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nvp tae 226  (MedChemExpress)
94
MedChemExpress nvp tae 226
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tae  (Thermo Fisher)
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Tae, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1x biorad 9 hercules ca cat 1610743  (Bio-Rad)
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buffer solution  (Thermo Fisher)
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Thermo Fisher buffer solution
Buffer Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tae buffer  (Thermo Fisher)
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Thermo Fisher tae buffer
Tae Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tae  (MedChemExpress)
93
MedChemExpress tae
Figure 5. SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V3 KD (kinase-dead) for 24 hours. They were then treated with either DMSO alone or 0.5 mmol/L nocodazole for 16 hours before being analysed by flow cytometry. The top row indicates how cells were gated for expression of YFP using green channel forward scatter with the gate set above the upper threshold of fluorescence in the mock-transfected cells. The middle and bottom rows indicate DNA content following treatment with DMSO or nocodazole, respectively, with the profile shown for the ungated mock-transfected cells but the gated cells for those transfected with the different plasmids. B, The percentage of cells in G2–M were determined following treatment described in A. , P < 0.05 compared with DMSO by unpaired T-test. C, H2228 cells were treated with DMSO alone or 0.149 <t>mmol/L</t> <t>TAE-684</t> and analysed by time-lapse imaging; images captured at the times indicated (minutes) are shown. D, Quantification of mitotic duration for cells treated as in C. , P < 0.001 compared with DMSO by unpaired T test. E, H2228 cells were either mock-depleted or depleted of EML4-ALK using siALK.1 or siALK.2 and analysed as in C. F, Quantification of mitotic duration for cells treated as in E. , P < 0.01 and , P < 0.001 compared with siMock by one-way ANOVA. Data are means þSD for three independent experiments (indicated in different colours in D and F). The three different shapes and colors in the dot plots in D and F indicate data from three independent experiments, with large shapes referring to means and small shapes the individual data points. Scale bars, 20 mm.
Tae, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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50x tris acetic acid edta tae  (Bio-Rad)
93
Bio-Rad 50x tris acetic acid edta tae
Figure 5. SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V3 KD (kinase-dead) for 24 hours. They were then treated with either DMSO alone or 0.5 mmol/L nocodazole for 16 hours before being analysed by flow cytometry. The top row indicates how cells were gated for expression of YFP using green channel forward scatter with the gate set above the upper threshold of fluorescence in the mock-transfected cells. The middle and bottom rows indicate DNA content following treatment with DMSO or nocodazole, respectively, with the profile shown for the ungated mock-transfected cells but the gated cells for those transfected with the different plasmids. B, The percentage of cells in G2–M were determined following treatment described in A. , P < 0.05 compared with DMSO by unpaired T-test. C, H2228 cells were treated with DMSO alone or 0.149 <t>mmol/L</t> <t>TAE-684</t> and analysed by time-lapse imaging; images captured at the times indicated (minutes) are shown. D, Quantification of mitotic duration for cells treated as in C. , P < 0.001 compared with DMSO by unpaired T test. E, H2228 cells were either mock-depleted or depleted of EML4-ALK using siALK.1 or siALK.2 and analysed as in C. F, Quantification of mitotic duration for cells treated as in E. , P < 0.01 and , P < 0.001 compared with siMock by one-way ANOVA. Data are means þSD for three independent experiments (indicated in different colours in D and F). The three different shapes and colors in the dot plots in D and F indicate data from three independent experiments, with large shapes referring to means and small shapes the individual data points. Scale bars, 20 mm.
50x Tris Acetic Acid Edta Tae, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tae tris acetate edta buffer  (Thermo Fisher)
96
Thermo Fisher tae tris acetate edta buffer
Figure 5. SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V3 KD (kinase-dead) for 24 hours. They were then treated with either DMSO alone or 0.5 mmol/L nocodazole for 16 hours before being analysed by flow cytometry. The top row indicates how cells were gated for expression of YFP using green channel forward scatter with the gate set above the upper threshold of fluorescence in the mock-transfected cells. The middle and bottom rows indicate DNA content following treatment with DMSO or nocodazole, respectively, with the profile shown for the ungated mock-transfected cells but the gated cells for those transfected with the different plasmids. B, The percentage of cells in G2–M were determined following treatment described in A. , P < 0.05 compared with DMSO by unpaired T-test. C, H2228 cells were treated with DMSO alone or 0.149 <t>mmol/L</t> <t>TAE-684</t> and analysed by time-lapse imaging; images captured at the times indicated (minutes) are shown. D, Quantification of mitotic duration for cells treated as in C. , P < 0.001 compared with DMSO by unpaired T test. E, H2228 cells were either mock-depleted or depleted of EML4-ALK using siALK.1 or siALK.2 and analysed as in C. F, Quantification of mitotic duration for cells treated as in E. , P < 0.01 and , P < 0.001 compared with siMock by one-way ANOVA. Data are means þSD for three independent experiments (indicated in different colours in D and F). The three different shapes and colors in the dot plots in D and F indicate data from three independent experiments, with large shapes referring to means and small shapes the individual data points. Scale bars, 20 mm.
