t25 flasks Search Results


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  • 99
    Thermo Fisher t25 culture flasks
    DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in <t>T25</t> culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope
    T25 Culture Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 culture flasks/product/Thermo Fisher
    Average 99 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    t25 culture flasks - by Bioz Stars, 2020-04
    99/100 stars
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    91
    Corning Life Sciences t 25 flasks
    The tissue specimens, approximately 1×1 mm in size, placed in a <t>T-25</t> flask
    T 25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t 25 flasks/product/Corning Life Sciences
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    t 25 flasks - by Bioz Stars, 2020-04
    91/100 stars
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    94
    Becton Dickinson t25 flasks
    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in <t>T25</t> flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm
    T25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Becton Dickinson
    Average 94 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
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    95
    Sarstedt t25 flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Sarstedt
    Average 95 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    95/100 stars
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    93
    Greiner Bio t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 93/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Greiner Bio
    Average 93 stars, based on 124 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    93/100 stars
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    93
    Avantor t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Avantor
    Average 93 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    93/100 stars
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    92
    Fisher Scientific t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Fisher Scientific
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    92/100 stars
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    95
    Becton Dickinson t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Becton Dickinson
    Average 95 stars, based on 110 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    95/100 stars
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    92
    PAA Laboratories t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/PAA Laboratories
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    92/100 stars
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    93
    Fisher Scientific t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Fisher Scientific
    Average 93 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    93/100 stars
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    86
    Iwaki America t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Iwaki America, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Iwaki America
    Average 86 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    86/100 stars
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    95
    Corning Life Sciences t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Corning Life Sciences
    Average 95 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    95/100 stars
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    94
    SPL Life Sciences t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/SPL Life Sciences
    Average 94 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    88
    Orange Scientific t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Orange Scientific, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Orange Scientific
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    88/100 stars
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    92
    Sarstedt t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Sarstedt, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Sarstedt
    Average 92 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    94
    SPL Life Sciences t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by SPL Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/SPL Life Sciences
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    94/100 stars
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    91
    Orange Scientific t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Orange Scientific, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Orange Scientific
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    93
    Millipore t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Millipore
    Average 93 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    94
    Bio Basic Canada t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Avantor, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Boster Bio t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson vented t 25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    Vented T 25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Fisher Scientific ps t 25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    Ps T 25 Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson t25 cm2 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Cm2 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Corning Life Sciences primaria t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    Primaria T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Corning Life Sciences t25 t75 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 T75 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ABM Inc pricoat t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    Pricoat T25 Flasks, supplied by ABM Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Greiner Bio t25 vented flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Vented Flasks, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson t25 falcon flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Falcon Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Corning Life Sciences t25 cm2 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Cm2 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson t25 primaria flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Primaria Flask, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Corning Life Sciences t25 falcon flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Falcon Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in T25 culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope

    Journal: Analytical and Bioanalytical Chemistry

    Article Title: Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy

    doi: 10.1007/s00216-018-0987-9

    Figure Lengend Snippet: DBTRG cells; control versus contaminated cells after 24 h mycoplasma infection in T25 culture flasks (supernatant Myc+ corresponds to dilution 1:100 supernatant Myc+++) shown using an inverted microscope

    Article Snippet: Cell preparation DBTRG cells (a human brain glioblastoma cell line, ATCC number CRL-2020) were seeded in T25 culture flasks at a density of 1 × 106 cells/ml and cultured in RPMI 1640 medium supplemented with 10% FBS, 2 mM GlutaMax, (all from Gibco, Invitrogen).

    Techniques: Infection, Inverted Microscopy

    The tissue specimens, approximately 1×1 mm in size, placed in a T-25 flask

    Journal: Balkan medical journal

    Article Title: Ex Vivo Produced Oral Mucosa Equivalent by Using the Direct Explant Cell Culture Technique

    doi: 10.5152/balkanmedj.2012.011

    Figure Lengend Snippet: The tissue specimens, approximately 1×1 mm in size, placed in a T-25 flask

    Article Snippet: Disaggregated cells were collected, counted, centrifuged, resuspended and replated into new T-25 flasks (Corning, New York, USA) at a density of 2.0×104 cells/cm2 ( ).

    Techniques:

    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Journal: Stem Cell Research & Therapy

    Article Title: Full-length amelogenin influences the differentiation of human dental pulp stem cells

    doi: 10.1186/s13287-015-0269-9

    Figure Lengend Snippet: Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Article Snippet: The cells were seeded in T25 flasks (BD Falcon, San Jose, CA, USA), at a density of 2*104 cells/cm2 and cultured in a humidified atmosphere containing 5 % CO2 at 37 °C, with the medium changed twice a week.

    Techniques: Light Microscopy, Cell Culture, Modification

    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Journal: PLoS ONE

    Article Title: Cell colony counter called CoCoNut

    doi: 10.1371/journal.pone.0205823

    Figure Lengend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Article Snippet: The count for viable cells was used for plaiting cells in either T25 flasks (25 cm2 cell culture area; Sarstedt, Skanderborg, Denmark) with ventilated caps containing 6 ml complete medium or in 35 mm cell culture dishes (Sigma-Aldrich) with 3 ml complete medium, respectively.

    Techniques: Cell Culture

    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into T25, incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).

    Journal: PLoS ONE

    Article Title: Indication of Horizontal DNA Gene Transfer by Extracellular Vesicles

    doi: 10.1371/journal.pone.0163665

    Figure Lengend Snippet: Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into T25, incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).

    Article Snippet: EV-transfer to recipient cells Recipient human BM-hMSC (2.5-5x104 ) unrelated to hMSC used for A .t .-transduction were seeded into T25-flasks (Greiner).

    Techniques: Fluorescence, Passaging, Incubation, Derivative Assay, SYBR Green Assay, Real-time Polymerase Chain Reaction, Positive Control, Polymerase Chain Reaction, Negative Control

    Alteration in protein levels and phosphorylation on short term AZD8055 treatment Cells at CPD 36 or CPD 73 were seeded into T25 flasks and grown in medium containing AZD8055 or DMSO for one week. After harvesting, cells were pelleted and lysed in RIPA with protease and phosphatase inhibitors, separated on SDS-PAGE then immunoblotted for the indicated proteins. ( A ) Components of the mTOR signaling pathway, ( B ) Cell cycle proteins. The panels on the right show longer exposure images of the same panels on the left, to highlight bands that are faint in the shorter exposure images.

    Journal: Aging (Albany NY)

    Article Title: Reversal of phenotypes of cellular senescence by pan-mTOR inhibition

    doi:

    Figure Lengend Snippet: Alteration in protein levels and phosphorylation on short term AZD8055 treatment Cells at CPD 36 or CPD 73 were seeded into T25 flasks and grown in medium containing AZD8055 or DMSO for one week. After harvesting, cells were pelleted and lysed in RIPA with protease and phosphatase inhibitors, separated on SDS-PAGE then immunoblotted for the indicated proteins. ( A ) Components of the mTOR signaling pathway, ( B ) Cell cycle proteins. The panels on the right show longer exposure images of the same panels on the left, to highlight bands that are faint in the shorter exposure images.

    Article Snippet: Cells were seeded at 2×105 per T25 flask (Greiner), or at 1×104 in 24 well plates (Greiner).

    Techniques: SDS Page