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  • 99
    Thermo Fisher taqman advanced mirna assays chemistry
    Taqman Advanced Mirna Assays Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman chemistry
    Patterns of cytokine expression in active and inactive periapical granulomas. Total RNA was extracted from periapical granulomas (N=110) and periodontal ligament control samples (N=26), and levels of TNF-α(classic pro-inflammmatory cytokine), IL-10 (Treg and Tr1 marker), IFN-γ (Th1 marker), IL-4 (Th2 marker), <t>FOXp3</t> (Treg marker), CTLA4 (Treg marker), TGF-β (Treg and Th3 marker), IL-9 (Th9 marker), IL-17A (Th17 marker), IL-17F (Th17 marker), IL-21 (Th17 or Tfh marker), IL-23 (Th17 marker) and IL-22 (Th22 marker) were measured quantitatively by RealTimePCR using <t>TaqMan</t> chemistry. Based on the profile of RANKL/OPG expression29, the lesions were then categorized into active (RANKL > OPG) or inactive (RANKL≈OPG and RANKL
    Taqman Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2818 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman probe based chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Taqman Probe Based Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman 5 fluorogenic nuclease chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
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    Thermo Fisher taqman mgb chemistry
    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by <t>qRT-PCR</t> using <t>Taqman</t> probes (white symbols).
    Taqman Mgb Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman chemistries
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Chemistries, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher abi taqman chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Abi Taqman Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman chemistry based allelic discrimination assay
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Chemistry Based Allelic Discrimination Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman gene expression master mix
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Gene Expression Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman fast virus 1 step master mix chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Fast Virus 1 Step Master Mix Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman assay chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Assay Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman allelic discrimination chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Allelic Discrimination Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman mgb probe chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Mgb Probe Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman qpcr chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Qpcr Chemistry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman chemistry pcr system
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Chemistry Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman chemistry genotyping system
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Chemistry Genotyping System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PrimerDesign Inc taqman chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
    Taqman Chemistry, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taqman fast advanced chemistry
    GPR35 is expressed in human VSMCs and ECs. <t>hGPR35</t> mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via <t>TaqMan.</t> HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.
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    Image Search Results


    Patterns of cytokine expression in active and inactive periapical granulomas. Total RNA was extracted from periapical granulomas (N=110) and periodontal ligament control samples (N=26), and levels of TNF-α(classic pro-inflammmatory cytokine), IL-10 (Treg and Tr1 marker), IFN-γ (Th1 marker), IL-4 (Th2 marker), FOXp3 (Treg marker), CTLA4 (Treg marker), TGF-β (Treg and Th3 marker), IL-9 (Th9 marker), IL-17A (Th17 marker), IL-17F (Th17 marker), IL-21 (Th17 or Tfh marker), IL-23 (Th17 marker) and IL-22 (Th22 marker) were measured quantitatively by RealTimePCR using TaqMan chemistry. Based on the profile of RANKL/OPG expression29, the lesions were then categorized into active (RANKL > OPG) or inactive (RANKL≈OPG and RANKL

    Journal: Journal of Applied Oral Science

    Article Title: Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status

    doi: 10.1590/1678-775720140140

    Figure Lengend Snippet: Patterns of cytokine expression in active and inactive periapical granulomas. Total RNA was extracted from periapical granulomas (N=110) and periodontal ligament control samples (N=26), and levels of TNF-α(classic pro-inflammmatory cytokine), IL-10 (Treg and Tr1 marker), IFN-γ (Th1 marker), IL-4 (Th2 marker), FOXp3 (Treg marker), CTLA4 (Treg marker), TGF-β (Treg and Th3 marker), IL-9 (Th9 marker), IL-17A (Th17 marker), IL-17F (Th17 marker), IL-21 (Th17 or Tfh marker), IL-23 (Th17 marker) and IL-22 (Th22 marker) were measured quantitatively by RealTimePCR using TaqMan chemistry. Based on the profile of RANKL/OPG expression29, the lesions were then categorized into active (RANKL > OPG) or inactive (RANKL≈OPG and RANKL

    Article Snippet: All cytokines/Th markers (TNF-α, IFN-γ, IL-17A, IL-23, IL-21, IL-33, IL-10, IL-4, IL-9, IL-22, FOXp3) mRNA levels were measured by means of RealTimePCR using TaqMan chemistry (Invitrogen, Carlsbad, CA) in a Viia7 instrument (LifeTechnologies, Carlsbad, CA) using inventoried optimized primers/probes sets (Invitrogen, Carlsbad, CA), with basic reaction conditions (40 cycles) at 95ºC (10'), 94ºC (1'), 56ºC (1') and 72ºC (2').

