taq ligase New England Biolabs Search Results


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  • 90
    New England Biolabs taq ligase
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Taq Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    New England Biolabs hifi taq dna ligase
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Hifi Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 8 article reviews
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    95
    New England Biolabs ligase reaction buffer
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Ligase Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 46 article reviews
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    84
    New England Biolabs taq ligase buffer
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Taq Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 84 stars, based on 21 article reviews
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    89
    New England Biolabs cohesive end taq ligase
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Cohesive End Taq Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs longamp taq reaction buffer
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Longamp Taq Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs longamp taq
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Longamp Taq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs taq dna polymerase with thermopol buffer
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Taq Dna Polymerase With Thermopol Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 252 article reviews
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    95
    New England Biolabs long amp taq 2x master mix
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Long Amp Taq 2x Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    New England Biolabs taq ligation buffer
    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) <t>taq</t> DNA ligase, and (c) <t>ampligase.</t>
    Taq Ligation Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs taq dna polymerase
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 8653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 8653 article reviews
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    taq dna polymerase - by Bioz Stars, 2020-02
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    79
    New England Biolabs go taq buffer
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    Go Taq Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    go taq buffer - by Bioz Stars, 2020-02
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    95
    New England Biolabs hot start dna polymerase
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    Hot Start Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 34 article reviews
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    hot start dna polymerase - by Bioz Stars, 2020-02
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    95
    New England Biolabs longamp taq dna polymerase
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    Longamp Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 749 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    New England Biolabs 10×taq dna ligase buffer
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    10×Taq Dna Ligase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10×taq dna ligase buffer/product/New England Biolabs
    Average 88 stars, based on 10 article reviews
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    10×taq dna ligase buffer - by Bioz Stars, 2020-02
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    95
    New England Biolabs one taq hot start 2x pcr master mix
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    One Taq Hot Start 2x Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 11 article reviews
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    99
    New England Biolabs phusion taq polymerase
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    Phusion Taq Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs platinum taq dna polymerase high fidelity
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    Platinum Taq Dna Polymerase High Fidelity, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs dna modifying enzymes
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    Dna Modifying Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2011 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 2011 article reviews
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    95
    New England Biolabs high fidelity taq dna polymerase
    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe <t>Taq</t> ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured <t>DNA.</t> ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).
    High Fidelity Taq Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 11 article reviews
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    Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) taq DNA ligase, and (c) ampligase.

    Journal: BioMed Research International

    Article Title: Detection of Food Spoilage and Pathogenic Bacteria Based on Ligation Detection Reaction Coupled to Flow-Through Hybridization on Membranes

    doi: 10.1155/2014/156323

    Figure Lengend Snippet: Biodyne C membrane-macroarray images obtained for optimization experiments with biotin labeling and different ligase enzymes: (a) pfu DNA ligase, (b) taq DNA ligase, and (c) ampligase.

    Article Snippet: Three ligase enzymes were tested: pfu DNA ligase (Stratagene, La Jolla, CA, USA), ampligase (Epicentre, Madison, WI, USA), and taq ligase (New England Biolabs, Beverly, MA, USA).

    Techniques: Labeling

    PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe Taq ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured DNA. ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Generating a synthetic genome by whole genome assembly: ?X174 bacteriophage from synthetic oligonucleotides

    doi: 10.1073/pnas.2237126100

    Figure Lengend Snippet: PCA of full-length synφX molecules. The first stage of PCA (50-μl reaction volume) was carried out for 35 cycles with 0.2, 0.5, or 1 μlofthe Taq ligation product. PCA products were analyzed on a 0.8% E-gel. ( A ) Lanes 1 and 6, 1-kb ladder; lane 2, 0.5 μlof Taq ligation product; lane 3, 2 μl of the 0.2-μl PCA; lane 4, 2 μl of the 0.5-μl PCA; lane 5, 2 μl of the 1-μl PCA. The second stage of PCA was for an additional 35 cycles in five new 50-μl reactions. For reaction 1, the 0.2-μl first-stage reaction was continued without change for another 35 cycles with the addition of 0.5 μl of fresh HF polymerase mixture. For reactions 2 and 3, 10 and 20 μl of the 0.5-μl first-stage PCA product was used. For reactions 4 and 5, 5 and 10 μl of the 1-μl first-stage PCA product was used. Analysis was on 0.8% E-gels. ( B ) Formamide-denatured DNA. ( C ) Native DNA. ( B and C ) Lanes 1 and 7, 1-kb ladder; lane 2, 2 μl of reaction 1; lanes 3 and 4, 2 μl of reactions 2 and 3; lanes 5 and 6, 2 μl of reactions 4 and 5. ( D ) PCR amplification of the products of the second set of PCA products as shown in B and C .( E ) Taq ligase assembly of 259 oligonucleotides. A 0.5-μl sample of the ligation products was analyzed on a 2% E-gel (Invitrogen) in duplex form (lane N). One microliter of the ligation products was mixed with 20 μl of formamide, heated to 95°C for 2 min, and then analyzed (lane D). Denatured standards run approximately the same as native standards, based on other experiments (data not shown).

    Article Snippet: The polymerase mixture contains both an N-terminal deletion mutant of Taq DNA polymerase that lacks 5′-exonuclease activity and Deep VentR polymerase (NEB) with 3′-exonuclease proofreading activity.

    Techniques: Ligation, Polymerase Chain Reaction, Amplification