Journal: Science (New York, N.Y.)

Article Title: Loci associated with skin pigmentation identified in African populations

doi: 10.1126/science.aan8433

Figure Lengend Snippet: Immortalized melan-lnk4a melanocytes expressing non-target (sh NT) shRNA or either of two shRNA plasmid clones (#1 and 2) targeting Mfsd12 were analyzed for Mfsd12 mRNA content by (A) qRT-PCR, (B) melanin content by spectrophotometry, or (C) percent of cell area containing melanin by bright field microscopy; (D) shows quantification. Data in (A) to (C) represent mean ± s.e.m., normalized to sh NT samples, from three separate experiments; in (C), n(sh NT) = 97 cells, n (shMfsd12 #1) = 68 cells, n (shMfsd12 #2) = 71 cells. Bar in (C), 10 μm. (E to G) Melan-ink4a melanocytes transiently expressing MFSD12-HA (E) or not transfected (F) and (G) were fixed, immunolabeled for HA (E) and for LAMP2 to mark lysosomes E) and (G) or for TYRP1 to mark melanosomes (G), and analyzed by immunofluorescence and bright field microscopy. Bright field (melanin) images show pigmented melanosomes (pseudocolored red in the merged images). Insets, 4x magnification of boxed regions. Arrows, MFSD12-containing structures that overlap LAMP2 (E) or TYRP1-containing structures that overlap melanosomes; arrowheads, structures that do not overlap. Bars, 10 μm. (H) Quantification of overlap for structures labeled by MFSD12, TYRP1, LAMP2 and pigment. Data represent mean ± s.e.m. from three independent experiments, n = 17 cells (MFSD12 overlap with LAMP2 and melanin), 33 cells (TYRP1 overlap with melanin), or 23 cells (LAMP2 and melanin).

Article Snippet: To analyze MFSD12-HA localization, melan-Ink4a melanocytes were transiently transfected with MFSD12-HA expression plasmids and analyzed 48 hours later by bright field and immunofluorescence microscopy as described ( 100 ) using the TA99 monoclonal antibody to TYRP1 (American Type Culture Collection) to detect melanosomes, rabbit anti-LAMP2A (Abcam) to detect lysosomes, and rat anti-HA (Roche) to detect the transgene.

Techniques: Expressing, shRNA, Plasmid Preparation, Clone Assay, Quantitative RT-PCR, Spectrophotometry, Microscopy, Transfection, Immunolabeling, Immunofluorescence, Labeling

Journal: eLife

Article Title: Chromosomal instability induced in cancer can enhance macrophage-initiated immune responses that include anti-tumor IgG

doi: 10.7554/eLife.88054

Figure Lengend Snippet:

Article Snippet: Antibody , InVivoMab mouse anti-human/mouse Tryp1 (clone TA99) , BioXCell , Cat# BE0151; RRID: AB_10949462 , (1:1000) Opsonization (10–20 μg/mL) In vivo (250 μg/100 μL per dose).

Techniques: Derivative Assay, Adoptive Transfer Assay, Centrifugation, In Vivo, Control, Blocking Assay, Recombinant, Sequencing, Multiplex Assay, Gene Expression, Software, Red Blood Cell Lysis

Journal: Molecules

Article Title: Polymer Nanoparticles with 2-HP-β-Cyclodextrin for Enhanced Retention of Uptake into HCE-T Cells

doi: 10.3390/molecules29030658

Figure Lengend Snippet: The cumulative release rate of triamcinolone acetonide in artificial aqueous humor (mean ± SD, n = 6) (●: TA aqueous suspension; ■: (2-HP-β-CD)/PLGA-loaded nanoparticles).

Article Snippet: Triamcinolone acetonide (TA, 99.9%) was obtained from Hehui Pharmaceutical Group Co., Ltd. (Tianjin, China).

Techniques: Suspension

Journal: Molecules

Article Title: Polymer Nanoparticles with 2-HP-β-Cyclodextrin for Enhanced Retention of Uptake into HCE-T Cells

doi: 10.3390/molecules29030658

Figure Lengend Snippet: The release models of triamcinolone acetonide in vitro.

Article Snippet: Triamcinolone acetonide (TA, 99.9%) was obtained from Hehui Pharmaceutical Group Co., Ltd. (Tianjin, China).

Techniques: Suspension

Journal: Molecules

Article Title: Polymer Nanoparticles with 2-HP-β-Cyclodextrin for Enhanced Retention of Uptake into HCE-T Cells

doi: 10.3390/molecules29030658

Figure Lengend Snippet: The permeability of triamcinolone acetonide across excised rabbit corneas (mean ± SD, n = 3). (●: TA aqueous suspension; ■: (2-HP-β-CD)/PLGA-loaded nanoparticles).

Article Snippet: Triamcinolone acetonide (TA, 99.9%) was obtained from Hehui Pharmaceutical Group Co., Ltd. (Tianjin, China).

Techniques: Permeability, Suspension

Journal: Molecules

Article Title: Polymer Nanoparticles with 2-HP-β-Cyclodextrin for Enhanced Retention of Uptake into HCE-T Cells

doi: 10.3390/molecules29030658

Figure Lengend Snippet: Concentration–time curves of triamcinolone acetonide in aqueous humor (mean ± SD, n = 3). (●: TA aqueous suspension; ■: (2-HP-β-CD)/PLGA-loaded nanoparticles).

Article Snippet: Triamcinolone acetonide (TA, 99.9%) was obtained from Hehui Pharmaceutical Group Co., Ltd. (Tianjin, China).

Techniques: Concentration Assay, Suspension