Journal: bioRxiv

Article Title: TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth

doi: 10.1101/2022.02.20.481212

Figure Lengend Snippet: (A) BRF1 and TIF-1A were co-localized in the nucleoli of HeLa cells. IF staining was performed using the BRF1 and TIF-1A antibodies. The scale bar in each image represents 2.5 μm. ( B ) BRF1 and RPA40 were co-localized in the nucleoli of HeLa cells. IF staining was performed using BRF1 antibody and RPA40 antibody. The scale bar in each image represents 2.5 μm. ( C ) The co-localization analysis between BRF1 and TIF-1A using the nucleolar particles purified from HeLa cells. The scale bar in each image represents 2.5 μm. ( D ) The co-localization analysis between BRF1 and RPA43 using the nucleoli particles purified from HeLa cells. The scale bar in each image represents 5 μm. ( E ) Components of the Pol I transcription machinery could be precipitated by the BRF1 antibody. HeLa nuclear extract was prepared and used for BRF1 IP assays. BRF1-binding proteins in the IP samples were detected by Western blot using the antibodies as indicated in the panel. ( F , G ) BRF1 could be precipitated by the antibodies against Pol I-related proteins. IP assays were performed with the antibodies against TBP, TIF-1A, TAF1A, UBF, and RPA43, respectively. BRF1 ( F ) and proteins as indicated ( G ) were detected by Western blot. Figure 6-figure supplement 1. The co-localization analysis between BRF1 and Pol III transcription factors in HeLa cells. Figure 6-figure supplement 2. BRF1 binds to TFIIIC subunit GTF3C2 and SL1 subunit TAF1B. Figure 6-source data, Figure 6-figure supplement 2-source data

Article Snippet: IP assays were performed using normal IgG, BRF1 antibody and the antibodies against UBF (ab244287, Abcam), TIF-1A (ab251933, Abcam), TBP (ab28175, abcam), TAF1A (SC-393600, Santa Cruz Biotech), GTF3C2 (SC-81406, Santa Cruz Biotech), TAF1B (CSB-PA684476ESR1HU, CUSABio) and RPA43 (Ab99305, Abcam), respectively.

Techniques: Staining, Purification, Binding Assay, Western Blot

Journal: bioRxiv

Article Title: TFIIB-related factor 1 is a nucleolar protein that promotes RNA polymerase I-directed transcription and tumour cell growth

doi: 10.1101/2022.02.20.481212

Figure Lengend Snippet: (A) BRF1 IP results showing BRF1 association with GTF3C2 and TAF1B. IP assays were performed using BRF1 antibody and HeLa nuclear extract. BRF1-bound protein was detected by Western blot using the antibodies as indicated. ( B ) GTF3C2 IP result showing GT3C2 association with BRF1. IP assays were performed using GTF3C2 antibody and HeLa nuclear extract. GTF3C2-bound protein was detected by Western blot using the antibodies as indicated. ( C ) TAF1B IP result showing TAF1B association with BRF1. IP assays were performed using TAF1B antibody and HeLa nuclear extract. TAF1B-bound protein was detected by Western blot using the antibodies as indicated.

Article Snippet: IP assays were performed using normal IgG, BRF1 antibody and the antibodies against UBF (ab244287, Abcam), TIF-1A (ab251933, Abcam), TBP (ab28175, abcam), TAF1A (SC-393600, Santa Cruz Biotech), GTF3C2 (SC-81406, Santa Cruz Biotech), TAF1B (CSB-PA684476ESR1HU, CUSABio) and RPA43 (Ab99305, Abcam), respectively.

Techniques: Western Blot

Journal: Nature Communications

Article Title: Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity

doi: 10.1038/ncomms14364

Figure Lengend Snippet: Markers achieving genome-wide significance in GWAS of 3,379 NSCLP cases and 8,593 controls of Chinese Han and prioritized genes in each significant SNP.

Article Snippet: The rabbit polyclonal antibodies to Fam49a (LS-C167900, LSbio; 1:100 dilution), Rad54b (orb100108, Biorbyt; 1:200 dilution), Rps26 (14909-1-AP, Proteintech; 1:800 dilution), Taf1b (12818-1-AP, Proteintech; 1:600 dilution) and Thap2 (orb186252, Biorbyt; 1:200 dilution) were incubated with the sections at 4 °C overnight, respectively, and were then detected with the Rabbit SP kit (SP9001, Zhongshan Golden Bridge Biotech).

Techniques: Genome Wide

Journal: Nature Communications

Article Title: Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity

doi: 10.1038/ncomms14364

Figure Lengend Snippet: Markers achieving genome-wide significance in GWAS of 3,379 NSCLP cases and 8,593 controls of Chinese Han and prioritized genes in each significant SNP.

Article Snippet: The rabbit polyclonal antibodies to Fam49a (LS-C167900, LSbio; 1:100 dilution), Rad54b (orb100108, Biorbyt; 1:200 dilution), Rps26 (14909-1-AP, Proteintech; 1:800 dilution), Taf1b (12818-1-AP, Proteintech; 1:600 dilution) and Thap2 (orb186252, Biorbyt; 1:200 dilution) were incubated with the sections at 4 °C overnight, respectively, and were then detected with the Rabbit SP kit (SP9001, Zhongshan Golden Bridge Biotech).

Techniques: Genome Wide