Tae Tris Acetate Edta Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris acetate edta tae buffer  (Genesee Scientific)
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Genesee Scientific tris acetate edta tae buffer
Figure 5. SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V3 KD (kinase-dead) for 24 hours. They were then treated with either DMSO alone or 0.5 mmol/L nocodazole for 16 hours before being analysed by flow cytometry. The top row indicates how cells were gated for expression of YFP using green channel forward scatter with the gate set above the upper threshold of fluorescence in the mock-transfected cells. The middle and bottom rows indicate DNA content following treatment with DMSO or nocodazole, respectively, with the profile shown for the ungated mock-transfected cells but the gated cells for those transfected with the different plasmids. B, The percentage of cells in G2–M were determined following treatment described in A. , P < 0.05 compared with DMSO by unpaired T-test. C, H2228 cells were treated with DMSO alone or 0.149 <t>mmol/L</t> <t>TAE-684</t> and analysed by time-lapse imaging; images captured at the times indicated (minutes) are shown. D, Quantification of mitotic duration for cells treated as in C. , P < 0.001 compared with DMSO by unpaired T test. E, H2228 cells were either mock-depleted or depleted of EML4-ALK using siALK.1 or siALK.2 and analysed as in C. F, Quantification of mitotic duration for cells treated as in E. , P < 0.01 and , P < 0.001 compared with siMock by one-way ANOVA. Data are means þSD for three independent experiments (indicated in different colours in D and F). The three different shapes and colors in the dot plots in D and F indicate data from three independent experiments, with large shapes referring to means and small shapes the individual data points. Scale bars, 20 mm.
Tris Acetate Edta Tae Buffer, supplied by Genesee Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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readyagarose 96 plus gel  (Bio-Rad)
86
Bio-Rad readyagarose 96 plus gel
Figure 5. SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V3 KD (kinase-dead) for 24 hours. They were then treated with either DMSO alone or 0.5 mmol/L nocodazole for 16 hours before being analysed by flow cytometry. The top row indicates how cells were gated for expression of YFP using green channel forward scatter with the gate set above the upper threshold of fluorescence in the mock-transfected cells. The middle and bottom rows indicate DNA content following treatment with DMSO or nocodazole, respectively, with the profile shown for the ungated mock-transfected cells but the gated cells for those transfected with the different plasmids. B, The percentage of cells in G2–M were determined following treatment described in A. , P < 0.05 compared with DMSO by unpaired T-test. C, H2228 cells were treated with DMSO alone or 0.149 <t>mmol/L</t> <t>TAE-684</t> and analysed by time-lapse imaging; images captured at the times indicated (minutes) are shown. D, Quantification of mitotic duration for cells treated as in C. , P < 0.001 compared with DMSO by unpaired T test. E, H2228 cells were either mock-depleted or depleted of EML4-ALK using siALK.1 or siALK.2 and analysed as in C. F, Quantification of mitotic duration for cells treated as in E. , P < 0.01 and , P < 0.001 compared with siMock by one-way ANOVA. Data are means þSD for three independent experiments (indicated in different colours in D and F). The three different shapes and colors in the dot plots in D and F indicate data from three independent experiments, with large shapes referring to means and small shapes the individual data points. Scale bars, 20 mm.
Readyagarose 96 Plus Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V3 KD (kinase-dead) for 24 hours. They were then treated with either DMSO alone or 0.5 mmol/L nocodazole for 16 hours before being analysed by flow cytometry. The top row indicates how cells were gated for expression of YFP using green channel forward scatter with the gate set above the upper threshold of fluorescence in the mock-transfected cells. The middle and bottom rows indicate DNA content following treatment with DMSO or nocodazole, respectively, with the profile shown for the ungated mock-transfected cells but the gated cells for those transfected with the different plasmids. B, The percentage of cells in G2–M were determined following treatment described in A. , P < 0.05 compared with DMSO by unpaired T-test. C, H2228 cells were treated with DMSO alone or 0.149 mmol/L TAE-684 and analysed by time-lapse imaging; images captured at the times indicated (minutes) are shown. D, Quantification of mitotic duration for cells treated as in C. , P < 0.001 compared with DMSO by unpaired T test. E, H2228 cells were either mock-depleted or depleted of EML4-ALK using siALK.1 or siALK.2 and analysed as in C. F, Quantification of mitotic duration for cells treated as in E. , P < 0.01 and , P < 0.001 compared with siMock by one-way ANOVA. Data are means þSD for three independent experiments (indicated in different colours in D and F). The three different shapes and colors in the dot plots in D and F indicate data from three independent experiments, with large shapes referring to means and small shapes the individual data points. Scale bars, 20 mm.