    Techniques: Expressing, Marker

    Expression of individual mRNAs, with normalization to housekeeping genes, in periapical granulomas. Total RNA was extracted from periapical granulomas (experimental groups, N=110) and periodontal ligament (control group, N=26), and levels of RANKL and OPG mRNA were measured quantitatively by RealTimePCR using TaqMan chemistry. Based in profile of RANKL/OPG expression29 the lesions were then categorized into active (RANKL > OPG) or inactive (RANKL»OPG and RANKL

    Journal: Journal of Applied Oral Science

    Article Title: Simultaneous analysis of T helper subsets (Th1, Th2, Th9, Th17, Th22, Tfh, Tr1 and Tregs) markers expression in periapical lesions reveals multiple cytokine clusters accountable for lesions activity and inactivity status

    doi: 10.1590/1678-775720140140

    Figure Lengend Snippet: Expression of individual mRNAs, with normalization to housekeeping genes, in periapical granulomas. Total RNA was extracted from periapical granulomas (experimental groups, N=110) and periodontal ligament (control group, N=26), and levels of RANKL and OPG mRNA were measured quantitatively by RealTimePCR using TaqMan chemistry. Based in profile of RANKL/OPG expression29 the lesions were then categorized into active (RANKL > OPG) or inactive (RANKL»OPG and RANKL

    Article Snippet: All cytokines/Th markers (TNF-α, IFN-γ, IL-17A, IL-23, IL-21, IL-33, IL-10, IL-4, IL-9, IL-22, FOXp3) mRNA levels were measured by means of RealTimePCR using TaqMan chemistry (Invitrogen, Carlsbad, CA) in a Viia7 instrument (LifeTechnologies, Carlsbad, CA) using inventoried optimized primers/probes sets (Invitrogen, Carlsbad, CA), with basic reaction conditions (40 cycles) at 95ºC (10'), 94ºC (1'), 56ºC (1') and 72ºC (2').

    Techniques: Expressing

    Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by qRT-PCR using Taqman probes (white symbols).

    Journal: BMC Genomics

    Article Title: Highlights of glycosylation and adhesion related genes involved in myogenesis

    doi: 10.1186/1471-2164-15-621

    Figure Lengend Snippet: Myogenic differentiation of C2C12 with or without Matrigel®. The C2C12 cells were seeded at 5x10 3 cells/cm 2 on plates coated (square) or not (triangle) with Matrigel®. The fusion index was measured every 24 hours after differentiation induction by serum starvation (black symbols), vertical bars correspond to standard errors (n = 3). Relative mRNA quantities of MyoG (solid line) or Itgb8 (dotted line) were also measured every 24 hours by qRT-PCR using Taqman probes (white symbols).

    Article Snippet: A micro-fluidic chip was used to measure quality and quantity of total RNA (Agilent 2100 Bioanalyser, Agilent Technologies Inc., Santa Clara, CA, USA) and 1 μg was converted into cDNA using the High Capacity cDNA Archive Kit (Applied Biosystems, Foster city, CA, USA). mRNA was quantified by QRT-PCR on ABI Prism 7900 Sequence Detector System using TaqMan probe-based chemistry (Applied Biosystems), with 6-carboxyfluorescein (FAM) as a reporter. cDNA (2 ng) was used to quantify myogenic and adipogenic markers and target genes in 96-well plates and Taqman Low Density Array (TLDA) respectively.

    Techniques: Quantitative RT-PCR

    Expression changes (mRNA) of evaluation genes. A-C : Relative mRNA concentrations in the mouse neural retina were measured by qPCR (real time) for the developmental ages P2 and P25. Concentrations were normalized to the beta-Actin mRNA concentration. Bars indicate standard deviation for triplicate assays. Taqman chemistry was used for target specificity with hydrolysis probes that span exon junctions. Rho , Rcvn , Pde6b, and Sag1 are key markers of photoreceptor-specific gene expression. Genes are grouped to account for different scales of relative expression. Most genes had Pol-II peak signal ratios > 1.8, as determined from temporal Pol-II ChIP-on-Chip analysis, except for: Hdac9 (ratio 1.6), Hdac10 (ratio 1.7), and Grik2 (ratio 0.9, Table 1 ). Genes: Rhodopsin (Rho), Recoverin (Rcvrn), Retinoschisis 1 (Rs1), Phosphodiesterase 6b (Pde6b), S-antigen (Sag), Elongation of very long chain fatty acids-like 4 (Elovl4), D4, zinc and double PHD fingers, family 3 (Dpf3), Spectrin repeat containing, nuclear envelope 1 (Syne1), Solute carrier family 38, Na/H -coupled glutamine transporter, member 3 (Slc38a3), Family with sequence similarity 53, member B (A930008G19Rik, Fam53b), Nuclear receptor subfamily 1, group D, member 1 (Nr1d1), Jumonji domain containing 2C (Jmjd2c), Histone deacetylase 9 (Hdac9), Bardet-Biedl syndrome 9 (E130103I17Rik, Bbs9), Histone deacetylase 10 (Hdac10), Glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), Solute carrier family 24, Na/K/Ca exchanger, member 1 (Slc24a1).

