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    ATCC t phagedenis atcc 27087
    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. <t>phagedenis</t> ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;
    T Phagedenis Atcc 27087, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Journal: Journal of Clinical Microbiology

    Article Title: Genetic Heterogeneity among Strains of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis in Japan ▿

    doi: 10.1128/JCM.01574-08

    Figure Lengend Snippet: RAPD fingerprints of 26 PDD isolates and 2 type strains. Lanes and strains are as follows: 1, T. denticola JCM8225; 2, T. phagedenis ATCC 27087; 3, HT201; 4, YG3903R; 5, YG5618; 6, HG42; 7, IZ6-2; 8, IZ7-2; 9, CH6; 10, CH9; 11, HD21-66; 12, HD21R-R7;

    Article Snippet: T. phagedenis ATCC 27087 and all PDD isolates were catalase positive and oxidase negative.

    Techniques:

    Comparisons of ELISA mean ODs for five individual cow sera against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes isolated from cows with PDD.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: Comparisons of ELISA mean ODs for five individual cow sera against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes isolated from cows with PDD.

    Article Snippet: ELISA was performed on serum samples from cattle to detect antibodies against four Treponema phagedenis -like spirochete isolates—HT201, YG3903R, HD26-43, and IZ6-2—isolated from cows with PDD raised in four different locations (three different islands) in Japan and two control type strains— T. phagedenis ATCC 27087 and T. denticola JCM 8225.

    Techniques: Enzyme-linked Immunosorbent Assay, Isolation

    IgG antibody responses in sera of cows determined by ELISA, against T. phagedenis ATCC 27087 (a), T. phagedenis -like HT201 (b), T. phagedenis -like YG3903R (c), T. phagedenis -like HD26-43 (d), and T. phagedenis -like IZ6-2 (e). Group A, PDD-positive cows on PDD-positive farms ( n = 85); group B, PDD-negative cows on PDD-positive farms ( n = 33); group C, cows on PDD-free farms ( n = 33) for each ELISA. Each circle represents optical density at a wavelength of 492 nm. IgG levels against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes of groups A and B are significantly higher than that of group C, but there are no significant differences between groups A and B. S, significant ( P

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: IgG antibody responses in sera of cows determined by ELISA, against T. phagedenis ATCC 27087 (a), T. phagedenis -like HT201 (b), T. phagedenis -like YG3903R (c), T. phagedenis -like HD26-43 (d), and T. phagedenis -like IZ6-2 (e). Group A, PDD-positive cows on PDD-positive farms ( n = 85); group B, PDD-negative cows on PDD-positive farms ( n = 33); group C, cows on PDD-free farms ( n = 33) for each ELISA. Each circle represents optical density at a wavelength of 492 nm. IgG levels against T. phagedenis ATCC 27087 and four T. phagedenis -like spirochetes of groups A and B are significantly higher than that of group C, but there are no significant differences between groups A and B. S, significant ( P

    Article Snippet: ELISA was performed on serum samples from cattle to detect antibodies against four Treponema phagedenis -like spirochete isolates—HT201, YG3903R, HD26-43, and IZ6-2—isolated from cows with PDD raised in four different locations (three different islands) in Japan and two control type strains— T. phagedenis ATCC 27087 and T. denticola JCM 8225.

    Techniques: Enzyme-linked Immunosorbent Assay

    SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: SDS-PAGE profiles of whole-cell lysates of Treponema phagedenis -like spirochetes isolated from cattle with PDD. Lanes and isolates are as follow: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9; 10, HD21-66; 11, HD21-R7; 12, HD22-63; 13, HD22-R16; 14, HD26-43; 15, HD26-67; 16, HD26-R4; 17, HD27-4; 18, HD27-24; 19, HD27-R6.

    Article Snippet: ELISA was performed on serum samples from cattle to detect antibodies against four Treponema phagedenis -like spirochete isolates—HT201, YG3903R, HD26-43, and IZ6-2—isolated from cows with PDD raised in four different locations (three different islands) in Japan and two control type strains— T. phagedenis ATCC 27087 and T. denticola JCM 8225.

    Techniques: SDS Page, Isolation

    Silver-stained profiles of glycolipid of Treponema phagedenis -like organisms isolated from cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass marker shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: Silver-stained profiles of glycolipid of Treponema phagedenis -like organisms isolated from cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass marker shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Article Snippet: ELISA was performed on serum samples from cattle to detect antibodies against four Treponema phagedenis -like spirochete isolates—HT201, YG3903R, HD26-43, and IZ6-2—isolated from cows with PDD raised in four different locations (three different islands) in Japan and two control type strains— T. phagedenis ATCC 27087 and T. denticola JCM 8225.

