Journal: eLife

Article Title: Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord

doi: 10.7554/elife.83108

Figure Lengend Snippet: Figure 4. Serial block-face scanning electron microscopy (SBF-SEM) analyses with COX4-dAPEX2 and SYP-HRP labeling reveal structures and recurrent connections of cerebrospinal fluid-contacting neurons (CSF-cNs). (A, B) Representative light microscopic images of 3, 3'-diaminobenzidine (DAB)- stained spinal cord sections of Pkd2l1-Cre mice with injection of adeno-associated virus (AAV)-Syn-DIO-COX4-dAPEX2 (A) or AAV-Syn-DIO-SYP-HRP (B). (C) A low-magnification electron microscopic image of the cervical cord around the central canal (Cc) in Pkd2l1-Cre mice injected with AAV-Syn-DIO-

Article Snippet: AAV plasmids were generated as follows: for pAAV- Syn- mCherry, mCherry of pmCherry- C1 plasmid (Clontech) was subcloned into pAAV- hSyn- EGFP (Addgene, #50465) in a substitution of EGFP; for pAAV- Syn- DIO- COX4- dAPEX2 and pAAV- Syn- DIO- SYP- HRP, COX4- dAPEX2 and SYP- HRP derived from pAAV- EF1a- COX4- dAPEX2 (Addgene, #117176) and pAAV- EF1a- SYP- HRP (Addgene, #117185) were subcloned into pAAV- Syn- DIO- MCS that had multicloning sites (MCS) between the DIO site of pAAV- Syn- DIO- mCherry (Addgene, #50459) in a substitution of mCherry; and for pAAV- Syn- DIOChR2- mCherry, ChR2(H134R)- mCherry derived from pAAV- CAG- ChR2(H134R)- mCherry (Addgene, #100054) was subcloned into pAAV- Syn- DIO- MCS plasmid. pAAV- hSyn- EGFP (Addgene, #50465), pAAV- CAG- EGFP (pENN.AAV.CB7.CI.eGFP.WPRE.rBG; Penn vector core, #AV- 1- PV1963), and pAAVSyn- DIO- mCherry (Addgene, #50459) were further used for AAV production.

Techniques: Blocking Assay, Electron Microscopy, Labeling, Staining, Injection, Virus

Journal: eFood

Article Title: Docosahexaenoic Acid and Nervonic Acid Synergically Enhance Cognitive Memory in Normal Mice via Brain Fatty Acids Remodeling

doi: 10.1002/efd2.70059

Figure Lengend Snippet: FIGURE 3 | DHA and NA enhanced c‐Fos expression in the hippocampus of normal mice. (A) Representative c‐Fos immunopanned photos in the hippocampus. (B) The number of c‐Fos+ neurons in the hippocampal DG area. (C) The expression of PSD95 in the hippocampus. (D) The expression of synaptophysin (SYP) in the hippocampus. *p < 0.05, **p < 0.01 vs control group. One‐way ANOVA post‐hoc Dunnett's test (mean ± SEM, n = 4).

Article Snippet: Immunostaining was performed using monoclonal antibodies against c‐Fos (1:1000; Cell Signaling Technology, cat#2250S) and synaptophysin (SYP, 1:400; Santa Cruz, sc‐17750), polyclonal antibody against PSD95 (1:200; Invitrogen, cat#51‐6900), followed by incubation at 4°C for 24 h. Goat anti‐ mouse (Alexa Fluor 594, 1:500, ab150116) and goat anti‐rabbit (Alexa Fluor 488, 1:500, ab150077) IgG were used as secondary antibodies and incubated in the dark at room temperature for 2 h. Fluorescent images were captured using an ECHO Revlove fluorescence microscope (ECHO, San Diego, California, USA) and quantitatively analyzed using ImageJ software (NIH, Bethesda, Maryland, USA).

Techniques: Expressing, Control

Journal: Communications Biology

Article Title: Integrated multi-omics reveal important roles of gut contents in intestinal ischemia–reperfusion induced injuries in rats

doi: 10.1038/s42003-022-03887-8

Figure Lengend Snippet: a Principal component analysis (PCA) score plot derived from metabolites abundance. b The volcano plots displayed the statistical significance ( P value) versus the magnitude of change (Log2 fold change). Features with Wilcoxon test p value <0.05 and absolute Log2 fold change (Log2FC) >1 were deemed to be significantly different. c Shown here are gut metabolites that were significantly altered during IIR and were not returned to normal levels at IIR6h, i.e., significantly different between IIR6h compared to Sham. Between-group comparisons were performed using the Wilcox test, n = 10 biologically independent animals in Sham group and n = 15 in IIR0h group, n = 10 in IIR6h group; * p < 0.05; ** p < 0.01; *** p < 0.001; NS not significant. d Immunohistochemical level of several molecular markers for the intestinal neuroendocrine system significantly changed during IIR: nitric oxide synthase (iNOS), C-Kit, synaptophysin (SYP), protein gene product 9.5 (PGP9.5), and substance P (SP). Between-group comparisons were performed using the Wilcoxon test, n = 3 biologically independent animals; * p < 0.05; ** p < 0.01; NS not significant. The box re p resents the median, 25th, and 75th percentiles and the error bars indicate the 5th and 95th percentiles.

Article Snippet: The paraffin sections of the intestinal were immunostained with antibodies nitric oxide synthase Rabbit pAb (# A0312), C-Kit (#A0357), synaptophysin (#A6344), protein gene product 9.5 (#A19101), substance P (#A13550) (ABclonal Biotech Co., Ltd, Wuhan, China), which were commonly used molecular markers for neuroendocrine system and enteric nervous system – .

Techniques: Derivative Assay, Immunohistochemical staining

Journal: Oncology Letters

Article Title: Synchronous liver metastasis at initial diagnosis of adrenal pheochromocytoma by CT: A case report

doi: 10.3892/ol.2024.14462

Figure Lengend Snippet: Postoperative pathological analysis. (A) HE staining results of adrenal mass. (B) HE staining results of liver mass. (C-E) Immunohistochemistry of liver mass: (C) Chromogranin A (+) and synaptophysin (+); (D) α-fetoprotein (−); and (E) cytokeratin (−) (magnification, ×400). Blue staining indicates negative expression, whereas brown staining indicates positive expression.

Article Snippet: The antibodies against chromogranin A (cat. no. #ZM-0076; dilution 1:200), synaptophysin (cat. no. #ZM-0246; dilution 1:200), CK7 (cat. no. #ZA-0573; dilution 1:200), CK20 (cat. no. #ZA-0574; dilution 1:200), α-fetoprotein (cat. no. #ZM-0009; dilution 1:200), and α-inhibin (cat. no. #ZM-0460; dilution 1:200) were from Origene Technologies, Inc.

Techniques: Staining, Immunohistochemistry, Expressing