    Journal: Molecular Vision

    Article Title: Temporal ChIP-on-Chip of RNA-Polymerase-II to detect novel gene activation events during photoreceptor maturation

    doi:

    Figure Lengend Snippet: Expression changes (mRNA) of evaluation genes. A-C : Relative mRNA concentrations in the mouse neural retina were measured by qPCR (real time) for the developmental ages P2 and P25. Concentrations were normalized to the beta-Actin mRNA concentration. Bars indicate standard deviation for triplicate assays. Taqman chemistry was used for target specificity with hydrolysis probes that span exon junctions. Rho , Rcvn , Pde6b, and Sag1 are key markers of photoreceptor-specific gene expression. Genes are grouped to account for different scales of relative expression. Most genes had Pol-II peak signal ratios > 1.8, as determined from temporal Pol-II ChIP-on-Chip analysis, except for: Hdac9 (ratio 1.6), Hdac10 (ratio 1.7), and Grik2 (ratio 0.9, Table 1 ). Genes: Rhodopsin (Rho), Recoverin (Rcvrn), Retinoschisis 1 (Rs1), Phosphodiesterase 6b (Pde6b), S-antigen (Sag), Elongation of very long chain fatty acids-like 4 (Elovl4), D4, zinc and double PHD fingers, family 3 (Dpf3), Spectrin repeat containing, nuclear envelope 1 (Syne1), Solute carrier family 38, Na/H -coupled glutamine transporter, member 3 (Slc38a3), Family with sequence similarity 53, member B (A930008G19Rik, Fam53b), Nuclear receptor subfamily 1, group D, member 1 (Nr1d1), Jumonji domain containing 2C (Jmjd2c), Histone deacetylase 9 (Hdac9), Bardet-Biedl syndrome 9 (E130103I17Rik, Bbs9), Histone deacetylase 10 (Hdac10), Glutamate receptor, ionotropic, kainate 2 (beta 2) (Grik2), Solute carrier family 24, Na/K/Ca exchanger, member 1 (Slc24a1).

    Article Snippet: Gene expression assays—real-time PCR Specific genes from the ChIP-on-chip data representing a full range of Pol-II peak signal ratios (P25/P2) were analyzed by real-time PCR, using Taqman probe chemistry following the manufacturer’s standard protocol (Applied Biosystems, Foster City, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Standard Deviation, Chromatin Immunoprecipitation, Sequencing, Histone Deacetylase Assay

    GPR35 is expressed in human VSMCs and ECs. hGPR35 mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via TaqMan. HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.

    Journal: Journal of Vascular Research

    Article Title: G-Protein-Coupled Receptor 35 Mediates Human Saphenous Vein Vascular Smooth Muscle Cell Migration and Endothelial Cell Proliferation

    doi: 10.1159/000444754

    Figure Lengend Snippet: GPR35 is expressed in human VSMCs and ECs. hGPR35 mRNA expression in human tissues and HSV ECs (n = 3) normalized to 18S and expressed as RQ relative to hGPR35 expression in the heart ( a ) or in a range of immortalized and primary cells ( b ) was quantified via TaqMan. HSV ECs (n = 3), and SMCs (n = 3), HUVECs (n = 3) and HT-29 colon carcinoma cells are expressed as copy numbers per nanogram of RNA. Data are shown as means ± SEM.

    Article Snippet: Reverse-transcriptase reactions were carried out using a Taqman Multiscribe RT kit with random hexamers according to the manufacturer's instructions. mRNA expression of hGPR35 and ribosomal 18S were quantified by real-time PCR using Taqman chemistries (Applied Biosystems, Warrington, UK).

    Techniques: Expressing