t4 ligase Promega Search Results


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  • 99
    New England Biolabs t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6737 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/New England Biolabs
    Average 99 stars, based on 6737 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    96
    Promega t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 1682 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase/product/Promega
    Average 96 stars, based on 1682 article reviews
    Price from $9.99 to $1999.99
    t4 ligase - by Bioz Stars, 2020-01
    96/100 stars
      Buy from Supplier

    89
    Promega t4 ligase buffer
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    T4 Ligase Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 ligase buffer/product/Promega
    Average 89 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    t4 ligase buffer - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    86
    Promega hc t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Hc T4 Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hc t4 ligase/product/Promega
    Average 86 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    hc t4 ligase - by Bioz Stars, 2020-01
    86/100 stars
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    78
    Promega dna t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    Dna T4 Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna t4 ligase/product/Promega
    Average 78 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dna t4 ligase - by Bioz Stars, 2020-01
    78/100 stars
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    76
    Promega high concentration t4 ligase
    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of <t>T4</t> ligase
    High Concentration T4 Ligase, supplied by Promega, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high concentration t4 ligase/product/Promega
    Average 76 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    high concentration t4 ligase - by Bioz Stars, 2020-01
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    Image Search Results


    PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of T4 ligase

    Journal: Analytical and bioanalytical chemistry

    Article Title: Heterobifunctional modification of DNA for conjugation to solid surfaces

    doi: 10.1007/s00216-010-3733-5

    Figure Lengend Snippet: PAGE gel demonstrating the generation of a 150-mer fragment after successful adapter ligation. Lanes A 100 bp without ligase, B 100-mer with 1,000 units of T4 ligase, C 100-mer and 25 molar excess adapters with T4 ligase, D dA-tailed 100-mer with T4 ligase, E adapter without ligase, F adapter with T4 ligase, G dA-tailed 100-mer and 25 M excess adapters with 1,000 units of T4 ligase

    Article Snippet: We observed that, under conditions suggested by the suppliers of the T4 ligase (NEB and Promega), the predominant product was persistently a single-addition product (~100% 125-mer versus ~0% of 150-mer DNA), whereas the yield of conversion was relatively low ( > 70% of original DNA remained unmodified in spite of the 25-fold molar excess of adaptors).

    Techniques: Polyacrylamide Gel Electrophoresis, Ligation

    Analysis of PAGE data. a Image intensity profiles are averaged over 20–40 pixels in a given lane and then fitted to multiple Gaussian peaks (using IGOR Pro, Wavemetrics) representing 100-mer-, 125-mer-, and 150-mer-long DNA fragments. Peak areas (normalized by the total fragment length) are used as a quantitative measure of the molar amount of given oligomers. b Time course of changes in the molar fraction of the dA-tailed unmodified DNA (100-mer) and DNA ligated at one end (125-mer) or both ends (150-mer). Six picomoles of 100-mer DNA with 25 molar excess adapters and 400 units of T4 ligase in a total volume of 50 μL were incubated at 16 °C. c Dependence of the conversion of the dA-tailed DNA into end-modified DNA on the concentration of the enzyme for 6-h-long reactions. Six picomoles of 100-mer DNA and 25 molar excess of adapters in a total volume of 10 μL were incubated at 16 °C. Curves in b and c are fits to exponential or double-exponential functions and serve as guides to the eye

    Journal: Analytical and bioanalytical chemistry

    Article Title: Heterobifunctional modification of DNA for conjugation to solid surfaces

    doi: 10.1007/s00216-010-3733-5

    Figure Lengend Snippet: Analysis of PAGE data. a Image intensity profiles are averaged over 20–40 pixels in a given lane and then fitted to multiple Gaussian peaks (using IGOR Pro, Wavemetrics) representing 100-mer-, 125-mer-, and 150-mer-long DNA fragments. Peak areas (normalized by the total fragment length) are used as a quantitative measure of the molar amount of given oligomers. b Time course of changes in the molar fraction of the dA-tailed unmodified DNA (100-mer) and DNA ligated at one end (125-mer) or both ends (150-mer). Six picomoles of 100-mer DNA with 25 molar excess adapters and 400 units of T4 ligase in a total volume of 50 μL were incubated at 16 °C. c Dependence of the conversion of the dA-tailed DNA into end-modified DNA on the concentration of the enzyme for 6-h-long reactions. Six picomoles of 100-mer DNA and 25 molar excess of adapters in a total volume of 10 μL were incubated at 16 °C. Curves in b and c are fits to exponential or double-exponential functions and serve as guides to the eye

    Article Snippet: We observed that, under conditions suggested by the suppliers of the T4 ligase (NEB and Promega), the predominant product was persistently a single-addition product (~100% 125-mer versus ~0% of 150-mer DNA), whereas the yield of conversion was relatively low ( > 70% of original DNA remained unmodified in spite of the 25-fold molar excess of adaptors).

    Techniques: Polyacrylamide Gel Electrophoresis, Incubation, Modification, Concentration Assay