t25 flask Search Results


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  • 99
    Thermo Fisher t25 flasks
    T25 Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 988 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Thermo Fisher
    Average 99 stars, based on 988 article reviews
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    t25 flasks - by Bioz Stars, 2020-11
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    99
    Millipore t25 flasks
    T25 Flasks, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Millipore
    Average 99 stars, based on 334 article reviews
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    t25 flasks - by Bioz Stars, 2020-11
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    92
    Corning Life Sciences t25 flasks
    T25 Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Corning Life Sciences
    Average 92 stars, based on 275 article reviews
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    t25 flasks - by Bioz Stars, 2020-11
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    92
    Becton Dickinson t25 flasks
    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in <t>T25</t> flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm
    T25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Becton Dickinson
    Average 92 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-11
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    92
    Greiner Bio t25 flasks
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flasks, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 92/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Greiner Bio
    Average 92 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-11
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    94
    Millipore t25 flask
    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into <t>T25,</t> incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).
    T25 Flask, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flask/product/Millipore
    Average 94 stars, based on 116 article reviews
    Price from $9.99 to $1999.99
    t25 flask - by Bioz Stars, 2020-11
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    93
    Sarstedt t25 flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Flasks, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Sarstedt
    Average 93 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-11
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    89
    Corning Life Sciences t25 tissue culture flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Tissue Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 89/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 tissue culture flasks/product/Corning Life Sciences
    Average 89 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    t25 tissue culture flasks - by Bioz Stars, 2020-11
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    92
    Fisher Scientific t25 flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 flasks/product/Fisher Scientific
    Average 92 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    t25 flasks - by Bioz Stars, 2020-11
    92/100 stars
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    90
    Corning Life Sciences t25 culture flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 culture flasks/product/Corning Life Sciences
    Average 90 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    t25 culture flasks - by Bioz Stars, 2020-11
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    89
    Corning Life Sciences t25 cell culture flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T25 Cell Culture Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t25 cell culture flasks/product/Corning Life Sciences
    Average 89 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    t25 cell culture flasks - by Bioz Stars, 2020-11
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    88
    Becton Dickinson collagen coated t25 flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    Collagen Coated T25 Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/collagen coated t25 flasks/product/Becton Dickinson
    Average 88 stars, based on 11 article reviews
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    collagen coated t25 flasks - by Bioz Stars, 2020-11
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    99
    Thermo Fisher nunc easyflask cell culture flasks
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    Nunc Easyflask Cell Culture Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 130 article reviews
    Price from $9.99 to $1999.99
    nunc easyflask cell culture flasks - by Bioz Stars, 2020-11
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    98
    Millipore t 25 culture flask
    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. <t>T25</t> cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.
    T 25 Culture Flask, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t 25 culture flask/product/Millipore
    Average 98 stars, based on 19 article reviews
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    t 25 culture flask - by Bioz Stars, 2020-11
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    Image Search Results


    Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Journal: Stem Cell Research & Therapy

    Article Title: Full-length amelogenin influences the differentiation of human dental pulp stem cells

    doi: 10.1186/s13287-015-0269-9

    Figure Lengend Snippet: Representative light microscopy images of human dental pulp stem cells (hDPSCs). The cells were seeded in T25 flasks at a density of 2*10 4 cells/cm 2 and cultured in minimal essential medium, α-modification supplemented with 10 % fetal bovine serum and 1 % Penicillin/Streptomicin and monitored at day 21 (×10) ( a ); hDPSCs after 21 days of cultivation with a supplement of 10 ng/mL ( b ), 100 ng/mL ( c ) and 1000 ng/mL amelogenin ( d ). Scale bars 100 μm

    Article Snippet: The cells were seeded in T25 flasks (BD Falcon, San Jose, CA, USA), at a density of 2*104 cells/cm2 and cultured in a humidified atmosphere containing 5 % CO2 at 37 °C, with the medium changed twice a week.

    Techniques: Light Microscopy, Cell Culture, Modification

    Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into T25, incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).

