taq buffer Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher taq buffer
    Amplification directly from blood. A 322 bp IGF target was amplified from 5 to 25 μl EDTA-blood using 50 ng of each mutant <t>Taq</t> <t>DNA</t> polymerase. Reactions were cycled on the SureCycler 8800 using the following parameters: 5 min at 90°C, followed by 30 cycles of 30 s at 95°C, 30 s at 60°C, 60 s at 72°C. Wild-type Taq can amplify the 322 bp from human genomic DNA in the absence of blood, but not in the presence of 1% blood (data not shown), even though the amount of EDTA introduced with 0.5 μl EDTA-blood (0.089 mM EDTA) is well below inhibitory levels.
    Taq Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq buffer/product/Thermo Fisher
    Average 99 stars, based on 3124 article reviews
    Price from $9.99 to $1999.99
    taq buffer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher taq
    Amplification directly from blood. A 322 bp IGF target was amplified from 5 to 25 μl EDTA-blood using 50 ng of each mutant <t>Taq</t> <t>DNA</t> polymerase. Reactions were cycled on the SureCycler 8800 using the following parameters: 5 min at 90°C, followed by 30 cycles of 30 s at 95°C, 30 s at 60°C, 60 s at 72°C. Wild-type Taq can amplify the 322 bp from human genomic DNA in the absence of blood, but not in the presence of 1% blood (data not shown), even though the amount of EDTA introduced with 0.5 μl EDTA-blood (0.089 mM EDTA) is well below inhibitory levels.
    Taq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 2754 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq/product/Thermo Fisher
    Average 95 stars, based on 2754 article reviews
    Price from $9.99 to $1999.99
    taq - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    Image Search Results


    Amplification directly from blood. A 322 bp IGF target was amplified from 5 to 25 μl EDTA-blood using 50 ng of each mutant Taq DNA polymerase. Reactions were cycled on the SureCycler 8800 using the following parameters: 5 min at 90°C, followed by 30 cycles of 30 s at 95°C, 30 s at 60°C, 60 s at 72°C. Wild-type Taq can amplify the 322 bp from human genomic DNA in the absence of blood, but not in the presence of 1% blood (data not shown), even though the amount of EDTA introduced with 0.5 μl EDTA-blood (0.089 mM EDTA) is well below inhibitory levels.

    Journal: Frontiers in Microbiology

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

    doi: 10.3389/fmicb.2014.00408

    Figure Lengend Snippet: Amplification directly from blood. A 322 bp IGF target was amplified from 5 to 25 μl EDTA-blood using 50 ng of each mutant Taq DNA polymerase. Reactions were cycled on the SureCycler 8800 using the following parameters: 5 min at 90°C, followed by 30 cycles of 30 s at 95°C, 30 s at 60°C, 60 s at 72°C. Wild-type Taq can amplify the 322 bp from human genomic DNA in the absence of blood, but not in the presence of 1% blood (data not shown), even though the amount of EDTA introduced with 0.5 μl EDTA-blood (0.089 mM EDTA) is well below inhibitory levels.

    Article Snippet: The radiolabeled hairpin (0.25 nM) was incubated with varying concentrations of wild-type or mutant Taq DNA polymerases (0.5–1000 nM; bracketing the predicted Kd ) in 1× Taq buffer at 37°C for 30 min. DNA-protein complexes were run on 10% non-denaturing TBE gels (Life Technologies) in 0.5× TBE buffer.

    Techniques: Amplification, Mutagenesis

    Fast qPCR assays employing SYBR Green. Genomic DNA targets were amplified as described in Methods using 0.5 ng human gDNA and 10 ng of purified wild-type or mutant Taq DNA polymerase. Reactions were cycled on the StepOnePlus instrument (Life Technologies) using cycling conditions consisting of 3 min at 95°C followed by 40 cycles of 3 s at 95°C, 10 s at 60°C. Genomic DNA targets are as follows: (A) 91 bp ABC, (B) 109 bp COMTE2, (C) 305 bp Numb. Sixty second extension time is required for Taq wild-type to efficiently amplify the 305 bp target (data not shown).

    Journal: Frontiers in Microbiology

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

    doi: 10.3389/fmicb.2014.00408

    Figure Lengend Snippet: Fast qPCR assays employing SYBR Green. Genomic DNA targets were amplified as described in Methods using 0.5 ng human gDNA and 10 ng of purified wild-type or mutant Taq DNA polymerase. Reactions were cycled on the StepOnePlus instrument (Life Technologies) using cycling conditions consisting of 3 min at 95°C followed by 40 cycles of 3 s at 95°C, 10 s at 60°C. Genomic DNA targets are as follows: (A) 91 bp ABC, (B) 109 bp COMTE2, (C) 305 bp Numb. Sixty second extension time is required for Taq wild-type to efficiently amplify the 305 bp target (data not shown).

    Article Snippet: The radiolabeled hairpin (0.25 nM) was incubated with varying concentrations of wild-type or mutant Taq DNA polymerases (0.5–1000 nM; bracketing the predicted Kd ) in 1× Taq buffer at 37°C for 30 min. DNA-protein complexes were run on 10% non-denaturing TBE gels (Life Technologies) in 0.5× TBE buffer.

