Journal: Clinical and Vaccine Immunology : CVI
Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿
Figure Lengend Snippet: SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.
Article Snippet: ELISA was performed on serum samples from cattle to detect antibodies against four Treponema phagedenis -like spirochete isolates—HT201, YG3903R, HD26-43, and IZ6-2—isolated from cows with PDD raised in four different locations (three different islands) in Japan and two control type strains— T. phagedenis ATCC 27087 and T. denticola JCM 8225.
Techniques: SDS Page, Isolation