Journal: Molecular cancer research : MCR

Article Title: EML4-ALK Variant 3 Promotes Mitotic Errors and Spindle Assembly Checkpoint Deficiency Leading to Increased Microtubule Poison Sensitivity.

doi: 10.1158/1541-7786.MCR-21-1010

Figure Lengend Snippet: Figure 5. SAC deficiencies in cells expressing EML4-ALK V3 are dependent on ALK activity. A, Beas-2B parental cells were either mock transfected or transfected with plasmids encoding YFP alone, YFP-EML4-ALK V3 WT (wild-type) or YFP-EML4-ALK V3 KD (kinase-dead) for 24 hours. They were then treated with either DMSO alone or 0.5 mmol/L nocodazole for 16 hours before being analysed by flow cytometry. The top row indicates how cells were gated for expression of YFP using green channel forward scatter with the gate set above the upper threshold of fluorescence in the mock-transfected cells. The middle and bottom rows indicate DNA content following treatment with DMSO or nocodazole, respectively, with the profile shown for the ungated mock-transfected cells but the gated cells for those transfected with the different plasmids. B, The percentage of cells in G2–M were determined following treatment described in A. , P < 0.05 compared with DMSO by unpaired T-test. C, H2228 cells were treated with DMSO alone or 0.149 mmol/L TAE-684 and analysed by time-lapse imaging; images captured at the times indicated (minutes) are shown. D, Quantification of mitotic duration for cells treated as in C. , P < 0.001 compared with DMSO by unpaired T test. E, H2228 cells were either mock-depleted or depleted of EML4-ALK using siALK.1 or siALK.2 and analysed as in C. F, Quantification of mitotic duration for cells treated as in E. , P < 0.01 and , P < 0.001 compared with siMock by one-way ANOVA. Data are means þSD for three independent experiments (indicated in different colours in D and F). The three different shapes and colors in the dot plots in D and F indicate data from three independent experiments, with large shapes referring to means and small shapes the individual data points. Scale bars, 20 mm.

Article Snippet: TAE-684 was obtained from MedChemExpress and reconstituted in DMSO. siRNA depletion Cells were seeded in a 6-well dish such that they reached 30% to 40% confluency on the day of transfection in OptiMEM reduced serum media (Invitrogen) with 10% FBS and no antibiotics.

Techniques: Expressing, Activity Assay, Transfection, Cytometry, Imaging