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Amplification, Purification, Mutagenesis

    Fast cycling with TaqMan detection. An Assays-on-Demand assay (Life Technologies; 171 bp β-actin target) was performed using 50, 5, 0.5 ng human genomic DNA and 10 ng of purified wild-type or mutant Taq DNA polymerase. Reactions were cycled on the StepOnePlus instrument using cycling conditions consisting of 2 min at 95°C followed by 40 cycles of: (A) 10 s at 95°C, 60 s at 60°C; or (B) 10 s at 95°C, 10 s at 60°C.

    Journal: Frontiers in Microbiology

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

    doi: 10.3389/fmicb.2014.00408

    Figure Lengend Snippet: Fast cycling with TaqMan detection. An Assays-on-Demand assay (Life Technologies; 171 bp β-actin target) was performed using 50, 5, 0.5 ng human genomic DNA and 10 ng of purified wild-type or mutant Taq DNA polymerase. Reactions were cycled on the StepOnePlus instrument using cycling conditions consisting of 2 min at 95°C followed by 40 cycles of: (A) 10 s at 95°C, 60 s at 60°C; or (B) 10 s at 95°C, 10 s at 60°C.

    Article Snippet: The radiolabeled hairpin (0.25 nM) was incubated with varying concentrations of wild-type or mutant Taq DNA polymerases (0.5–1000 nM; bracketing the predicted Kd ) in 1× Taq buffer at 37°C for 30 min. DNA-protein complexes were run on 10% non-denaturing TBE gels (Life Technologies) in 0.5× TBE buffer.

    Techniques: Purification, Mutagenesis

    Fast cycling conferred by E507K mutation. Genomic DNA targets were amplified as described in Methods using 10 ng human gDNA and 20 ng of purified wild-type or mutant Taq DNA polymerase. Reactions were cycled on the CFX96 instrument (BioRad) using cycling conditions consisting of 3 min at 95°C followed by 40 cycles of (left panels) 10 s at 95°C, 1 s at 60°C; or (right panels) 10 s at 95°C, 60 s at 60°C. Genomic DNA targets are as follows: (A) 286 bp Aldolase; and (B) 232 bp Quantos.

    Journal: Frontiers in Microbiology

    Article Title: Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance

    doi: 10.3389/fmicb.2014.00408

    Figure Lengend Snippet: Fast cycling conferred by E507K mutation. Genomic DNA targets were amplified as described in Methods using 10 ng human gDNA and 20 ng of purified wild-type or mutant Taq DNA polymerase. Reactions were cycled on the CFX96 instrument (BioRad) using cycling conditions consisting of 3 min at 95°C followed by 40 cycles of (left panels) 10 s at 95°C, 1 s at 60°C; or (right panels) 10 s at 95°C, 60 s at 60°C. Genomic DNA targets are as follows: (A) 286 bp Aldolase; and (B) 232 bp Quantos.

    Article Snippet: The radiolabeled hairpin (0.25 nM) was incubated with varying concentrations of wild-type or mutant Taq DNA polymerases (0.5–1000 nM; bracketing the predicted Kd ) in 1× Taq buffer at 37°C for 30 min. DNA-protein complexes were run on 10% non-denaturing TBE gels (Life Technologies) in 0.5× TBE buffer.

    Techniques: Mutagenesis, Amplification, Purification

    Reportable range for T. cruzi and triatomine intestine unit quantification by real-time qPCR. Multiplex Taq Man qPCR assays were carried out with serially diluted DNA extracted from reconstituted triatomine intestine samples containing T. cruzi epimastigotes, ranging from 10 5 to 0.5 T. cruzi equivalents ( a ) and 5 to 0.002 triatomine intestine unit equivalents ( b ). The slope, R 2 and amplification efficiency (Eff) are indicated in the chart

    Journal: Parasites & Vectors

    Article Title: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors

    doi: 10.1186/s13071-017-2343-x

    Figure Lengend Snippet: Reportable range for T. cruzi and triatomine intestine unit quantification by real-time qPCR. Multiplex Taq Man qPCR assays were carried out with serially diluted DNA extracted from reconstituted triatomine intestine samples containing T. cruzi epimastigotes, ranging from 10 5 to 0.5 T. cruzi equivalents ( a ) and 5 to 0.002 triatomine intestine unit equivalents ( b ). The slope, R 2 and amplification efficiency (Eff) are indicated in the chart

    Article Snippet: Multiplex conventional PCR (cPCR) Conventional PCR assays were carried out in a final volume of 50 μl, containing: 5 μl DNA (20–25 ng), 5 μl 10× Taq Platinum buffer, 0.2 mM dNTPs, 4.5 mM MgCl2 , 1.25 U Taq Platinum DNA polymerase (Life Technologies, Carlsbad, CA, USA), 200 nM 121/122 primers (T. cruzi kDNA) [ , ] and 100 nM P2B/P6R primers (triatomine 12S rRNA gene).

    Techniques: Real-time Polymerase Chain Reaction, Multiplex Assay, Amplification