Journal: Oncotarget

Article Title: The enhanced susceptibility of ADAM-17 hypomorphic mice to DSS-induced colitis is not ameliorated by loss of RIPK3, revealing an unexpected function of ADAM-17 in necroptosis

doi: 10.18632/oncotarget.24410

Figure Lengend Snippet: (A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM TAPI-1 (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).

Article Snippet: Highly purified human recombinant TNF was provided by BASF Bioresearch (Ludwigshafen, Germany). zVAD-fmk was from Bachem (Bubendorf, Switzerland), GW280264X from Iris Biotech (Marktredwitz, Germany), marimastat from BioTechne and GI254023X and TAPI-1 from Merck.

Techniques: Membrane, Marker, Western Blot, Two Tailed Test

Journal: Oral oncology

Article Title: P2Y 2 receptors mediate nucleotide-induced EGFR phosphorylation and stimulate proliferation and tumorigenesis of head and neck squamous cell carcinoma cell lines

doi: 10.1016/j.oraloncology.2020.104808

Figure Lengend Snippet: Selective inhibition of P2Y2R, ADAM10/17, EGFR and Src kinase diminishes UTP-induced EGFR phosphorylation. Representative immunoblots of CAL27 cells pretreated for 1 h with or without (A) AR-C118925 (10 μM), (B) TAPI-2 (10 μM), (C) cetuximab (10 μg/ml) or (D) dasatinib (100 nM) followed by 100 μM UTP or 10 ng/ml EGF for 1 min, then subjected to immunoblot analysis using antibodies for phospho-EGFR (Tyr1173) or total EGFR as a loading control. (E) UTP- or (F) EGF-induced EGFR (Tyr1173) phosphorylation levels were quantified and data are presented as the percentage of maximal EGFR phosphorylation induced by UTP or EGF alone. Data are presented as means ± S.E.M. for at least n = 3 experiments, where ** indicates P < 0.01, *** indicates P < 0.001 and **** indicates P < 0.0001.

Article Snippet: AR-C118925, AG1478, {"type":"entrez-nucleotide","attrs":{"text":"GF109203","term_id":"295317075","term_text":"GF109203"}} GF109203 and TAPI-2 were purchased from Bio-Techne Corporation (Minneapolis, MN).

Techniques: Inhibition, Western Blot

Journal: Bioscience Reports

Article Title: Sex differences in the lung ACE/ACE2 balance in hypertensive rats

doi: 10.1042/bsr20211201

Figure Lengend Snippet: Figure 4. Lung ADAM17 activity and its correlation with lung ACE2 protein and serum abundance in male and female

Article Snippet: Briefly, 5 μg of lung membrane proteins were incubated with reaction buffer (50 mM acetic acid, pH 4.5, 100 mM sodium chloride) in the presence or absence of the ADAM17 inhibitor TAPI 0 (10 μM; Tocris Biosciences, Minneapolis, MN, U.S.A.).

Techniques: Activity Assay

Journal: iScience

Article Title: Heightened innate immune state induced by viral vector leads to enhanced response to challenge and prolongs malaria vaccine protection

doi: 10.1016/j.isci.2024.111468

Figure Lengend Snippet:

Article Snippet: TAPI2 , Biosynth , Cat #INH-3852-PI.

Techniques: Control, Virus, Recombinant, Giemsa Stain, Cell Stimulation, Transfection, Software