Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Th2 cells lacking T-bet suppress naive and memory T cell responses via IL-10

doi: 10.1073/pnas.2002787118

Figure Lengend Snippet: Tbx21−/− Th2 cells impair CD8+ T cell activation via IL-10 in vitro. (A) Schematic experimental layout to investigate the effect of LCMV-infected DCs previously conditioned by Tbx21−/− Th2 or Il10−/−Tbx21−/− Th2 cells on p14 cell activation in vitro. After 2 d of LCMV infection, CD4+ LCMV-specific T cells were depleted from T cell–DC cocultures. These conditioned DCs were subsequently cocultured with p14 cells in the presence of GP33 for 3 additional days. (B) Expression of surface markers CD69, KLRG-1, CD44, CD25, CD62L, and CD127 as well as (C) IFN-γ and CD107a expression (geometric mean of fluorescence intensity) by p14 cells after activation in vitro with LCMV-infected DCs that were previously conditioned with Tbx21−/− Th2 cells or Il10−/−Tbx21−/− Th2 cells. All experiments were performed at least twice, and each experimental group included n ≥ 3. Data are pooled and expressed as mean ± SEM. Asterisks indicate statistically significant differences as analyzed by one-way ANOVA with Bonferroni’s post test (*P < 0.05).

Article Snippet: For CD4 + T cell transfers, SMARTA1 LCMV-specific T cells of Tbx21 −/− , Il10 −/− Tbx21 −/− , and WT Thy1.1 + tg mice of the respective knockout backgrounds (6 to 8 wk old) were purified by staining with biotinylated antibodies against CD8 (53-6.7), CD11c (N418), CD11b (M1/70), CD19 (1D3), NK1.1 (PK136), Gr-1 (RB6-8C5), and CD25 (7D4), followed by anti-biotin microbeads and magnetic depletion (Miltenyi Biotec).

Techniques: Activation Assay, In Vitro, Infection, Expressing, Fluorescence

Journal: iScience

Article Title: DUSP6 deletion protects mice and reduces disease severity in autoimmune arthritis

doi: 10.1016/j.isci.2024.110158

Figure Lengend Snippet:

Article Snippet: anti-CD25-APC (clone: 4E3) , Miltenyi Biotec, Germany , Cat# 130-113-846.

Techniques: Transgenic Assay, Knock-Out