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  • 99
    Millipore tandem mass spectrometry ms ms
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry Ms Ms, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Cyprotex Discovery tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry, supplied by Cyprotex Discovery, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Mayo Medical Laboratories tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry, supplied by Mayo Medical Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shimadzu Corporation tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Shimadzu Corporation chromatography mass spectrometry tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Chromatography Mass Spectrometry Tandem Mass Spectrometry, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 86/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    SCIEX tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry, supplied by SCIEX, used in various techniques. Bioz Stars score: 89/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore chromatography tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Chromatography Tandem Mass Spectrometry, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher tandem mass spectrometry ms ms
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry Ms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bioanalytical Systems tandem mass spectrometry
    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by <t>electrospray</t> ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.
    Tandem Mass Spectrometry, supplied by Bioanalytical Systems, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation tandem mass spectrometry ms ms
    Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in negative ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in DF1 (A). Lipid assignments based on <t>tandem</t> <t>mass</t> <t>spectrometry</t> <t>(MS/MS)</t> identified changes in the phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ), or the individual fatty acids and their respective position (sn 1 /sn 2 ).
    Tandem Mass Spectrometry Ms Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Waters Corporation electrospray tandem mass spectrometry
    Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in negative ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in DF1 (A). Lipid assignments based on <t>tandem</t> <t>mass</t> <t>spectrometry</t> <t>(MS/MS)</t> identified changes in the phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ), or the individual fatty acids and their respective position (sn 1 /sn 2 ).
    Electrospray Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation flight tandem mass spectrometry
    Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in negative ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in DF1 (A). Lipid assignments based on <t>tandem</t> <t>mass</t> <t>spectrometry</t> <t>(MS/MS)</t> identified changes in the phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ), or the individual fatty acids and their respective position (sn 1 /sn 2 ).
    Flight Tandem Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biocrates liquid chromatography lc tandem mass spectrometry tandem mass spectrometry ms ms assay
    Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in negative ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in DF1 (A). Lipid assignments based on <t>tandem</t> <t>mass</t> <t>spectrometry</t> <t>(MS/MS)</t> identified changes in the phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ), or the individual fatty acids and their respective position (sn 1 /sn 2 ).
    Liquid Chromatography Lc Tandem Mass Spectrometry Tandem Mass Spectrometry Ms Ms Assay, supplied by Biocrates, used in various techniques. Bioz Stars score: 90/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies tandem mass spectrometry gc ms ms
    Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in negative ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in DF1 (A). Lipid assignments based on <t>tandem</t> <t>mass</t> <t>spectrometry</t> <t>(MS/MS)</t> identified changes in the phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ), or the individual fatty acids and their respective position (sn 1 /sn 2 ).
    Tandem Mass Spectrometry Gc Ms Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by electrospray ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.

    Journal: Journal of Virology

    Article Title: The VP1 Unique Region of Parvovirus B19 and Its Constituent Phospholipase A2-Like Activity

    doi: 10.1128/JVI.76.4.2014-2018.2002

    Figure Lengend Snippet: ). The dried lipid extracts were dissolved in methanol-chloroform (3:1 [vol/vol]) containing 10 mM ammonium formate and characterized by electrospray ionization tandem mass spectrometry. We used a Quattro LC triple-quadrupole mass spectrometer (Micromass, Manchester, United Kingdom) with the following settings: capillary, 3.5 kV; cone, 41 V; collision energy, 24 V; and collision gas pressure, 1.3 10 −3 torr. Samples were injected at a constant flow rate of 75 μl/min with the Waters (Milford, Mass.) Alliance model 2790 filtration system and analyzed by a parent scan of m/z ). The intensity of the ratio of hydrolyzed product (LPC) to substrate (PC) was used to determine the degree of phospholipase A2 activity. Standard deviations are indicated by bars.

    Article Snippet: Analysis by electrospray ionization tandem mass spectrometry revealed exclusive γ-stearoyl-lysophosphatidylcholine (LPC) as the cleavage product for both the VP1 unique region and the bee venom phospholipase A2 (Sigma-Aldrich) that was used for standardization and comparison.

    Techniques: Mass Spectrometry, Injection, Flow Cytometry, Filtration, Activity Assay

    Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in negative ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in DF1 (A). Lipid assignments based on tandem mass spectrometry (MS/MS) identified changes in the phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ), or the individual fatty acids and their respective position (sn 1 /sn 2 ).

    Journal: Analytical Chemistry

    Article Title: Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

    doi: 10.1021/acs.analchem.7b02265

    Figure Lengend Snippet: Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in negative ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in DF1 (A). Lipid assignments based on tandem mass spectrometry (MS/MS) identified changes in the phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ), or the individual fatty acids and their respective position (sn 1 /sn 2 ).

    Article Snippet: Lipid Identification Lipid identification was performed with tandem mass spectrometry (MS/MS) in both positive and negative ion modes using a Synapt G2Si HDMS Q-ToF (Waters) after completing MALDI-MSI experiments on adjacent sections.

    Techniques: Cell Culture, Mass Spectrometry

    Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in positive ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in the first discriminant function (DF1) (A). Lipid assignments based on tandem mass spectrometry (MS/MS) identified changes in the phosphatidylcholine (PC) and sphingomyelin (SM) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ) or the individual fatty acids and their respective position (sn 1 /sn 2 ).

    Journal: Analytical Chemistry

    Article Title: Oxygen-Dependent Lipid Profiles of Three-Dimensional Cultured Human Chondrocytes Revealed by MALDI-MSI

    doi: 10.1021/acs.analchem.7b02265

    Figure Lengend Snippet: Chondrocyte cell pellets cultured in hypoxia (2.5% O 2 ) and normoxia (20% O 2 ) were analyzed with MALDI-MSI in positive ion mode. The average spectra of all donors revealed a clear spectral difference between the oxygen levels in the first discriminant function (DF1) (A). Lipid assignments based on tandem mass spectrometry (MS/MS) identified changes in the phosphatidylcholine (PC) and sphingomyelin (SM) content of the cell pellets (B). The MS/MS spectra defined the fatty acid composition and position as the total of the two chains (sn x + y ), the individual fatty acids (sn x _sn y ) or the individual fatty acids and their respective position (sn 1 /sn 2 ).

    Article Snippet: Lipid Identification Lipid identification was performed with tandem mass spectrometry (MS/MS) in both positive and negative ion modes using a Synapt G2Si HDMS Q-ToF (Waters) after completing MALDI-MSI experiments on adjacent sections.

    Techniques: Cell Culture, Mass Spectrometry