    Journal: PLoS ONE

    Article Title: Indication of Horizontal DNA Gene Transfer by Extracellular Vesicles

    doi: 10.1371/journal.pone.0163665

    Figure Lengend Snippet: Detection of Venus-fluorescence and A . t .-sequences in recipient cells after passaging. (a) 2x10 5 hMSC were seeded into T25, incubated overnight to reach adherence (d0) and fed with EV derived from A . t .-hMSC cultures for 2 weeks. (b) Venus-positive cells were detected in 2 of 4 flasks (d14). One flask with 7 positive cells was passaged into 4xT25 flasks. (c) 7 days later (d21), one flask contained 10 Venus-positive cells. This culture was expanded again into 4xT25. (d) Venus-positive cells at d28 were evident in 2 flasks out of 4 with 13 cells in one flask and 1 cell in the second flask. Exemplarily, one positive MSC spot with corresponding phase-contrast for each time point is shown. Magnification x 100. (e, f) DNA of the flask with 13 Venus-positive cells was pretested in nested SYBR Green-based qPCR. Out of 10 primary reaction tubes, 4 were positive in the nested qPCR tested in 8 replicates (tubes 2, 4, 7 and 8; not shown) and were retested in TaqMan-based qPCR (e) and ddPCR (f). Shown are the results for positive control (pc, 10 copies/PCR reaction; 4 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), negative control (nc, untransduced hMSC; 8 replicates in TaqMan-based qPCR and 2 replicates in ddPCR), and tube 2 and 4 (16 replicates in TaqMan-based qPCR and ddPCR).

    Article Snippet: EV-transfer to recipient cells Recipient human BM-hMSC (2.5-5x104 ) unrelated to hMSC used for A .t .-transduction were seeded into T25-flasks (Greiner).

    Techniques: Fluorescence, Passaging, Incubation, Derivative Assay, SYBR Green Assay, Real-time Polymerase Chain Reaction, Positive Control, Polymerase Chain Reaction, Negative Control

    Alteration in protein levels and phosphorylation on short term AZD8055 treatment Cells at CPD 36 or CPD 73 were seeded into T25 flasks and grown in medium containing AZD8055 or DMSO for one week. After harvesting, cells were pelleted and lysed in RIPA with protease and phosphatase inhibitors, separated on SDS-PAGE then immunoblotted for the indicated proteins. ( A ) Components of the mTOR signaling pathway, ( B ) Cell cycle proteins. The panels on the right show longer exposure images of the same panels on the left, to highlight bands that are faint in the shorter exposure images.

    Journal: Aging (Albany NY)

    Article Title: Reversal of phenotypes of cellular senescence by pan-mTOR inhibition

    doi:

    Figure Lengend Snippet: Alteration in protein levels and phosphorylation on short term AZD8055 treatment Cells at CPD 36 or CPD 73 were seeded into T25 flasks and grown in medium containing AZD8055 or DMSO for one week. After harvesting, cells were pelleted and lysed in RIPA with protease and phosphatase inhibitors, separated on SDS-PAGE then immunoblotted for the indicated proteins. ( A ) Components of the mTOR signaling pathway, ( B ) Cell cycle proteins. The panels on the right show longer exposure images of the same panels on the left, to highlight bands that are faint in the shorter exposure images.

    Article Snippet: Cells were seeded at 2×105 per T25 flask (Greiner), or at 1×104 in 24 well plates (Greiner).

    Techniques: SDS Page

    Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Journal: PLoS ONE

    Article Title: Cell colony counter called CoCoNut

    doi: 10.1371/journal.pone.0205823

    Figure Lengend Snippet: Automate counting. The scoring is performed with 35 mm cell culture dishes for V79 (A) and HeLa (C) cells. T25 cell culture flasks are used in panels B and D for V79 and HeLa cells, respectively. Plotted automated counts are normalized to the manual scorings, namely the average of the pooled data of three scorers. Dotted lines are displayed only to guide the eye and do not imply any kind of prediction.

    Article Snippet: The count for viable cells was used for plaiting cells in either T25 flasks (25 cm2 cell culture area; Sarstedt, Skanderborg, Denmark) with ventilated caps containing 6 ml complete medium or in 35 mm cell culture dishes (Sigma-Aldrich) with 3 ml complete medium, respectively.

    Techniques: Cell Culture