    Techniques: Staining, Isolation, Marker

    Western blot reaction profiles of IgG antibody in sera from two individuals, cow YG3903 (A) and cow IZ6 (B), harboring PDD lesions, against glycolipid of Treponema phagedenis -like organisms isolated from different cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Analysis of the IgG Immune Response to Treponema phagedenis-Like Spirochetes in Individual Dairy Cattle with Papillomatous Digital Dermatitis ▿

    doi: 10.1128/CVI.00464-09

    Figure Lengend Snippet: Western blot reaction profiles of IgG antibody in sera from two individuals, cow YG3903 (A) and cow IZ6 (B), harboring PDD lesions, against glycolipid of Treponema phagedenis -like organisms isolated from different cattle with PDD. Lanes and isolates are as follows: M, prestained molecular mass markers shown in kilodaltons; 1, T. phagedenis type strain ATCC 27087; 2, HT201; 3, YG3903R; 4, YG5618; 5, HG42; 6, IZ6-2; 7, IZ7-2; 8, CH6; 9, CH9.

    Article Snippet: ELISA was performed on serum samples from cattle to detect antibodies against four Treponema phagedenis -like spirochete isolates—HT201, YG3903R, HD26-43, and IZ6-2—isolated from cows with PDD raised in four different locations (three different islands) in Japan and two control type strains— T. phagedenis ATCC 27087 and T. denticola JCM 8225.

    Techniques: Western Blot, Isolation

    Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Journal: Infection and Immunity

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    doi: 10.1128/IAI.00019-07

    Figure Lengend Snippet: Antibody response and cross-reactivity. Antibody response and cross-reactivity of antibodies produced were measured by ELISA. Bars indicate the organism against which the serum run on the plate was raised, as follows: 1A, clear open bar; 3A, small gray dots; 4A, clear bar with horizontal stripes; 5B, black filled bar; T. denticola , cross-hatched bar; T. phagedenis , clear bar with left diagonal stripes. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. The label inside the graph in the top corner indicates the organism coating the ELISA plate. Top left, T. denticola (T dent). Top right, T. phagedenis (T phag). Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Bottom left, PDD isolate 4A. Bottom right, PDD isolate 5B. There were 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Article Snippet: T. phagedenis (ATCC 27087) was grown in PY medium with cocarboxylase and serum (ATCC medium 1828).

    Techniques: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Journal: Infection and Immunity

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    doi: 10.1128/IAI.00019-07

    Figure Lengend Snippet: Time course of lesion development. Mice received treponemes subcutaneously at inoculums of 10 9 (closed square, solid line), 10 10 (open square, dashed line), 10 11 (closed circle, solid line), and 10 11 formalin-treated (open circle, dashed line) spi. Lesion development was monitored for 34 days. T. denticola (A) was used as a pathogenic control. T. phagedenis (B) served as a nonpathogenic control. PDD isolates 1A (C), 3A (D), 4A (E), and 5B (F) differed in peak lesion size, days to peak lesion size, and lesion duration. A mixed inoculum (H) composed of all of the PDD isolates together (1A, 3A, 4A, and 5B at 1:1:1:1) induced lesions smaller than those induced by the individual isolates. In all of the groups, formalin killing (f-k) of the spirochetes severely hampered lesion development. There were 6 to 10 mice per group.

    Article Snippet: T. phagedenis (ATCC 27087) was grown in PY medium with cocarboxylase and serum (ATCC medium 1828).

    Techniques: Mouse Assay

    Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Journal: Infection and Immunity

    Article Title: Lesion Formation and Antibody Response Induced by Papillomatous Digital Dermatitis-Associated Spirochetes in a Murine Abscess Model ▿

    doi: 10.1128/IAI.00019-07

    Figure Lengend Snippet: Antibody subclass production. The relative amounts of IgG1 (gray right diagonal lined bar), IgG2a (gray hatched bar), IgG2b (clear right diagonal lined bar), and IgG3 (clear open bar) produced in response to each isolate were determined by ELISA. Each value on the x axis is the number of spirochetes in the inoculum for each serum. FK (f-k) spirochetes were inoculated at 10 11 spi. Upper left, T. denticola . Upper right, T. phagedenis . Middle left, PDD isolate 1A. Middle right, PDD isolate 3A. Lower left, PDD isolate 4A. Lower right, PDD isolate 5B. N.D. = not done. Data are the mean (± SEM) of 6 to 10 mice per group. Differences in the y -axis scale do not indicate differences in the antibody titers induced by the different spirochetes. See Materials and Methods.

    Article Snippet: T. phagedenis (ATCC 27087) was grown in PY medium with cocarboxylase and serum (ATCC medium 1828).

    Techniques: Produced, Enzyme-linked Immunosorbent Assay, Mouse Assay