Journal: Neural Regeneration Research

Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

doi: 10.4103/NRR.NRR-D-24-00628

Figure Lengend Snippet: NRMS upregulates transcriptional levels of genes related to synaptic plasticity in damaged region of SCI rats. (A) The functional classification of different genes in the SCI + NRMS and SCI + SS groups by GO enrichment analysis. (B) The functional classification of different genes between the SCI + NRMS and SCI + SS groups by KEGG database. (C–F) The relative levels of Syn2 , Syp , GAP43 , and MAP2 mRNA normalized by the Sham group. The bold lines, upper boundaries, and lower boundaries represent the medians, 75 th percentiles, and 25 th percentiles, respectively. Whiskers extend 1.5 times interquartile range ( n = 4 per group). * P < 0.05, vs . Sham group; # P < 0.05, vs . SCI + SS group. GAP43: Growth-associated protein 43; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; MAP2: microtubule-associated protein 2; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation; Syn2: synapsin II; Syp: synaptophysin.

Article Snippet: Moreover, the major functions of Synapsin II are focused on the regulation of Ca 2+ -dependent plasticity and neuronal outgrowth in glutamatergic neurons (Matos et al., 2019).

Techniques: Functional Assay

Journal: Neural Regeneration Research

Article Title: Nerve root magnetic stimulation regulates the synaptic plasticity of injured spinal cord by ascending sensory pathway

doi: 10.4103/NRR.NRR-D-24-00628

Figure Lengend Snippet: NRMS alters expression of proteins associated with synaptic plasticity in the injured spinal cord of rats. (A) Representative western blots show the expression levels of PSD95, GAP43, Synapsin I and Synapsin II collected from the damaged area of spinal cord on day 22 after SCI. (B–E) The semi-quantified results of PSD95, GAP43, Synapsin I, and Synapsin II protein levels. All experiments were repeated three times. Data are expressed as mean ± SD ( n = 5 per group). ** P < 0.01, *** P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, vs . SCI + SS group. GAP43: Growth-associated protein 43; PSD95: postsynaptic density protein-95; NRMS: nerve root magnetic stimulation; SCI: spinal cord injury; SS: sham stimulation.

Article Snippet: Moreover, the major functions of Synapsin II are focused on the regulation of Ca 2+ -dependent plasticity and neuronal outgrowth in glutamatergic neurons (Matos et al., 2019).

Techniques: Expressing, Western Blot

Journal:

Article Title: Nerve Agent Exposure Elicits Site-Specific Changes in Protein Phosphorylation in Mouse Brain

doi: 10.1016/j.brainres.2010.04.034

Figure Lengend Snippet: Effect of DFP on a panel of neuronal phosphorylation sites comprising the CNSProfile technology platform as measured in striatum and hippocampus of male FVB mice.

Article Snippet: Immunoblotting was carried out using phosphorylation state-specific antibodies (and respective phosphorylation state insensitive antibodies for measuring the total protein levels) raised against pT34, pT75, pS102 or pS137 of DARPP-32 and pS94 of spinophilin (ITI); pS133 of CREB (Upstate Biotechnology, Charlottesville, VA); pT183 of ERK1/2 (Promega, Madison, WI); pS897 of the NMDA receptor NR1 subunit (Upstate); pS831 or pS845 of the AMPA receptor GluR1 subunit (Upstate); S9 of the GSK3β kinase (BD Biosciences); S473 or T308 of the protein kinase Akt (BD Biosciences); S549 or S603 of the synaptic vesicle protein, synapsin I/II (Dr. Angus Nairn, RU and BD Biosciences); or pS40 of tyrosine hydroxylase (Chemicon, Temecula, CA).

Techniques:

Journal:

Article Title: Nerve Agent Exposure Elicits Site-Specific Changes in Protein Phosphorylation in Mouse Brain

doi: 10.1016/j.brainres.2010.04.034

Figure Lengend Snippet: Sites of phosphorylation monitored in brain after DFP treatment using CNSProfile.

Article Snippet: Immunoblotting was carried out using phosphorylation state-specific antibodies (and respective phosphorylation state insensitive antibodies for measuring the total protein levels) raised against pT34, pT75, pS102 or pS137 of DARPP-32 and pS94 of spinophilin (ITI); pS133 of CREB (Upstate Biotechnology, Charlottesville, VA); pT183 of ERK1/2 (Promega, Madison, WI); pS897 of the NMDA receptor NR1 subunit (Upstate); pS831 or pS845 of the AMPA receptor GluR1 subunit (Upstate); S9 of the GSK3β kinase (BD Biosciences); S473 or T308 of the protein kinase Akt (BD Biosciences); S549 or S603 of the synaptic vesicle protein, synapsin I/II (Dr. Angus Nairn, RU and BD Biosciences); or pS40 of tyrosine hydroxylase (Chemicon, Temecula, CA).

Techniques: Activity Assay