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Image Search Results
Journal: Frontiers in immunology
Article Title: Automated Clinical Grade Expansion of Regulatory T Cells in a Fully Closed System.
doi: 10.3389/fimmu.2019.00038
Figure Lengend Snippet: FIGURE 1 | Quantification of CD4+CD25highCD127lowFOXP3+ Treg and other lymphocyte subsets before and after CD8−CD25high enrichment. (A) Phenotyping of initial cell source (leukapheresis), CD8+ depleted intermediate product and final CD25high Treg enriched fraction by flow cytometry. Shown are representative flow cytometry plots for one of the donors. (B) Relative percentage of Treg and contaminating CD4+CD25−/low (CD4 resting), effector memory CD4+CD25+CD127+CD45RO+ (CD4 Teff), CD8 cytotoxic T cells, B cells, NK cells and monocytes. Shown are individual and mean percentages (solid line) of CD45+ leukocytes of each subset for n = 8 Treg isolations. Error bars = SD.
Article Snippet: After depletion of CD8+ cells, the intermediate product was enriched for the
Techniques: Cytometry