNF-α. HT-29 cells were treated with 10 µg/ml LPS, then with 2.5, 5 or 10 µg/ml taraxasterol. The supernatants were collected to detect the expression levels of (A) IL-6 and (B) TNF-α. # P<0.05 vs. control; *P<0.05 vs. LPS only group. TNF-α, tumor necrosis factor alpha; IL-6, interleukin 6; LPS, lipopolysaccharide.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on apoptosis rate and protein expression of BAX, cleaved caspase-3 and p53. HT-29 cells were treated with 10 µg/ml LPS, then treated with 2.5, 5 or 10 mg/ml taraxasterol. (A) Cells were analyzed by flow cytometry and (B) the apoptosis rate (%) was calculated. (C) Representative images from western blot analysis of apoptosis-related proteins and quantification of protein expression levels of (D) BAX, (E) cleaved caspase-3 and (F) p53, as a ratio to β-actin. # P<0.05 vs. control; *P<0.05 vs. LPS only group. LPS, lipopolysaccharide; BAX, bcl-2-associated X protein; p53, tumor protein p53.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Flow Cytometry, Western Blot, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Colonic histopathological micrographs and p53 expression levels. Mice were randomly divided into 5 groups (n=12); control group, model group (DSS group) and low, medium and high dose (25, 50 and 100 mg/kg/day respectively) taraxasterol groups. (A) Colonic tissues from each group were stained with H&E (magnification, ×100). The red arrows indicate granulation tissue hyperplasia and glandular enlargement. (B) p53 expression levels (brown staining) were measured by immunohistochemistry assay (magnification, ×100). p53, tumor protein p53; DSS, dextran sodium sulphate; H&E, hematoxylin and eosin.

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, Control, Staining, Immunohistochemistry

Journal: Experimental and Therapeutic Medicine

Article Title: Anti-inflammatory and anti-apoptosis activity of taraxasterol in ulcerative colitis in vitro and in vivo

doi: 10.3892/etm.2019.7736

Figure Lengend Snippet: Effect of taraxasterol on histopathological score, p53 IHC score and expression of TNF-α and IL-6 in vivo .

Article Snippet: Taraxasterol (purity, 98% HPLC) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China).

Techniques: Expressing, In Vivo, Control

Journal: Infectious Agents and Cancer

Article Title: In vitro inhibition effects of hepatitis B virus by dandelion and taraxasterol

doi: 10.1186/s13027-020-00309-4

Figure Lengend Snippet: Chemimcal structure of taraxasterol ( a ). Cellular toxicity of dandelion and taraxasterol in HepG2.2.15 ( b and c ). After HepG2.2.15 cells were treated with dandelion and taraxasterol for 3 and 9 days, the cells were detected by MTT. The results represented the mean data from three independent experiments

Article Snippet: Dandelion extracts (batch number P-004) and taraxasterol (batch number; P-002, > 98% purity) were obtained from Chengdu Herbpurify Co., Ltd. A voucher specimen for T. officinale F.H.Wigg. (Compositae) was retained for future reference (Fig.S ).

Techniques:

Journal: Infectious Agents and Cancer

Article Title: In vitro inhibition effects of hepatitis B virus by dandelion and taraxasterol

doi: 10.1186/s13027-020-00309-4

Figure Lengend Snippet: Inhibition of HBV replication in HepG2.2.15 treated with taraxasterol. After HepG2.2.15 cells were treated with taraxasterol for 3 and 9 days, the culture supernatant was harvested. HBsAg and HBeAg were determined by ELISA assay, and HBV DNA was detected by qPCR. The results represent the mean data from three independent experiments. As compared to DMSO negative control group,* P < 0.05 and ** P < 0.01

Article Snippet: Dandelion extracts (batch number P-004) and taraxasterol (batch number; P-002, > 98% purity) were obtained from Chengdu Herbpurify Co., Ltd. A voucher specimen for T. officinale F.H.Wigg. (Compositae) was retained for future reference (Fig.S ).

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: Infectious Agents and Cancer

Article Title: In vitro inhibition effects of hepatitis B virus by dandelion and taraxasterol

doi: 10.1186/s13027-020-00309-4

Figure Lengend Snippet: Immunofluorescence of HBsAg in the control, dandelion (100 μg/mL) and taraxasterol (24 μg/mL) groups for 3d. a Cells were fixed and immunostained using monoclonal anti-HBsAg (green) antibody. Hoechst 33342 (blue) was used to counterstain nuclei. Representative images were shown. b HBsAg fluorescence intensity revealed the significantly decreased fluorescence intensity (green color) in dandelion and taraxasterol groups. As compared to DMSO negative control group,* P < 0.05

Article Snippet: Dandelion extracts (batch number P-004) and taraxasterol (batch number; P-002, > 98% purity) were obtained from Chengdu Herbpurify Co., Ltd. A voucher specimen for T. officinale F.H.Wigg. (Compositae) was retained for future reference (Fig.S ).

Techniques: Immunofluorescence, Control, Fluorescence, Negative Control

Journal: Infectious Agents and Cancer

Article Title: In vitro inhibition effects of hepatitis B virus by dandelion and taraxasterol

doi: 10.1186/s13027-020-00309-4

Figure Lengend Snippet: PTBP1 expression inhibited by taraxasterol in HepG2.2.15 cells. a HepG2.2.15 cells were treated with taraxasterol for 48 h. The expression levels of PTBP1 were detected by Western blot assay. b The images were quantified and shown. All results represented the mean ± SEM data from three independent experiments. As compared to the DMSO negative control group,* P < 0.05

Article Snippet: Dandelion extracts (batch number P-004) and taraxasterol (batch number; P-002, > 98% purity) were obtained from Chengdu Herbpurify Co., Ltd. A voucher specimen for T. officinale F.H.Wigg. (Compositae) was retained for future reference (Fig.S ).

Techniques: Expressing, Western Blot, Negative Control

Journal: Infectious Agents and Cancer

Article Title: In vitro inhibition effects of hepatitis B virus by dandelion and taraxasterol

doi: 10.1186/s13027-020-00309-4

Figure Lengend Snippet: Sirt1 expression inhibited by taraxasterol in HepG2.2.15 cells. a HepG2.2.15 cells were treated with taraxasterol for 48 h. The expression levels of Sirt1 were detected by Western blot assay. b The images were quantified and shown. All results represented the mean ± SEM data from three independent experiments. As compared to the DMSO negative control group,* P < 0.05

Article Snippet: Dandelion extracts (batch number P-004) and taraxasterol (batch number; P-002, > 98% purity) were obtained from Chengdu Herbpurify Co., Ltd. A voucher specimen for T. officinale F.H.Wigg. (Compositae) was retained for future reference (Fig.S ).

Techniques: Expressing, Western Blot, Negative Control

Journal: Computational and Structural Biotechnology Journal

Article Title: Inhibition of the main protease of SARS-CoV-2 (M pro ) by repurposing/designing drug-like substances and utilizing nature’s toolbox of bioactive compounds

doi: 10.1016/j.csbj.2022.03.009

Figure Lengend Snippet: Natural compounds highlighted in molecular docking studies for their potential inhibitory effect against SARS-CoV-2 M pro .

Article Snippet: Psi-taraxasterol , - , Autodock Vina , 6LU7 , -8.5 , Met49, Cys145, Met165 , , .

Techniques: Software, Binding Assay